Day 3 :
- Track 1: Advanced trends for the future of herbal drugs and its products
Track 5: Toxicology Studies of Plant Products
Track 6: Ethnopharmacology
Track 7,8: Phytochemistry and Phytoconstituents, Industrial Pharmacognosy
Location: Hyderabad International Convention Centre, India
Session Introduction
Gideon O Alade
Niger Delta University, Nigeria
Title: Antifertility activity of Bambusa vulgaris aqueous leaf extracts in male wistar rats
Time : 11:05-11:25
Biography:
Will be uploaded soon.
Abstract:
Globally, there is a need for family planning as a result of increasing human population growth rate with its undesirable impact on the environment, economic growth and poverty in several parts of the world most especially in the underdeveloped countries. Ninety percent of the world’s contraceptive users are women, probably due to the slow progress in development and limited possibilities of male contraceptives. Several medicinal plants have been implicated as antifertility in males, but only a few have been investigated. Bambusa vulgaris L. (Poaeceae), commonly known as “Bamboo” is found in Tropical and Sub-tropical areas It is commonly referred to as ‘Oparun’, ‘Iko’, and ‘Atosin’ among the Yoruba, Bini and Igbo tribes of Nigeria respectively. Ethnobotanical study of antifertility medicinal plants used by the people of Kathijavadi village in India revealed that the extract of B. vulgaris leaves is taken orally to reduce sperm count. Therefore, the study seeks to provide scientific justification to the acclaimed sperm reducing potentials of B. vulgaris leaf in male wistar rats. Male wistar rats were administered with 250 (n=12) and 500 mg/kg (n=24) of B. vulgaris leaf ethanol extract for 14 and 28 days, distilled water acting as negative control and 6 rats each were sacrificed at the end of 14 and 28 days separately. They were evaluated for sperm concentration, motility, testosterone (T), leutinizing (LH) and follicle stimulating hormones (FSH), histology of the testes was also carried out. The remaining of the rats administered with 500 mg/kg for 28 days were kept and allowed free access to feed and water while extract administration was withdrawn. Six rats were sacrificed weekly and sperm concentration and motility were evaluated for probable reversal of activity. There was 42 and 31 % reduction in sperm count at 14 and 28 days respectively in rats administered with 250 mg/kg while at 500 mg/kg dose, the percentage reduction in sperm count was 60 % and there was almost a complete reversal of activity 14 days after cessation of treatment. The result justified the ethnobotanical claim of the use of B. vulgaris leaf in reducing sperm count.
Prasad Thakurdesai
Indus Biotech Private Limited, India
Title: Therapeutic efficacy of trigonelline-based standardized extract of fenugreek seeds on Levodopa induced dyskinesia in 6-OHDA lesioned rat model of Parkinson’s disease
Time : 11:25-11:45
Biography:
Prasad Thakurdesai is GM- Scientific affairs at Indus Biotech Private Limited, Pune, India since 2008. He completed his PhD in 2001 from Nagpur University, Nagpur, India. He has experience of 17 years in academic institutions and 9 years in pharmaceutical industries. He has guided 2 PhD and more than 30 M.Pharm. research students. He has published more than 77 articles in International and National Peer reviewed Journals, presented 49 papers in international conferences. His research interests include Pharmacology, Toxicology, Nutraceuticals and health related Quality of life (HRQoL).
Abstract:
The objective of the present work is to explore the therapeutic efficacy and possible mechanism of trigonelline-based standardized extract of fenugreek seeds (IBHB) on L-dopa induced dyskinesia (LID) in 6-hydroxydopamine (6-OHDA) lesioned rat model of Parkinson’s disease (PD). The motor symptoms of PD and LID were induced in rats with single unilateral intracerebroventricular injections of 6-OHDA in the striatum area of brain (PD) on day-0 and daily treatment with L-DOPA + Benserazide from Day-29 to Day-56 (LID) respectively. Rats were then randomly divided into groups and administrated with subacute treatment either with vehicle, amantadine (40 mg/kg, i.p.) or IBHB (15, 30 or 60 mg/kg, oral) for next 45 days and then sacrificed for collection of brain striatal samples for ex vivo experiments. Subacute treatment of IBHB showed dose-dependent reduction in behavioral parameter scores (abnormal involuntary movements (AIMS), grid test, catalepsy bar test, Forelimb adjusting steps) as compared to vehicle treated LID group. During ex vivo experiments, IBHB showed restoration of upregulated neurotransmission (serotonin and dopamine turnover and 3-O-Methyldopa levels), immunoreactivity (FosB and 5HT2c), and mRNA expressions (c-FOS, CO-1, Homer-1, Parkin, Pdyn, Penk and PINK1) and downregulated mitochondrial respiratory chain complexes activity and mRNA expression of JunD in striatum as compared to vehicle treated LID control rats. In conclusion, subacute treatment of IBHB showed promising therapeutic anti-dyskinetic efficacy in preclinical model of LID in rats and supports the utility for the improved treatment of LID and PD.
Swaroopa Rani N Gupta
Brijlal Biyani Science College, India
Title: Polarographic study of meta-hydroxy acetanilide and its determination
Time : 11:45-12:05
Biography:
Swaroopa Rani N Gupta has done PhD in Chemistry from Nagpur University, Maharashtra, India in 1993. She is an Associate Professor in the Department of Chemistry, Brijlal Biyani Science College, Amravati, Maharashtra, India. She has published more than 15 papers in reputed international journals. She has presented papers at international conferences at India, Singapore, London, Dubai, Hong Kong, Mauritius, Tashkent and has been serving as Technical Committee Member of international conferences at Singapore, UK, Dubai, Hong Kong, Mauritius, Korea, Turkey, New Zealand, etc.
Abstract:
Present paper deals with polarographic study of effect of maxima suppressors and supporting electrolytes on anodic waves of meta-hydroxy acetanilide and polarographic determination of meta-hydroxy acetanilide under optimum concentration of maxima suppressors and supporting electrolytes. The polarographic method has been developed to study qualitatively the effect of maxima suppressor (fuchsin) and supporting electrolyte (nitric acid) on oxidation wave of meta-hydroxy acetanilide. Polarograms of system were recorded from 500 to 1300 by using Rotating Platinum Micro Electrode as anode and Saturated Calomel Electrode as cathode on DC Recording Polarograph using Omniscribe recorder. It shows a similar behavior to that observed for paracetamol. It produces anodic wave at rotating platinum electrode. The oxidation yields the 3-N-acetylaminosemiquinone and represents a reversible reaction. Polarographically a value of 900 mV is found for decomposition potential of meta-hydroxy acetanilide. Wave analysis point to one electron-step for each wave. Waves of meta-hydroxy acetanilide are only proportional to concentration at low concentrations.
B Janarthanam
Poonga Biotech Research Centre, India
Title: Studies on phytochemical analysis, proximate analysis and antioxidant activity of Cucumis melo seed extract
Time : 12:05-12:25
Biography:
B Janarthanam has completed his PhD degree in the subject of Botany from University of Madras. He has also worked as Chief Scientist & Project Principal Investigator in OMNI GREEN Organic Bio Park Private Limited, as Project Co-PI & Research Associate in PG & Research Department of Botany, Pachaiyappa’s College, Chennai and as Project Co-PI & Junior Research Fellow in Shri A M M Murugappa Chettiar Research Centre, Tharamani, Chennai. He has more 10 years of research experience in various fields of Secondary Metabolite production, Enzyme assays, Antioxidant activity and plant tissue culture techniques. He has served as Project Principal Investigator & Co-Principal Investigator in projects sponsored by Department of Science and Technology, New Delhi, National Medicinal Plant Board, New Delhi and M/s Shrivathsa Herbs & Species Pvt. Ltd. He has guided more than 40 project students from various colleges and Universities for their BTech/MSc/MTech dissertation work. He has 18 international scientific publications in reputed journals and has participated and presented his research findings in more than 25 international and national conferences. He has also given oral presentations as Invited Speaker in the “Asia Pacific Conference of Plant Tissue Culture and Agribiotechnology” (APaCPA 2007) held in Malaysia and in the “International conference on Natural Products and Biomedical Technology”, ICNPBT 2011, at Annamalai University.
Abstract:
The present study was performed to investigate the phytochemical screening, proximate composition, total phenol, total flavonoid content and DPPH assay of seed extracts of Cucumis melo. Phytochemical screening of various extracts such as aqueous, ethanol, chloroform, acetone and petroleum ether of seed extracts, revealed the presence of tannins, saponins, phenols, flavonoids, cardiac glycosides, terpenoids, alkaloids and steroids. Butylated Hydroxy Toluene (BHT), Gallic acid (GA) and Quercetin (Q) were taken as standard in case of antioxidant activity, phenol and flavonoid content respectively. Total phenol and flavonoid contents were quantitatively estimated. Total phenolic content was estimated by Folin–Ciocalteau method and flavonoid content measured by Aluminum chloride method. Proximate analysis such as total protein, carbohydrate, ash, crude fibre and moisture contents were carried out following standard methods. The seed extracts were evaluated for antioxidant activities by DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity. Among five different solvents used, maximum antioxidant activity was found in aqueous extract (73.2%) followed by others. The total phenol and flavonoid content in seed extract were found to be 23.32 mg gallic acid equivalents (GAE)/g and 63.1 mg Quercetin Equivalent (QE)/g, respectively. The aqueous extracts from dry powdered seed of Cucumis melo had superior level of antioxidant activity. The powerful antioxidant effect is attributed to the greater amount of phenols and flavonoids compound in the ethanolic peel extracts of Cucumis melo.
Juliao A Monjane
Eduardo Mondlane University, Mozambique
Title: Phytochemical investigation of Cadaba natalensis roots
Time : 12:25-12:45
Biography:
Juliao A Monjane is a PhD candidate at Lund University in Sweden. He is currently in the 3rd year of PhD studies. His research project is related with isolation and characterization of compounds with interesting biological activities derived from selected Mozambican medicinal plants used locally in antibacterial therapy. He teaches courses for Bachelor’s students at Eduardo Mondlane University in Mozambique. His research interests are focused on areas related with Natural Products, Organic Synthesis, and Nuclear Magnetic Resonance Spectroscopy.
Abstract:
The roots of Cadaba natalensis (Capparaceae family), known as Tssatssassana or Ndoda Ya Munhama in Southern Mozambique have been used to treat tuberculosis. Several species of the genus Cadaba have been studied phytochemically, although no previous studies have been carried out on C. natalensis. The aim of this work was to isolate and characterize some phytochemical constituents present in the roots of this plant. The isolation of the compounds was done by chromatographic methods and two novel compounds together with five known compounds were isolated. The novel compounds were by spectroscopic techniques characterized as dimeric a tetrahydroisoquinoline similar to thalifoline and 4-oxazolidine derivatives, and a 2-oxazolidine derivative that for the first time was obtained from a natural source. This is the first study in which the rare five membered ring structures (2 and 4-oxazolidine derivatives) are isolated from a plant, although similar compounds have previously been isolated from marine sources. Beside these three compounds, also thalifoline, N-methylcorydaldine, β-sitosterol, and nepetin were obtained. The 2 and 4-oxazolidine derivatives have been reported to possess antimicrobial, antibacterial, and anticancer activities, while tetrahydroisoquinoline derivatives can have vasorelaxant activity. The structures of isolated compounds were characterized by 1D and 2D NMR spectroscopy, HRESI-MS, and by the comparison with the reported spectroscopic data for the known ones.
Dharmesh R Chejara
University of the Witwatersrand, South Africa
Title: Natural polymer based novel micro porous soft hydrogel system: A potential viscosupplimentation for biomedical applications
Time : 12:45-13:05
Biography:
Dharmesh R Chejara has completed his PhD at the age of 29 years from Academy of Scientific and Innovative Research (AcSIR), New Delhi and PhD research work was carried out at CSIR-CSMCRI, Bhavnagar, Gujarat, India. He is doing his postdoctoral research since July 2014 from University of Witwatersrand, Johannesburg South Africa. He has published more than 10 papers in reputed journals
Abstract:
We report herein, synthesis of natural polymer sodium alginate (Na-Alg) based novel soft hydrogel system where aminocaproic acid (ACA) was attached on to the polymer using carboddimide coupling chemistry. The designed soft hydrogel was characterized using various analytical techniques such as Fourier transform infrared spectroscopy (FT-IR), Nuclear magnetic resonance spectroscopy (NMR), Scanning electron microscopy (SEM) and Differential scanning calorimetry (DSC). This soft hydrogel was further investigated for stress responsiveness using different rheological studies which confirmed the thixotropic nature of the gel, which is rendered flowable (fluid) on applying physical stress and recovers its ”rigid” gel structure upon removal of these applied stimuli. In addition the hydrogel was found to be nontoxic in nature. This approach of synthesizing a natural polmer-based materials could be applicable to a broad variety of other natural polymers and may have a potential utility as a viscosupplimentation for orthopedic implants in the field of biomedical applications.
Ciddi Veeresham
Kakatiya Univeristy, India
Title: Development and Evaluation of selected Indian medicinal plant based poly herbal Anti-Aging cream
Biography:
Will be uploaded soon.
Abstract:
Practice of using herbal remedies as a strategy against aging skin has been evolved as an imperative step to accomplish comprehensive skin rejuvenation. Present study is aimed to prepare and evaluate a poly herbal cream containing standardized extracts of Aloe vera (A), Ocimum sanctum (O) and Punica granatum peel (P) at different ratios. Santalum album, Citrus aurantium, Olea europa, Prunus amygdalus oils, Cetyl alcohol and Stearic acid were used in a ratio of 0.1:0.1:1.0:0.5:0.5 and 3.0 respectively, along with preservatives to formulate a cream base for formulation water in oil (W/O) creams i.e. F1 to F6. Prepared formulations were evaluated for formulation parameters like pH, viscosity, spreadabilty, extrudability and stability. Further, formulations were also evaluated for anti-oxidant, anti-microbial and advanced glycation end product inhibition (AGE) properties. Formulations F3-F6 have shown no phase separation upon storage. Formualtions F3 and F6 have featured lower spreadability and extrudabiltiy. The order of anti-oxidant ability of the all formulations found as F6>F5>F4>F3>F2>F1. Formulations F4 and F5 shown comparable AGE inhibitory and anti-microbial effects to standards Aminoguanidine and Neomycin sulphate cream, respectively, however F4 shown higher AGE inhibitory effect vis-a-vis with F5. In human volunteer’s study, the formulations F4 and F5 shows no redness, edema, inflammation and irritation during irritancy studies. The synergistic effects of formulation F4 containing A: O: P (each 1.6% w/w) has addressed hyperpigmentation, erythema, wrinkles and firmness of skin more significantly than F5. These finding indicate the significant anti-aging potential of examined poly herbal anti aging cream.
P Subramanian
Drug Standardisation Unit, India
Title: Pharmacognosy & physico – chemical evaluation of homoeopathic drug erigeron canadensis linn
Time : 13:35-13:55
Biography:
Will be uploaded soon
Abstract:
Herbal products are composed of many diverse chemical constituents of immense therapeutic value and capable of variation. Standardisation is therefore extremely essential and mandatory for global acceptance. Erigeron canadensis Linn., is an erect annual herb belonging to the family Asteraceae. It is also known in English as ‘Canada Fleabane’. Aerial parts are used in homoeopathy for bruises, cough, dysuria, gonorrhoea, haemorrhages, haemorrhoids, spermatorrhoea and wounds. The leaves are 2.5 – 4 cm long, sessile, linear obovate with rough surface. Stems are 2 to 5 mm thick, ridged with coarse surface and densely hairy. Epidermal cells in surface possess wavy to sinuate sides; stomata are anomocytic, anisocytic and tetracytic; trichomes are uniseriate conical in structure. The midvein in transection is flat on adaxial and ribbed on abaxial. Palisade is 2- layered. A secretory cavity is present beneath the central vascular bundle. Stem in transection is rounded and undulated, covered by uniseriate conical hair. The vascular tissue is made of several V. bundles in a ring capped by sclerenchyma sheath. Centrally pith is present with a disintegrated lumen. Mature stem shows outermost epidermis replaced by cork. Secondary xylem is well developed and phloem is reduced. A vascular cambium is present. Pollen grain is a monad, prolate spheroided or sub prolate with spinescent surface. The powder microscopic and organoleptic characters are presented. Physico – chemical parameters of raw drugs viz., extractive values, ash values, formulation besides wt.per ml., total solids, alcohol content along with HPTLC fingerprinting and UV studies were undertaken of the mother tincture for the first time.
P Padma Rao
Drug Standardisation Unit, India
Title: Pharmacognostic standardisation of rumex crispus linn. in homoeopathic
Time : 13:55-14:15
Biography:
Will be uploaded soon
Abstract:
Plant kingdom represents an extraordinary reservoir of molecules, that can be beneficial to mankind. Rumex crispus L., is a perennial shrub belonging to the family Polygonaceae. It is also known in English as ‘Curled dock’ or ‘yellow dock’. It is a native of Europe and also occurs worldwide. The roots are useful in various therapeutic conditions viz., abortion, asthma, bronchitis, catarrh, corns, coryza, cough, and diarrhoea. The roots are 0.5 to 1.3 cm thick, blackish, brown, tortuous with irregular striations and with long, wiry rootlets. The outermost phellem is 8-10 layered, shrivelled and discontinuous. Phelloderm occurs in two zones with outer possessing elongated cells in undulated layers containing dense resinous matter and sphaeraphides. Secondary phloem is upto 25 celled and interrupted by 1-3 seriate medullary rays. The central cortex is extensive and traversed with vascular strands. Pith is present at the centre. The powder microscopic and organoleptic characters are presented. Physico – chemical parameters of raw drugs viz., extractive values, ash values, formulation besides wt.per ml., total solids, alcohol content along with HPTLC fingerprinting and UV studies were undertaken of the mother tincture for the first time.
Chittipong Tipbunjong
Mahidol University, Thailand
Title: Enhancement of C2C12 myoblast proliferation and differentiation by diarylheptanoids form Curcuma comosa Roxb
Time : 14:15-14:35
Biography:
Chittipong Tipbunjong is a Ph.D. candidate at the Department of Anatomy, Faculty of Science, Mahidol University. He receives a scholarship from the Office of the Higher Education Commission. At 2013-2014, he was a visiting scholar student at the Department of Physiology, College of Medicine, University of Kentucky.
Abstract:
Enhancement of C2C12 myoblast proliferation and differentiation by diarylheptanoids form Curcuma comosa Roxb.: Diarylheptanoids is a phytoestrogen that exhibits numerous biological activities. However, its effect on myogenesis is not known. Previously, it has been reported that estrogen improved muscle regeneration after injury and that mouse myoblasts, C2C12, expressed estrogen receptors. This study, therefore, aims to determine the effect of 1-(4-hydroxyphenyl)-7-phenyl-(6E)-6-hepten-3-one, which is extracted from Curcuma comosa Roxb. and has an estrogenic activity comparable to that of genistein, on myoblast differentiation. Treatment with this compound increased the expressions of myogenin and myosin heavy chain (MHC) proteins determined by western blotting and immunofluorescence staining. These effects were blocked by ICI 182,780 and ER-ï¡ siRNA suggesting that ER-ï¡ is involved in the enhancement of myoblast differentiation. At the early stage of differentiation, phosphorylated ERK, Akt and p38MAPK were increased. However, blockades with their specific inhibitors showed that only phospho-Akt and phospho-p38MAPK, but not phospho-ERK, were associated with myoblast differentiation. The compound also increased phosphorylation of P70S6Kinase, GSK3-β, β-catenin, and NF-ĸB. Interestingly, blockades with Akt inhibitor, Rapamycin, and SB203580 revealed mTOR/P70S6Kinase and GSK3-β/β-catenin are the downstream of Akt, but NF-ĸB is under the p38 MAPK and no crosstalk between Akt and p38 MAPK during differentiation. It is concluded that the diarylheptanoid enhances C2C12 myoblast differentiation by increasing MHC and myogenin levels through ER-α mediated Akt/mTOR/P70S6Kinase, Akt/GSK3-β/β-catenin, and p38 MAPK/NF-ĸB signaling.
Ashok Surana
Andhra University, India
Title: Leaders in holistic healing and body therapeutics
Time : 14:35-14:55
Biography:
Ashok has completed Bachelorette of Engineering from JNTU, Andhra University and is a alternative medicine enthusiast. He has been researching the healing properties of spices and herbs for more than fifteen years now. The dominant idealogy stemmed from the famous adage – live and let live. If we dont overwhelm our bodies with chemicals and let let it heal itself, it has the intelligence to keep the harmful bacteria at bay.
Abstract:
I have studied the medicinal effect of one spice taken at a time on the body methodically and found that when these spices are taken in a specidied quantity, at a particular time with attention given to how they are taken, it can have wondrous effects on the health of an individual. Example cow ghee. It is a super barrier against all forms of bacteria, for the gums and the intestines. Mustard oil is for the sinus what blood is for the body, the ultimate protector. Complicated management of dust allergy is manageable in the simplest of ways by getting mustard oil to trickle in the sinovial cavities. Clove is the master rejuvenator, the antipyretic. When you feel run down, clove puts you back into action. Cardamom the body to retain more moisture in the intestines. That is good bye to hard stools. Fennel seeds is the natural brush for the intestines. Like a tooth brush, fennel also needs to be used judiciously. Gum arabica / edible gum is the spice to look to when we need slowing down of flow of food through the intestines for ensuring better absorption of nutrients. Basically, giving power back to the body for healing ensures the problem gets solved without any side effects and from the root.
Anil Kumar Chintaluri
GITAM University, India
Title: Antimicrobial activity of essential oils
Time : 14:55-15:15
Biography:
Anil Kumar Chintaluri Completed M.Sc., (Microbiology) from Acharaya Nagarjuna University, Guntur and M.Phil., from Andhra University, Visakahpatnam. Presently working as Sr. Asst. Professor, Dept. of Microbiology, Vizianagaram and Pursuing Ph.D from GITAM University, Visakhapatnam. I have Published 4 research papers in reputed International Journals. My area of research is on Antimicrobial activities of essential oils and Plant extracts & TLC – Bioautography.
Abstract:
Antimicrobial activity of essential oils.
Sandeep Arora
Chitkara University, India
Title: Novel bioactive semi-synthetic nitric-oxide releasing derivatives of resveratrol
Time : 15:15-15:35
Biography:
Will be uploaded soon.
Abstract:
Nitric Oxide is involved in a multitude of physiological and pathophysiological states in mammals. For example, it is now known to be involved in the control of blood pressure neurotransmission, and in the immune defense system of the body. Nitric Oxide has low solubility in water and is unstable in the presence of various oxidants. It makes it difficult to introduce as such into biological systems in a controlled or specific fashion. Therefore, development of chemical agents that release NO is important. Numerous compounds are available which show anti-inflammatory, analgesic, anti-ulcer, antimicrobial agents and antihyperlipidemic activity. Resveratrol is a natural compound reported to possess a broad spectrum of biological activity with less side effects. Due to its wide range of biological activity, resveraterol has received a considerable interest in the field of drug discovery and therefore it constitutes a relevant synthetic target in Pharmaceutical Industry. A series of resveratrol derivatives were schemed by treating with chloroalkanols or chloroacetic acid then with Nitric oxide donor group for nitrate introduction by treating alkylated compounds with a mixture of fuming nitric acid and acetic anhydride in chloroform in cold conditions to get the final compounds. The purity of the compounds ascertained by TLC in different solvent systems and the structures of the synthesized compounds confirmed by their elemental analyses, proton NMR, IR and mass spectral data. All the title compounds further put in process for screening for biological activity.
Anoja Megalathan
University of Sri Jayawardenepura, Sri Lanka
Title: Natural Turmeric enapsulated Layered Double Hydroxides as Anti-microbial Nanohybrids
Time : 15:35-15:55
Biography:
Anoja Megalathan has completed her under graduate degree at the age of 24 from Institute of Chemistry, Ceylon, Sri Lanka. She has a business start up on natural and healthy beverage company named ‘Aqua Sizzle’ and also worked as research scientist at Midaya Ceramics, Sri Lanka; research intern at the national University of Sri Jayawardenepura, Sri Lanka
Abstract:
Turmeric (Curcuma longa) has been long recognized for its anti-inflammatory properties against a wide variety of conditions. However, it’s practical applications have been limited due to the instability under thermal and UV radiation. There had been only few attempts to stabilize turmeric. There-fore, this study focuses on exploring the potential of nanotechnology based approaches to stabilize the natural turmeric and to evaluate the antimicrobial activity of the resulting nanohybrids. In this study, natural turmeric has been encapsulated into nano layers present in Mg-Al-Layered double hydroxides (LDHs) using a simple co-precipitation method. The suggested method avoids any isolation of active compounds, i.e. curcumin from natural turmeric. The LDHs selectively encapsulate curcumin during the synthesis process. Powder X-ray diffraction (PXRD) and Fourier transform infrared (FTIR) characterization confirmed the successful synthesis and encapsulation of curcumin into LDH. Thermogravimetric data and UV stability data confirmed the stabilization of the curcumin molecules within the LDH layers. Drug release characteristics of the nanohybrid was quantitatively monitored under different pH (3 & 5) conditions using UV-Visible absorption spectroscopy. Finally, the antimicrobial activity of turmeric-LDH nanohybrid was tested against three bacterial species including Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 25853) and two fungal species such as Candida albicans (ATCC 10231) and Candida dubliniensis (clinical isolate) using agar well diffusion method. The results show that the drug release of turmeric encapsulated LDH was a slow process, suggesting that the intercalated nanohybrid can be used as an efficient drug formulation.
Arjun Singh
Central Council for Research in Ayurvedic Sciences, India
Title: Comparative phytochemical study of heartwood versus small branches of acacia catechu using high performance thin layer chromatography
Biography:
Arjun Singh is working as Research Officer (Chemistry) in Central Council for research in Ayurvedic Sciences (CCRAS), Ministry of AYUSH, Govt. of India, New Delhi-58. He has 81/2 years experience in Research&Development, Standardization, Quality Control and Analysis of Drugs & Pharmaceuticals, Ayurvedic drugs & plant based medicines. He has also been worked (about 9 Years) in Central Revenue Control Laboratories, Dept. of Revenue (CBEC), Min. of Finance, Govt. of India and carry out Chemical analysis(using conventional & instrumental methods) of samples of various commodities for Revenue (Excise & Custom duty)purpose with a view to Export/Import / Excise Tariff act & policy. Presently he is engaged in various activities viz. Prepared SOPs and protocol for Standardization of Herbal drug and pharmaceutical, Pharmaceutical development of AYUSH CODED DRUGS and double blind randomized coding for clinical trial, Preparation of know how documents of drugs for patent filing, Technology transfer & commercialization. Prepare Drug Dossier & Drug Profile of Ayurvedic Drugs for preclinical/ clinical studies, Evaluation of EMR & IMR projects etc. He has published more than 20 papers, 7 Books / Mongraph(contributor), 3 Patent filed and 4 projects completed and also serving as an editorial board member of JDRAS.
Abstract:
To compare the phyto-constituents present in the heartwood and small branches of Acacia catechu on the basis of high performance thin layer chromatography in order to evaluate whether the small branches of this plant may be substituted with the heartwood. Acacia catechu (Family- Mimosaceae) commonly called Khadira is a medicinal plant widely used in Ayurveda. As per the Ayurvedic literature, heartwood of this plant is used in sáotha, Kustha, Prameha, Vrana. It is difficult to get huge amount of heartwood from this big tree without cutting the plant. Due to which availability of this plant may be difficult in near future for use in Indian system of medicine. Methods: CAMAG HPTLC system equipped with semi-automatic applicator Linomat-IV and win CATS 1.4.2 software was used. n-hexane, ethyl acetate and ethanolic extracts of the heartwood and small braches were developed in suitable mobile phase using standard procedures and visualized in UV 254 and 366 nm and in white light after derivatization with in Anisaldehyde-Sulphuric acid reagent. Results: The HPTLC fingerprinting of the n-hexane, ethyl acetate and ethanolic extracts of heartwood and small branches showed almost phytochemical profile. Conclusion: HPTLC fingerprint profile of heartwood and small branches are found similar, therefore small branches may be used in place of heartwood and vice-versa after comparison and confirmation of same for pharmacological activities. The results of qualitative evaluation of HPTLC fingerprint profile will also be helpful in the identification and quality control of the drug and can provide standard HPTLC fingerprints with selected solvent system. The method can also be used for identification of different Acacia catechu species and adulterants.
Ciddi Veeresham
Kakatiya Univeristy, India
Title: Development and Evaluation of selected Indian medicinal plant based poly herbal Anti-Aging cream
Biography:
Will be uploaded soon.
Abstract:
Practice of using herbal remedies as a strategy against aging skin has been evolved as an imperative step to accomplish comprehensive skin rejuvenation. Present study is aimed to prepare and evaluate a poly herbal cream containing standardized extracts of Aloe vera (A), Ocimum sanctum (O) and Punica granatum peel (P) at different ratios. Santalum album, Citrus aurantium, Olea europa, Prunus amygdalus oils, Cetyl alcohol and Stearic acid were used in a ratio of 0.1:0.1:1.0:0.5:0.5 and 3.0 respectively, along with preservatives to formulate a cream base for formulation water in oil (W/O) creams i.e. F1 to F6. Prepared formulations were evaluated for formulation parameters like pH, viscosity, spreadabilty, extrudability and stability. Further, formulations were also evaluated for anti-oxidant, anti-microbial and advanced glycation end product inhibition (AGE) properties. Formulations F3-F6 have shown no phase separation upon storage. Formualtions F3 and F6 have featured lower spreadability and extrudabiltiy. The order of anti-oxidant ability of the all formulations found as F6>F5>F4>F3>F2>F1. Formulations F4 and F5 shown comparable AGE inhibitory and anti-microbial effects to standards Aminoguanidine and Neomycin sulphate cream, respectively, however F4 shown higher AGE inhibitory effect vis-a-vis with F5. In human volunteer’s study, the formulations F4 and F5 shows no redness, edema, inflammation and irritation during irritancy studies. The synergistic effects of formulation F4 containing A: O: P (each 1.6% w/w) has addressed hyperpigmentation, erythema, wrinkles and firmness of skin more significantly than F5. These finding indicate the significant anti-aging potential of examined poly herbal anti aging cream.
Bhushan P Pimple
P. E. Society’s Modern College of Pharmacy, India
Title: Concomitant Management of Type-II Diabetes and its Associated Hyperlipidemia: Role of ethyl 2-[5-(acetyloxy)pently] benzoate
Biography:
Bhushan P. Pimple has completed his PG from University of Pune. He is working as Head, Department of Pharmacognosy, in PES Modern College of Pharmacy, Nigdi, Pune 411044 from last 10 years. He has published more than 6 papers in reputed international journals and has been serving as an reviewer to six international journals
Abstract:
Diabetes mellitus is a metabolic disorder prevailing throughout the world irrespective of age, sex and race. Moreover, complications associated with chronic diabetic conditions are severe and fatal. Normally, the patient exhibits cardiovascular disturbances, nephrological and neurological disorders. Objective: The present study focuses on management of type-II diabetes and associated hyperlipidemia with the use of ethyl 2-[5-(acetyloxy)pently] benzoate isolated from O. majorana leaves. Method: Streptozotocin, STZ (65 mg/Kg, i.p.) along with nicotinamide (120 mg/kg, i.p.) was used to induce type- II diabetes mellitus (NIDDM) in rats. Lipid profile was estimated after every 7 days for a period of 28 days. The rats were treated with various doses of ethyl 2-[5-(acetyloxy)pently] benzoate. The antidiabetic effect was compared with pioglitazone and antihyperlipidemic effect with Mevastatin. Results: Ethyl 2-[5-(acetyloxy)pently] benzoate significantly lowered the FBG in vivo and also elevated glucose absorption in isolated rat hemidiphragms. Our studies also revealed that the antihyperlipidemic property of ethyl 2-[5-(acetyloxy)pently] benzoate was comparable to Mevastatin. Furthermore, software assisted docking also proved the effective binding of ethyl 2-[5-(acetyloxy)pently] benzoate with PPAR-γ and HMG-coA reductase. Conclusion: Ethyl 2-[5-(acetyloxy)pently] benzoate can serve as a better candidate in the concomitant management of diabetes and its associated complications such as hyperlipidemia due to its versaltile nature.
- Track 1: Advanced Trends for the Future of Herbal Drugs and Products
Track 3: Herbal Drugs and Formulations
Track 9: Natural Products of Medicinal Interest
Young Research Forum
Location: Hyderabad International Convention Centre, India
Session Introduction
Meenakshi Barua
Birla College of Arts, Science and Commerce, India
Title: Physico-chemical, pharmacognostical evaluation and cytotoxicity study of cassia angustifolia (vahl) leaves
Time : 09:45-10:00
Biography:
Meenakshi Barua has completed her Graduation in Environmental Science from Delhi University (2007) and Mastered in Biotechnology from KIIT University, Bhubaneshwar (2010). During her post-graduation she completed her Dissertation in Environmental and Industrial Biotechnology Division at TERI, New Delhi (Jan – Jul 2010). She qualified GATE, (Biotechnology) in 2011. At present she is pursuing her PhD in Biotechnology from Birla College of Arts, Science and Commerce, Kalyan (University of Mumbai). She is a visiting faculty in the college in the subject of Biotechnology and presented papers (Oral and Posters) in national and international conferences.
Abstract:
Cassia angustifolia Vahl (Family – Caesalpiniaceae) commonly known as ‘Senna’ is one of the medicinally important, drought-resistant small perennial shrub. It is used as a febrifuge in splenic enlargements, anemia, typhoid, cholera and is an excellent blood purifier. It is also a rich source of polyphenols, glycosides, flavonoids, steroids and anthracene derivatives. Study of phytochemical and pharmacological features of senna leaves is of keen interest due to its huge medicinal properties. In the present study, leaves of Cassia angustifolia (Vahl) were used for its Physico-chemical and Pharmacognostical evaluation. Futher Cytotoxicity bio-lethality assay was performed on leaves using two different extracts (aqueous and hydro-alcohol) to check its toxicity. In Physico-chemical parameters, total moisture content (0.87 + 0.02%), total ash (10.2 + 0.05%), acid insoluble ash (0.85 + 0.002%), water soluble ash (9.8 + 0.04 %), sulphated ash (11.46 + 0.004%), alcohol soluble (5.84 + 0.72%) and water soluble extractive value (17.28 + 0.01%) were studied. Heavy metal (mercury, arsenic, lead, chromium and cadmium) analysis was found to be less than 0.01ppm i.e. in permissible limit. Zinc, an essential micronutrient for human being, was also found in permissible limit (14.72ppm) in the leaves. In phytochemical screening, xanthoproteins,carbohydrates, tannins, alkaloids showed maximum presence in acetone, methonal and aqueous extracts. Total alkaloids (119mg/g) and flavonoids(160mg/g) were determined using standard methods. In Cyto-toxicity bioassay, mortality rate was found higher in aqueous extract (67%) than in the extract of hydro-alcohol (20%). Analysis of all these parameters may significantly help in its use for the formulation of new pharmaceutical drug.
Khwaja Amtul Raouf Qazi
MRM College of Pharmacy, India
Title: Green factory kite as a natural substitute to heme
Time : 10:00-10:15
Biography:
Khwaja Amtul Raouf Qazi has completed her MPharmacy (Pharmacology) from Jawaharlal Nehru Technological University, Hyderabad, with an aggregate of First Class with Distinction.
Abstract:
Hemoglobin is a vital blood component. Heme in hemoglobin is present in hydrophobic pockets. It is structurally similar to chlorophyll with a difference in Mg-centered porphyrin ring in chlorophyll and Fe-centered porphyrin ring in hemoglobin. The high percentage of chlorophyll content in a natural form as a grass juice is preferred in clinical conditions involving hemoglobin deficiencies. Green vegetation (chlorophyll) are a factorial area to produce porphyrin ring. This porphyrin ring resembles “Kite” because of its structural porphyrin-head & phytol-tail. Chlorophyll obtained undergoes a catabolic process and could have physiological roles. The Fe-Centered porphyrin ring obtained will act as a natural substitute in hemoglobin deficiency conditions. Iron remains in the ferrous (Fe++) state in both oxygenated and deoxygenated hemoglobin. While in met-Hb, ferritin, transferin iron is in ferric (Fe+++) form. The oxygenation of one heme molecule enhances and accelerates oxygenation of other heme molecules. Similarly, the release of one oxygen molecule promotes the release of other which is required to meet metabolic demand of blood. 3.34 mg of iron-centered porphyrin ring of chlorophyll may require maximally bind of 1.34 ml of oxygen to form 1 gm of hemoglobin. Thus, the main aim is to provide beneficial effect of Fe-centered porphyrin ring of chlorophyll as a natural substitute to heme. The result of study may help the hemoglobin deficient patients to meet their iron and health needs.
Behzad Mohaddesi
Saurashtra University, India
Title: Phytochemical evaluation and anti cancer screening of cardiospermum halicacabum Seeds extracts on different Human Cancer Cell Lines
Time : 10:15-10:30
Biography:
Behzad Mohaddesi has completed his M.Sc on Medicinal Plants Sciences from Gujarat Ayurved University, India. He is pursuing his Ph.D. degree on herbal drug technology in Department of Pharmaceutical Sciences, Saurashtar University, Faculty of Pharmacy. His area of work is on traditional medicine, ethno pharmacology and evaluation of biological activity of medicinal plants.
Abstract:
In modern life the growth of different acute and chronic disease increased and cancer is one of the disorders with significant prevalence rate among them. As knowledge has increased about the traditional medicine, the daily interest to use of natural products especially herbal drugs are becoming more from patients to physicians with aim to prevention of disease and safer way of treatment with lesser side effect for such conditions. Hence, this study undertaken to evaluate the effect of cardiospermum halicacabum a traditional medical plant. Cardiospermum halicacabum Linn. from Sapindaceae family, known as love in a puff in English and karṇasphoá¹a in Hindi. Different part of this plant such as leaves, fruits and seeds are traditionally used for treatment of various diseases in different system of medicine such as Ayurveda, Chinese, Homeopathy and Unani. The seeds of plant selected for this study and subjected to soxhlet extraction to obtain the various extracts, preliminary phytochemical studies performed for each extract and individually tested against four human cancer cell lines, HT-29, HCT-15, SK-MEL-2 and MCF7 with Sulforhodamine B colorimetric (SRB) cytotoxicity assay and compared with Doxorubicin a known anti cancer drug. The most active extract selected and subjected to phytochemical analysis by GC-MS to find out the probable active components. Result of this work indicated the potential medicinal value of cardiospermum halicacabum seeds as anti-neoplastic agents on human cancer cell and the GS-MS analysis showed the present of different fatty acids in the seed extract which can be responsible for the anti cancers activity.
Hridya Hemachandran
VIT University, India
Title: Evaluation of Pterocarpus santalinus Linn. F. methanolic extract as a natural melanogenesis inhibitor: In vitro study in B16F0 melanoma cell lines
Time : 10:30-10:45
Biography:
Will be uploaded soon
Abstract:
Melanin, the end product of melanogenesis determines the skin, hair and eye colour. Excessive production of melanin causes hyperpigmentation disorders which results in serious aesthetic problems. Pterocarpus santalinus also known as red sanders or raktha chandan is a red dye yielding tree commonly used in treatment of skin disorders and insect bites in Indian traditional medicine. Here, we investigate the anti-melanogenesis effect of methanolic extract of Pterocarpus santalinus by cell free tyrosinase assay, followed by cell viability, cellular tyrosinase and melanin content assay in B16F0 melanoma cell lines. The heart wood of Pterocarpus santalinus was extracted in methanol and the skin whitening ability was tested. It has been observed that methanolic extract of red sandal wood shows dose dependent inhibitory activity on cell free and cellular tyrosinase activity. No cytotoxic effect was observed in cells treated with the extract upto 200 µg/ml concentration. Decreased melanin pigmentation was also observed in cells treated with Pterocarpus santalinus methanolic extract. Our finding exhibit skin whitening ability of Pterocarpus santalinus methanolic extract and serves its use in treatment of several hyperpigmentation disorders and also as a skin whitening agent in cosmetic industry replacing synthetic toxic skin whitening agents.
Nabanita Chakraborty
ICAR- Central Inland Fisheries Research Institute, India
Title: Wet-land colonizers to anti-dandruff dermatitis: Study with swamp cabbage
Time : 10:45-11:00
Biography:
N Chakraborty is pursuing her PhD from Central Inland Fisheries Research Institute (CIFRI), Indian Council of Agricultural research under DST Women Scientist Fellowship (WOS-A). She published 7 papers in international journals and one book chapter.
Abstract:
The pharmaceutical evaluation of the aquatic macrophytes is an emerging aspect of allelochemistry. In this context, swamp cabbage (Ipomea aquatic Forssk; Convolvulaceae) a semiaquatic, tropical plant grown as a vegetable and a key ecological community in wetland ecosystem has been focussed. Our current study aims for microbial screening of Ipomea leaves against facultative anaerobic bacteria to catfish, Edwardsiella tarda and fungal dermatitis Malassezia globosa. Extraction of crude leaf extract followed by liquid-liquid extraction process and further crystallized and each fraction subjected to biochemical analysis viz. phenols, flavonoids and tannins followed by selective antioxidant and antimicrobial activity. The inductive inferences of the various assays showed the ethanol fraction (80%) of the plant to be a natural resource to antidandruff compounds against M. globosa and ethyl acetate fraction to be preventive against E.tarda. BLA has also been conducted to address preliminary cytotoxicity issues as water being a non-resource variable and the lethal concentration was found to be considerably safe. A statistical approach to dose response curve is attempted for prediction of desired doses and validating the results.
Snehal Bhavsar
North Maharshtra University, India
Title: Design, development and characterization of naringenin loaded solid lipid nanoparticles for oral delivery
Time : 11:15-11:30
Biography:
S K Bhavsar has completed MPharm (Pharmacognosy) from R C Patel Institute of Pharmaceutical Education and Research, Shirpur North Maharashtra University, Jalgaon. She was University Topper and Gold Medalist in MPharm and is now pursuing PhD from the same institute.
Abstract:
Naringenin (NAR) is highly lipophilic drug having log P value 2.5. The poor orally available NAR only 8% oral bioavailability was successfully encapsulated in compritol based Solid Lipid Nanoparticles (NAR-SLNs) for its ultimate use to target intestinal lymphatic transport system was prepared. Result showed that SLNs with mean particle size of 66.56 nm (polydispersity index, PDI=0.31) and surface electrical charge of approx. −12 mV, were produced by high pressure homogenization process. Response Surface Methodology (RSM) using the Central Composite Rotatable Design (CCRD) model was used to optimize formulations of NAR-SLNs. Particles were characterized using Differential Scanning Calorimetry (DSC), XRD and Scanning Electron Microscopy (SEM) to confirm their solid character and the homogeneous distribution of drug within the lipid matrix. In vitro release studies at pH 6.8 phosphate buffer (PBS) and % cumulative drug release of 94.97 at the end of 24 h. The accelerated stability studies showed that there was no significant change in the mean particle size and PDI after storage at 25±2 ËšC/60±5% RH for the period of three months. This study showed that the RSM–CCRD could efficiently be applied for the modeling of NAR-SLNs.
Xiu-Qiong Fu
Hong Kong Baptist University, China
Title: Involvement of TLR4/STAT3 signaling in the anti-melanoma effects of atractylenolide II
Time : 11:30-11:45
Biography:
Xiu-Qiong Fu has completed her MPhil at Southern Medical University in Guangzhou, China. Now, she is a PhD candidate of Hong Kong Baptist University. She has published more than ten papers in reputed journals. Her relevant research work is pharmacology of Chinese medicines.
Abstract:
Melanoma incidence and mortality rates are rapidly increasing. STAT3 (Signal Transducer and Activator of Transcription 3) and TLR4 (Toll-Like Receptor 4) have been shown to be activated in melanoma cells. Suppressing TLR4 or STAT3 signaling in tumor cells represents an antimelanoma strategy. Rhizoma Atractylodis Macrocephalae (Baizhu in Chinese), a Qi-tonifying Chinese medicinal herb, is commonly prescribed by traditional Chinese medicine doctors for treating melanoma. We have demonstrated that atractylenolide II (AT-II), isolated from Baizhu, can significantly inhibit B16 tumor growth at non-toxic doses in mice, which is associated with a potent suppression of STAT3 signaling. We have also found that AT-II inhibits melanoma metastasis in mice and cell migration in A375 and B16-F10 cells. Molecular docking indicated that AT-II could bind to TLR4/MD-2 receptor complex. MPLAs, specific TLR4 agonists, significantly diminish AT-II-afforded suppression on STAT3 phosphorylation/activation and melanoma cell migration. Based on these data, we concluded that inhibiting the TLR4/STAT3 pathway is involved in the anti-metastasis effects of AT-II. This study advances our understanding of the anti-melanoma mechanisms of AT-II, and provides chemical and pharmacological justifications for the clinical application of Baizhu in melanoma treatment.
Sonali Mohanty
S.S Institute of Medical Sciences & Research Centre, India
Title: Evaluation of anticonvulsant activity of Turnera diffusa in Albino Mice.
Time : 11:45-12:00
Biography:
Sonali Mohanty, completed MBBS in 2011 from Bharati Vidyapeeth Deemed University, Pune.Currently pursuing Post Graduation in M.D. Pharmacology at SSIMS&RC, Davangere. KMC
Abstract:
Turnera diffusa commonly known as Damiana has a long history of use in traditional herbal medicine throughout the world. The medicinal part is its leaves, which are harvested during the flowering season. Damiana is known to act as an aphrodisiac, antianxiety, cough-suppressant, analgesic etc.The present study is done to evaluate the anticonvulsant activity of Turnera diffusa in albino mice.For the same purpose a total of 60 animals were divided into 10 groups of 6 each. Among which 5 groups were subjected to Maximal Electroshock Seizure(MES) model and other 5 groups to Pentylenetetrazole(PTZ) induced seizure model. Normal Saline was given as control. Sodium valproate 50mg/kg body weight per orally was used as standard. Aqueous extract of T. diffusa was used in three doses 50, 75, 100 mg/kg body weight per orally. In MES model there was a marked reduction in duration of all the parameters (tonic hind limb flexion, tonic hind limb extension, clonus and postictal depression) and was significant when compared with control(p<0.001) and was comparable with standard.In PTZ induced seizures prolongation of duration of onset of seizures and decrease in duration of seizures were observed. When compared to control, results were significant (p<0.001) and were comparable with standard .This study showed that T.diffusa has anticonvulsant activity.Further research is required to explore the utility of damiana for treatment of epilepsy.
Nayana Matada Revanasiddaiah
S.S Institute of Medical Sciences & Research Centre, India
Title: Evaluation of antidepressant activity of Turnera diffusa in albino mice
Time : 12:00-12:15
Biography:
Nayana M.R,completed MBBS in 2012 from RGUHS. Now pursuing postgraduation in M.D. Pharmacology in SSIMS, RGUHS. KMC
Abstract:
Turnera diffusa commonly known as Damiana is used for its medicinal property. Medicinal plant is its leaves which are harvested during flowering season. In traditional medicine it is used as aphrodisiac, antidepressant, anti-anxiety, gastro-protective etc. In present investigation, aqueous extract of Turnera diffusa was evaluated for its antidepressant activity in Albino mice. Turnera diffusa was subjected to antidepressant activity evaluation at various doses that is, 50, 75, 125 mg/kg p.o, Amitriptyline(20mg/kg) was used as standard. All the drugs were given 30 min before the test to mice. Rodent models of depression such as Forced swimming test(FST) and Tail suspension test(TST) were used for study. Turnera diffusa exhibited significant antidepressant activity at 50, 75 mg/kg body weight respectively with reference to control and as well as comparable with standard. At a dose of 125mg/kg it showed significant antidepressant activity compared to standard. In FST and TST there was a significant reduction in immobility period in animals treated with T.diffusa compared to control group (p<0.001) and immobility period was comparable with that of standard. The present study showed that T.diffusa has antidepressant activity and may be used as an alternative or add on therapy for treatment of depression.
Divya Reddy
JSS Medical College, India
Title: Study of the diuretic activity in aqueous extract of roots of Anacyclus Pyrethrum in albino wistar rats
Time : 12:15-12:30
Biography:
Divya Reddy K, completed my MBBS from Mahadevappa Rampure Medical College, Gulbarga. Presently I am doing my post-graduation course in Pharmacology at JSS medical college, Mysuru. Karnataka as .My dissertation is on herbal plant for its anticancer activity on lung cancer cell lines. (Invitro study).I presented a poster presentation at carcinogenesis 2015-Mumbai on EAC cell lines .I have 2 publications.
Abstract:
The aim of the study is to evaluate the diuretic activity of aqueous extract of roots of Anacyclus pyrethrum. Albino wistar rats weighing 200-250 gms of either sex were randomly selected from Central animal facility, JSS Medical College Mysuru. They were divided into 3 groups of 6 rats each (n=6).The animals were fasted overnight. Next day group 1which is control-received normal saline (25ml/kg.p.o), Group 2 which is standard-Furosemide 20mg/kg body weight. Group 3 which is test received 100mg/p.o Aqueous extract of roots of Anacyclus Pyrethrum. The experiment was carried out in a diuretic cage. The results, were analyzed by one Way Analysis Of Variance (ANOVA) .There was a significant diuretic activity compared to that of control. P value of less than 0.05 was considered significant.There was significant increase in urine volume by the aqueous extract of roots of Anacyclus Pyrethrum when compared to the control group.While the excretion of Urinary electrolyte concentration revealed a diluted urine in the test group signifying the diuretic activity of the extract. Aqueous extract of Anacylus pyrethrum have a significant diuretic activity in Albino wistar rats. Further tests are to isolate the active principles responsible for the activity suggested & also required to evaluate diuretic property in human subjects.
Raman Rajeshkumar
JSS College Of Pharmacy, India
Title: Alterations in mitochondrial apoptosis regulating gene expression in human cervical cancer cell line using a new compound isolated from the bark of Symplocos cochinchinensis (Lour.) S. Moore
Time : 12:30-12:45
Biography:
Raman Rajeshkumar has completed his M Pharm from JSS College of Pharmacy, Ooty and doing his PhD at JSS University, Mysore. At present he is working as a lecturer, Department of Pharmaceutical Biotechnology at JSS College of Pharmacy, Ooty since last 8 years. His field of research work is mainly in Free Radical Biology and Cancer Biology.
Abstract:
Mitochondria have been reported that cancer cells exhibited aerobic-glycolysis. Although this was originally construed as suggesting that the function of mitochondria was defective, we instantly realize that cancer cells are in an altered metabolic state. Little care has been compensated to the potential mutations that can affect mitochondrial function in cancer, outside of specific variations in genes. We were reporting that the new compound isolated from Symplocos cochinchinensis (Lour.) S. Moore had stimulated the expression of a few genes in mitochondria in human cervical cancer cell line. The Hela cells were treated with EA1 with their IC50 concentrations obtained from cytotoxicity. Various incubation duration, such as 0h, 24h, 48h and 72h were selected for the study. To elucidate the apoptotic pathways activated by EA1, mitochondrial transmembrane potential loss, Semi quantitative RT PCR and Western Blot analyses were carried out to measure the expression of death receptor as well as their apoptotic genes and bcl-2 family genes. Analysis of these genes revealed a substantial reduction in the expression of bcl-2 and cox-2 and increase in the expression of Bax and p53 was observed. Cells treated with EA1 was compared with untreated control cells. The ß - actin gene was used as an internal house keeping control for the study. In the present study, we noticed that the EA1 time dependently regulated the expression of Cox-2. Our works also demonstrate that the initiation of apoptosis was closely connected with reduction in mitochondrial transmembrane potential.
Pradeep B
JJM Medical college, India
Title: A review on the potential therapeutic profile of carica papaya leaf extract in the management of dengue fever
Time : 12:45-13:00
Biography:
Pradeep has completed his MBBS at the age of 25 years from JJM Medical College, Davangere, under Rajiv Gandhi University of Health Sciences, Bangalore and is currently doing post-graduation from the same academic institution. Currently he is actively involved in many research activities as his academic interest.
Abstract:
Dengue is an acute viral infection with potential fatal complications. Dengue virus (DV) belongs to the family Flaviviridae which has four serotypes (DV-1 to 4). One of the most disturbing aspects of the problem of dengue is that there are no effective antiviral agents to treat dengue complications. Treatment for dengue is mainly symptomatic. Some cases require platelet transfusions and fluid management. Papaya, Carica papaya known in Ayurveda as Erand-karkati, has many benefits linked to other parts of this plant, as its leaves. Various clinical and preclinical studies have demonstrated a positive effect of its leaf extract in dengue cases with thrombocytopenia. It induces increase in platelet count within 2 days of first dose which is very much evident at the end of 5th day. It prevents Dengue Fever worsening into Dengue Hemorrhagic Fever and thus promotes prompt recovery, avoiding hospitalization. It provides additional benefits in patients with Dengue fever which include increase in the WBC count, improvement of liver functions, and normalization of clotting profile. Few adverse events reported, related to GI disturbances like nausea, vomiting with commercially available preparations of Carica papaya leaf extract (Tab. Caripill) with no effects related to drugs tolerability and safety. To conclude, the Carica papaya leaf extract provides an overall benefit in dengue cases and there is more scope for its use in the coming days as there is obvious evidence of its efficacy and safety and because there are no specific treatment options available, except the symptomatic approach.
Shashikala G
JJM Medical college, India
Title: Evaluation of effect of alcoholic extract of Tinospora Cardifolia on learning and memory in Alprazolam induced amnesia in albino mice
Time : 13:30-13:45
Biography:
Shashikala.G has completed MBBS at the age of 22years from J.J.M.Medical College, Davngere, Rajiv Gandhi University of Health Sciences, Bangalore. Currently postgraduate in M.D. PHARMACOLOGY from the same academic institution and involved in various curricular activities.
Abstract:
Learning is defined as the acquisition of information and skills, and subsequent retention of that information is called memory. Learning and memory can be a psychological process as well as a change in synaptic neural connectivity. Memory is the most common cognitive ability lost with dementia. The study was undertaken to evaluate the learning & memory effect of Alcoholic extract of Tinospora cardifolia in alprazolam induced amnesic mice.The study was carried out on albino mice, divided into 4 groups of 6 animals each (3-4months of age, weight 25-30g). Amnesia was induced by administering Alprazolam (2mg/kg body wt for 14 days) in all 4 groups for 14days from 1st to 14th day.Group 1 would be given memory impairing dose of Alprazolam (2mg/kg/p.o) alone for 14days. Group 2 would be given Piracetam (400mg/kg p.o) from 8th to 15th day. Group 3 would be given Alcoholic extract of Tinospora cordifolia 140 mg/kg, p.o. from 1st to 15th day. Group 4 would be given Alcoholic extract of Tinospora cordifolia 280 mg/kg, p.o. from 1st to 15th day.The learning and memory of the animals was assessed by employing Elevated plus maze [transfer latency (TL)] & Step-down type passive avoidance model[step down latency (SDL)]. The measured parameters TL & SDL were compared with standard drug Piracetam. In both EPM & SDA model results were comparable with the standard. Tinospora cardifolia, Indian medicinal plant useful for treatment of various ailments like Diabetes,Rheumatoid arthritis,Gout,Viral hepatitis,Gonorrhoea,Syphilis and Anemia can also be a useful alternative for treating Dementia and associated diseases like Alzheimer’s disease.
Deepa Halemani
JJM Medical college, India
Title: Evaluation of antidepressant effect of methanol extract of Aegle Marmelos (Bael fruit tree) leaves in mice
Time : 13:45-14:00
Biography:
Deepa halemani is currently pursuing his 2nd year post graduate in Department of pharmacology at J.J.M Medical college, India
Abstract:
Depression is one of the most common mental disorders, characterised by sad mood, loss of interest and pleasure, worthlessness and suicidal thoughts. Currently Tricyclic antidepressants and SSRIs are most commonly employed drugs for the treatment of depression. Synthetic drugs available for treatment of depression have various adverse effects like sedation, dry mouth, postural hypotension and tachycardia etc. Drugs obtained from natural sources are known to cause fewer side effects compared to synthetic drugs despite of same ability to cure disease. Therefore this study is undertaken to evaluate the antidepressant property of methanol extract of Aegle marmelos (AM) (an ayurvedic medicinal tree, commonly known as the bael fruit tree) leaves in mice. Methanol extract of Aegle marmelos (100mg/kg, 200mg/kg and 400mg/kg), 2% of Gum acacia (control), Imipramine 20mg/kg (standard) are administered orally to randomly divided albino mice of either sex. Antidepressant activity is assessed by Tail suspension test (TST) and Actophotometer (locomotor activity) models. In TST and Actophotometer models ANOVA with post hoc test was used to assess antidepressant activity between groups. AM of all doses (100mg/kg, 200mg/kg, 400mg/kg) have shown statistically significant decrease in immobility time (p<0.05) compared to control and results are comparable with standard in TST. In Actophotometer model AM in all the doses have shown statistically significant increase in locomotor activity (p<0.05) compared to control and results are comparable with standard.
Pavitra R Y
JJM Medical college, India
Title: Evaluation of antidepressant activity of aqueous extract of the Prosopis cineraria in albino mice
Time : 14:00-14:15
Biography:
Pavitra graduated MBBS from BLDE medical college, Karnataka. Now pursing postgraduation in Pharmacology from JJM medical college, Karnataka. I have attended various state and national conferences and presented poster in IPSCON 2013 and published few articles.
Abstract:
Depression is the most prevalent mental disorder. Basic neuroscience offers the promise of improving our understanding of disease pathophysiology, identifying novel mechanisms that can be targeted by more effective pharmacotherapies and screening of herbal sources of drugs. The leaves of Prosopis cineraria are selected for evaluating its antidepressant due to its traditional use in the management of various CNS disorder. Aim of the study: To evaluate the antidepressant activity of aqueous extract of the Prosopis cineraria in albino mice. Materials and methods: Albino mice -24, Plastic cylinder, Drugs-Imipramine 15mg/kg, Aqueous extract of Prosopis Cineraria [AEPC] 100mg/kg and 200mg/kg. A total of 24 Albino mice will be divided into four groups of 6 animals each using forced swimming method. Group I served as control which received distilled water. Animals of group II received standard drug Imipramine at a dose of 15mg/kg. Group III received the AEPC orally at a dose of 100 mg/ kg. Group IV received the AEPC orally at a dose of 200 mg/ kg. Drugs were dissolved in distilled water and given orally one hour before the experiment. Results: Analysis is done using ANOVA and Post hoc analysis. Immobility time decreased with increase in the dose of aqueous extract of Prosopis cineraria [AEPC] 200mg/kg > 100mg/kg with significant p value and is comparable with standard drug imipramine 15mg/kg.
Biography:
Will be uploaded soon.
Abstract:
The plants belonging toBerberis species are extensively used in various Ayurvedic, homeopathic and ethno-medicines as raw material or as an ingredient. Presence of various major and minor secondary metabolites and their amount plays very crucial role in the standardization and quality control of herbal medicines. Therefore, more objective and definitive methods are necessary for knowing about the chemical variability amongst the genuine and commonly available species of the same plant.In the present study an attempt has been made to critically analyse the variability among the popular Berberis species on the basis of conventional single marker based approach and subsequently using the more advanced method of HPLC fingerprinting with principle component analysis and similarity analysis. The experiments were carried out to throw light on the variation in extractive value (%) and berberine content (%) with species, location, part of the plant and season and use of comprehensive HPLC DAD profile with PCA to access the variability among different species and effect of location, and stage of harvested plants (vegetative and reproductive). It was concluded thatPCA models constructed as per the locations and species and similarity analysis done on the basis of comprehensive HPLC fingerprinting data could be very useful for the herbal industries for predicting the origin of raw material, variability study for the authentication and standardization of raw material in terms of location and species.
Chaitanya Sravanthi Kota
JNTU University, India
Title: Priliminary phytochemical investigation and pharmacological screening of caseariaellipticabark for antidiabetic activity
Time : 14:30-14:45
Biography:
Chaitanya sravanthi kota is pursuing her Phd at Jawaharlal Nehru Technological University, in the work titled “Phytochemical Investigation and Pharmacological Screening Of Selected Plants For Antidiabetic Activity and Associated Complications.” She is Associate Professor and head of Department of Pharmacognosy at Vignan Institute of Pharmaceutical sciences, an Pharmacy educational institute affiliated to Jawaharlal Nehru Technological University. Presented poster in “5th International conference on Medicinal Plants and Herbal Products”, at Manipal in 2013, and 66 th Indian Pharmaceutical Congress. She has Published about 12 articles in International journals. Attended conferences both National and International on GLP, Quality Control of Ayurveda medicine, Agriculture biotechnology.
Abstract:
The metabolic disorder which has become more common in world population is Diabetes mellitus. Ample number of formulations of medicines for Diabetes are available but still this disorder requires a search for a drug with no or considerablely less side effects. This thought gave scope for complementary therapies. A study on bark of Casearia elliptica was conducted for evaluation of antidiabetic activity in STZ induced diabetic rats. Rats of body weight 180-200gms were administered with STZ dose 60 mg/kg body weight to induce diabetes. Five rats were placed in each group . Rats were grouped into Normal group (Group I) ,Diabetic control treatedwith STZ (Group II), Diabetic rats treated with Glibenclamide (Group III), Diabetic rats treated with Casearia elliptica bark extract(Group IV). Group III is administered with 10mg/kg of standard drug Glibenclamide .The Caseariaelliptica extract were administered with doses of 200mg/kg & 400mg/kg using CMC solution (5%) as the vehicle. Blood glucose in Group IV,III,II, I, on 14th day were 164.4±1.29, 98.8 ±1.15, 291±0.71,107±1.14 respectively . Observation shows that standard is one and half times morepotent than Group IV in hypoglycemic effect at a dose of 200mg/kg.. Body weights of Group IV , III , II,I and on 14th day were198.22±1.23, 187.2±0.96, 165.8±1.2,193.5 ±1.5, and with no toxic effects were observed in the Test Group (Group IV). Body weights and blood glucose levels were compared with Group II. Proteins,Carbohydrates;secondary metabolites like Alkaloids, Steroids ,Saponins and Flavonoids were present in Casearia elliptica which were detected by preliminary chemical tests
Uzma Azeem
Punjabi University, India
Title: Evaluation of nutraceutical composition of Phellinus gilvus (Schw.: Fr.) Ryv.
Time : 14:45-15:00
Biography:
Uzma Azeem doing PhD under the guidance of Dr. Gurpaul Singh Dhingra, Professor, Department of Botany, Punjabi University, Patiala and co-supervision of Dr. Richa Shri Associate Professor, Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala.
Abstract:
Mushrooms have long been appreciated for their flavor, medicinal and nutraceutical attributes. They have been receiving much attention as good neutraceutical and pharmaceutical source because they are rich in various bioactive compounds. The genus Phellinus (family: Hymenochaetaceae) has been receiving special concern because of its potent nutritive and medicinal properties. There are various species of the genus Phellinus that are known to be used as a cure against various ailments like diarrhoea, stomachaches, inflammation, arithritis, hepatoprotection, detoxification, combating allergy and diabetes since ages. In the present investigation, the powdered basidiocarp of the mushroom Phellinus gilvus has been studied for its various neutraceutical properties in terms of its mineral constituents and fatty acid composition. The mineral composition of Phellinus gilvus has been determined by wavelength dispersive X-ray fluorescence (WDXRF) technique. The presence of various minor and major mineral nutrients having immense role in boosting human health have been detected. The fatty acids which are important nutrition and physiology components have been analyzed by GC-MS technique. The mushroom has been found rich in various nutritive and medicinally important saturated and unsaturated fatty acids. The present study revealed that the mushroom Phellinus gilvus can be used in neutraceutical and herbal drug formulations.
Pallavi M Kamath
JJM Medical college, India
Title: Evaluation of anti-convulsant activity of ethanolic extract of Murraya koenigii leaves in Wistar rats
Time : 15:15-15:30
Biography:
Pallavi M Kamath has completed MBBS from AJ Institute of Medical Sciences, Mangalore and is currently pursuing Post Graduate course in MD Pharmacology at JJM Medical College Davangere, Karnataka.
Abstract:
Murraya koenigii, commonly known as curry leaf, is a tropical-subtropical tree of family Rutacea which is native to India. The leaves of Murrya koenigii is commonly used as seasoning for curries. It is also used traditionally for the treatment of epilepsy. The aim of the present study is to evaluate the anticonvulsant activity of ethanolic extract of Murraya koenigii in wistar rats using pentylenetetrazole (PTZ) induced seizure model and maximal electroshock seizure (MES) model. Thirty adult rats of both sexes weighing 150-200g were equally distributed into five groups of six each and subjected to seizures induced by PTZ and MES. The ethanolic extract of Murraya koenigii 500mg/kg, 250mg/kg, 125mg/kg were studied for its anticonvulsant activity. The standard being Sodium Valproate 200mg/kg and Control being suspending agent- 1% tween 80 . All drugs were given orally one hour before the artificial induction of seizure. The test drug showed statistically significant result compared to control in both PTZ and MES models.
Arun Suresh
JJM Medical college, India
Title: Medicinal plants with skeletal muscle relaxant activity: A review
Time : 15:30-15:45
Biography:
Arun Suresh has completed MBBS from AJ Institute of Medical Sciences, Mangalore and is currently pursuing Post Graduate course in MD Pharmacology at JJM Medical College Davangere, Karnataka.
Abstract:
Nature provides us an abundant supply of plants with medicinal properties that can pave the way to innumerable drug discoveries. Skeletal muscle relaxants are used to treat muscle spasm and spasticity. Muscle spasm is a sudden involuntary contraction of one or more muscle groups and is usually an acute condition associated with muscle strain (partial tear of a muscle) or sprain (partial or complete rupture of a ligament). Skeletal muscle relaxants consist of both antispasticity and antispasmodic agents. This is a broad categorization and prescribers usually do not differentiate between them. The antispasticity agents like baclofen, tizanidine, dantrolene aid in improving muscle hypertonicity and involuntary jerks. Antispasmodic agents, such as cyclobenzaprine, are primarily used to treat musculoskeletal conditions. The side effects of antispasmodic agents and antispasticity agents cause them to be used with caution. Therefore there is a need to discover a newer alternative for skeletal muscle relaxants that has a safer profile. Including the prototype skeletal muscle relaxant Tubocurarine, most of the modern day skeletal muscle relaxants are directly or indirectly of plant origin. Hence there should be systematic search within the herbal products in an attempt to find a plausible replacement. In this review article, the data was compiled from the latest, original relevant research articles and aims to enumerate the medicinal plants that possess skeletal muscle relaxant activity, the bioactive extract involved, dosage used, animal models employed for assessment, active phytochemicals contained in them and their possible mechanisms of action.
Vipul Suryavanshi
Bombay College of Pharmacy, India
Title: Resazurin microtitre assay (REMA) for antibacterial and antifungal activity of herbs of three antidiarrhoeal formulations: Bilagyl® and Berbenterone® tablets and Berbenterone® suspension
Time : 15:45-16:00
Biography:
Krishnapriya Mohanraj (corresponding author) has completed her PhD (Tech) in Pharmaceutical Chemistry from Institute of Chemical Technology (ICT), Mumbai in 1998. She is Head of Department of Pharmaceutical Analysis at Bombay College of Pharmacy. Her expertise includes Computer aided drug design, synthesis and pharmacological evaluation, bioactivity guided fractionation of herbal extracts, enzymatic resolution, chiral chromatography using achiral and chiral columns, impurity profiling, analytical method development and validation, bioanalysis and structural elucidation
Abstract:
Bilagyl®, Berbenterone® tablets and Berbenterone® suspension are Ayurvedic formulations used for antidiarrhoeal therapy. Herbs present in these formulations [Aegle marmelos (Bilwaphal), Berberis aristata (Daruharida), Punica granatum (Dadimtvak), Holarrhena antidysenterica (Kutajchal), Syzygium aromaticum (Lavang), Myristica fragrans (Jaiphal), Quercus infectoria (Maiphal), Cyperus rotundus (Nagarmotha), Aconitum heterophyllum (Ativisha), Embelia ribes (Vidang)] were subjected to various extraction procedures and the extracts were investigated by Resazurin microtitre assay (REMA) against Salmonella typhi, Escherichia coli, Staphylococcus aureus, Shigella flexneri and Candida albicans. All plants investigated showed anti-infective activity but only certain extracts of the herbs investigated were active. Some extracts showed only antibacterial activity while some extracts showed both antibacterial and antifungal activity. Hexane extract of Embelia ribes (Vidang) showed lowest MIC values against E. coli (0.190-0.381 μg/ml), S. flexneri (0.190-0.381 μg/ml), S. aureus (0.190-0.381 μg/ml) and S. typhi (3-6 μg/ml). Chloroform extract of Myristica fragrans (Jaiphal) and Quercus infectoria (Maiphal) showed lowest MIC value against C. albicans (24.4-48.8 μg/ml). Ciprofloxacin [MIC values: E. coli (0.01-0.02 μg/ml), S. flexneri (0.04-0.08 μg/ml), S. aureus (0.08-0.16 μg/ml) and S. typhi (0.04-0.07 μg/ml)] and fluconazole (MIC against C. albicans is 4.8-9.7 μg/ml) were used as standard antibacterial and antifungal agents, respectively.
Damayanthi Dalu
Jntu university, India
Title: Antidiabetic, antihyperlipidemic and antioxidant properties of roots of ventilago maderaspatana gaertn. On streptozotocin-induced diabetic rats.
Time : 16:00-16:15
Biography:
Will be uploaded soon
Abstract:
The present investigation was aimed to evaluate the traditional claim of Ventilago maderaspatana (V.maderaspatana) in diabetes. Furthermore antihyperlipidemic and antioxidant properties were evaluated since hyperlipidemia and free radical damage leads to diabetes. Hence the present study was aimed to evaluate antidiabetic, antihyperlipidemic and antioxidant activity. Antidiabetic activity was evaluated by oral glucose tolerance test and streptozotocin-induced model. Antihyperlipidemic activity was evaluated by estimating lipid levels. In addition Ventilago maderaspatana was also evaluated for antioxidant activity employing catalase, lipid peroxidase and glutathione reductase methods. By soxhlet extraction process alcoholic, hydroalcoholic, chloroform and petroleum ether extracts were obtained. All these extracts except petroleum ether were evaluated for toxicity unto 3000 mg kg-1. In oral glucose tolerance test chloroform extract did not produce significant glucose lowering effect. Alcoholic and hydroalcoholic extracts of Ventilago maderaspatana elicited significant glucose tolerance effect. Hence VMAE and VMHAE were screened further by streptozocin induced diabetic model. VMAE and VMHAE significantly lowered blood glucose, triglycerides, total cholesterol, LDL cholesterol, VLDL cholesterol, creatinine, urea and increased HDL cholesterol, serum insulin and liver glycogen levels when compared to standard drug glibenclamide (10 mg kg-1). V.maderaspatana also increased catalase levels and decreased lipid peroxidase and glutathione reductase. VMAE and VMHAE elicited significant dose-dependent antidiabetic, antihyperlipidemic and antioxidant activity. VMHAE at 500 mg kg-1 induced more significant antidiabetic activity than VMAE (500 mg kg-1). VMAE at 500 mg kg-1 elicited more antihyperlipidemic and antioxidant activity compared to VMHAE (500 mg kg-1).
Deepen Pardeshi
Bombay college of Pharmacy, India
Title: Study of Microbial bio-transformation of volatile oils.
Time : 16:15-16:30
Biography:
Will be uploaded soon.
Abstract:
Microbial Biotransformation is the chemical reaction performed by micro-organisms and catalyzed by the enzymes within the microbial cell. When a substance cannot be synthesized artificially or is difficult to synthesize, then in such cases, biotransformation processes are useful. Products of biotransformation have a number of important pharmaceutical applications such as anticancer agent, antimicrobial agents, flavoring agents, etc. The current study comprises of evaluation of biotransformation of volatile oil components brought about by the yeast S. cerevisiae. We studied the effect of yeast on volatile oils namely clove oil, orange peel oil, herbs containing volatile oils namely Ajowan seed powder, and selective components of volatile oils namely vanillin and menthol. The orange peel oil was extracted by using hydrodistillation method. Sabouraud dextrose broth was used as culture media for the process. The volatile oil substrates viz. clove oil, orange peel oil, were introduced into the culture medium in concentration range of 0.1% to 0.5% v/v and ajowan powder, vanillin, and menthol were used in range of 0.1% to 0.5% w/v. The reactions were carried out for 10 days at incubation temperature of 37 °C. The reactions were monitored by HPTLC pattern of the chloroform extract of aliquots taken at the interval of 48 hours. The mobile phase used was Hexane-Ethyl Acetate-Glacial Acetic Acid (6.5:3.5:0.5). Amongst the five samples observed the vanillin and orange peel oil showed additional spots in TLC. Whereas, clove oil and menthol did not show any change in TLC pattern; indicative of inhibition of the cell growth at concentration of 0.1% and above. This is an important finding for the point of view of flavoring properties of products of biotransformation. Keywords: biotransformation, volatile oils, S. cerevisiae, Sabouraud dextrose broth, HPTLC.
Pankaj Bharali
Rajiv Gandhi University, India
Title: Biochemical constituents of four ethnobotanically important legume plant species from Arunachal Pradesh, India
Time : 16:30-16:45
Biography:
Pankaj Bharali is a PhD research scholar in Department of Botany, Rajiv Gandhi University, Arunachal Pradesh, under DST-INSPIRE scheme-New Delhi, Government of India.
Abstract:
Mankind is dependent on herbalism or botanical medicines since time immemorial. Well recognized as a biodiversity hot spot, Arunachal Pradesh, the lofty mountainous state of India, is blessed with rich variety of floral and faunal heritage greatly influenced by its diverse geography, topography and varied climate pattern. Among more than 500 medicinally significant plants, Albizia lucidior(Steud.)Nielsen, Acacia pennata (L.)Willd, Albizia chinensis (Osbeck) Merr. and Gymnocladus assamicus Kanj. ex P.C. Kanjilalare locally used as soap, shampoo, to treat stomach disorders and as lice repellent by the Adi people of the state. In view of the very important ethnobotanical properties, this study was carried out with the objectives of the detection and quantification of preliminary phytochemical constituents, quantitative study of the secondary metabolites, quantitative study of both micro- and macronutrients and evaluation of antioxidant activities of these four plant species. Preliminary phytochemical investigation indicated the presence of considerable amount of saponin in all the four species validating their use as a good source of soap or detergent. Among these four species studied, the seed pod of G. assamicus was found to containing highest amount of saponins. All of the four plant extracts showed significant antioxidant activity in DPPH antioxidant assay.
Ogbonna John Dike N
University of Nigeria, Nigeria
Title: Use of homo lipid for delivery of anti-malarial drugs
Biography:
Ogbonna is a Fellowship Scholar of The World Academy of Science (TWAS) at SNJB’s Shriman Sureshadada Jain College of Pharmacy, Neminagar, Chandwad, 423101, Nashik, India. He completed his Masters in Pharmaceutics, University of Nigeria, Nsukka. He has published more than 30 papers in reputable journals (local and international) and has been serving as an editorial board member of repute. His research interest is in natural products (plants especially polymers, lipids etc) as pharmaceutical excipients.
Abstract:
Solid lipid microparticles (SLMs) which are recent drug delivery system employed in the delivery of drugs in addition of having advantages of lipid formulations are biocompatible, less toxic, long term stability etc. In this study, lipid was extracted from the goat fat using wet rendering method. The lipid matrix was prepared by fusion method and the drug loaded (chloroquine phosphate, halofantrine, and artemether/lumefantrine) SLMs formulated by hot homogenization. The in vivo activity of the SLMs on Plasmodium berghei infected mice was done using Peter’s four day suppressive protocol and the histological studies performed after the mice were sacrificed. The SLMs were stable. The SLMs containing arthemeter/lumefantrine had 87.01 % parasite clearance while the commercial formulation of arthemeter/lumefantine had 80.0 % parasite clearance; SLMs containing chloroquine phosphate had 87.10 % parasite clearance while the commercial formulation of chloroquine phosphate had 84.12 % parasite clearance; SLMs containing halofantrine had 72.96 % parasite clearance while the commercial formulation of halofantrine had 85.71 % parasite clearance.The group G which received no treatment had 14.89 % parasite clearance. The SLMs formulation had good in vivo activity compared to the commercial formulatio and were not harmful to the vital organs of the mice therefore SLMs can be an alternative means of formulating these antimalarials.
Samkeliso Takaidza
Vaal University of Technology, South Africa
Title: Biological activities of species in the genus Tulbaghia: a review
Biography:
Samkeliso Takaidza is currently doing her first year of PhD in Biotechnology and works as a Research Technician at Vaal University of Technology where she completed MTech in Biotechnology in 2012. She graduated in 2005 with a BSc in Microbiology from University of Limpopo, South Africa. Her first degree was a BSc in Science with Education at Bindura University of Science and Technology, Zimbabwe.
Abstract:
Since time immemorial, plants have been used by several communities to treat a large number of diseases. Numerous studies on the pharmacology of medicinal plants have been done. Medicinal plants constitute a potential source for the production of new medicines and may complement conventional antimicrobials and probably decrease health costs. Phytochemical compounds in plants are known to be biologically active aiding, for example, as antioxidants and antimicrobials. The overwhelming challenge of drug resistance has resulted in an increasing trend towards using medicinal plants to treat various diseases, especially in developing countries. Species of the genus Tulbaghia has been widely used in traditional medicine to treat various ailments such rheumatism, fits, fever, earache, tuberculosis etc. It is believed that the species possess several therapeutic properties. This paper evaluates some of the biological activities of the genus Tulbaghia. It is evident from current literature that T. violacea is the most promising species. The other species of Tulbaghia still require further studies to ascertain their medicinal potential.
Syed Kasfur Rahman
MS Ramaiah Medical College, Bangalore, India
Title: Nature’s retort to the dengue crisis
Time : 17:00-17:15
Biography:
Will be uploaded soon.
Abstract:
Dengue, an arthropod borne viral disease, has recently been considered as a global threat due to its dramatic resurgence and involvement of vast geographical areas, infecting about 2.5 billion people every year.Owing to its four closely related, but antigenically distinct serotypes, an adequate drug or vaccine against dengue virus is not yet available. So far innumerable fruitless approaches have been tried for screening of compounds against this virus, such as structure-based computational discovery and targeting of the viral enzymes. Due to an increasing trend in complementary and alternative medicine in the last few decades, researchers have come up with natural compounds which can be considered safer as compared to the synthetic drugs available for this condition. Several medicinal plant extracts such as Psidium guava, Carica papaya, Euphorbia hirta etc. have been found to exhibit anti dengue activity through their wide spectrum of mechanisms of action such as, inhibition of mRNA of the virus, stimulation of genes that code for platelet production, membrane-stabilizing property and protection of blood cells against stress-induced destruction. This review highlights the manifold natural options available at present to alleviate the symptoms & hasten the recovery in these patients, when given as an adjunct to the standard regimens.
Biography:
will be uploaded soon
Abstract:
Dietary and phytochemical intervention are emerging as an important modality for cancer chemoprevention. Polymeric black tea polyphenols (PBPs) are the most abundant polyphenols in black tea, but the anti-initiating properties of PBPs, are poorly elucidated. In the present study, the mechanism of PBPs-mediated anti-initiation was investigated in mouse skin employing benzo[a]pyrene ( B[a]P) as a carcinogen. Methods: Animal studies were conducted after approval from the Institutional Animal Ethics Committee as per the Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA, Government of India) guidelines (Protocol No- 13/2010 , dated-March 28, 2012). On the dorsal skin of swiss bare mice topical pre-treatments with acetone (vehicle, 100 µl) or P3/P5/Pm (200µg in 100µl acetone) was carried out on three consecutive days. On the third day 20 minutes after topical application of acetone /P3/P5/Pm, animals were topically treated with B(a)P (1 mg, 100 µl acetone) and 24 hrs after the last application animals were sacrificed, skin was removed and stored at -80°C. Expression of Phase-I enzymes (CYP1A1 and CYP1A2), and their transcriptional regulators AhR (Aryl hydrocarbon receptor), AhR nuclear translocator (ARNT) , XAP-2 and HSP90 were studied by western blotting. Phase -1 Enzyme activity was studied by spectroflourometry and mRNA levels were checked with RT-PCR. Phosphorylation of AhR and association of AHR:ARNT was evaluated by immunoprecipitaton as well as binding of AHR to XRE was checked by EMSA and DNA adduct formation by immunohistochemistry. Simultaneously PGE2 levels and DNA damage were studies by ELISA. Results: Pretreatment of mice with PBPs significantly decreased B(a)P-induced enzyme activity, mRNA levels and protein levels of cytochrome P450 1A1 and 1A2. Although alone PBPs did not alter the basal levels of AhR, it significantly diminished the B(a)P-induced AhR levels, nuclear translocation, subsequent binding to DNA and ARNT. Pre-treatment of PBPs also significantly inhibit B(a)P-induced DNA adduct formation, level of PGE2 and 8-oh-dG in mouse skin. Conclusions: Black Tea polyphenols exhibit their anti-initiation activity via modulation of AhR, which is a transcriptional regulator of phase-I enzyme and subsequently by decreasing DNA adducts.
- Young Research Forum
Location: Hyderabad International Convention Centre, India
Session Introduction
S Gomathi
J K K Nattraja College of Pharmacy, India
Title: Phytochemical characterization and neuroprotective assessment of standardized extract of Pedalium murex Linn. leaves in endotoxemia-induced neurodegenerative model in rats
Time : 16:10-16:25
Biography:
Will be uploaded soon
Abstract:
An attempt has been made to understand the protective role of ethanol extract of Pedalium murex Linn. herbs (EEPM), by employing LPS to induce neurodegeneration. Animals were divided into six groups. The animals were exposed to LPS (xxmg/kg; i.p.) once (on day 1) and the changes in food and water intake, body weight and general behaviour were observed for 30 days and the effect of EEPM on the perturbations in antioxidant defence elements in hippocampal region of rat brain were also studied. Further, histopathological (hippocampal CA1) tests were carried out to substantiate the study. In addition, isolation, characterization and standardization of the chosen molecule were also carried out and the molecule was further subjected to in vitro antioxidant studies. Neurodegenerative diseases are one of the most debilitating, with respect to medical, financial and associated social issues. Currently, effective drugs for treating neurodegenerative diseases have led to discovery of new molecules from nature. Recent advances in the basic knowledge of such diseases have led to a re-evaluation involving new therapeutic approaches. Like other herbs, the neuroprotective effect of Pedalium murex in endotoxemia-induced neurodegenerative model is debatable and its neuropharmacological profile has not been investigated so far. This study may shed light on the potential role of Pedalium murex in such diseases and its development/discovery may herald a new therapeutic agent from nature.
Santosh Kumar Rath
CSIR-Indian Institute of Integrative Medicine, India
Title: Chemical investigation of bioactive secondary metabolites from Allamanda cathartica
Time : 16:25-16:40
Biography:
Santosh Kumar Rath is pursuing his Ph.D from CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu, (India) under the supervision of Dr. P. L. Sangwan, Scientist, BOC Division. He is registered for Ph.D in Chemical Sciences in AcSIR session Aug. 2013. He has one published paper in reputed journals. He is working on natural product isolation from plant origin and performs structural modification of major bioactive isolates.
Abstract:
Allamanda cathartica belongs to Apocynaceae family which is native to Brazil, also found in India and Srilanka that has an important medicinal value. In India it is mostly distributed in the eastern costal region of Odisha locally known as ‘Malatilata’, ‘Ghanta Phul’, ‘Harkakra’. The plant is traditionally used in Ayurvedic and Unani system of medicine for the treatment of various ailments such as healing of wounds and ulcers. The bark acts as a hydragogue in ascites while the leaves are a valuable cathartic in moderate doses. The root is used as a remedy for snake-bites. The whole plant is used against jaundice, malaria, fungal infections and antiseptic for the treatment of skin burns. Alcoholic and aqueous extracts of root possess hypotensive activity while leaf extract showed antifungal and anticancer properties. Considering its importance in Indian System of Medicine (ISM) and traditional use, the chemical investigation of whole plant was carried out which resulted in the isolation of eleven medicinally noteworthy secondary metabolites like allamandin (1), allamdin glucoside (2), betulinic acid (3), ursolic acid (4), 2,6,8-trihydroxy-3-(4-hydroxyphenyl)-4H-chromen-4-one (5), pinitol (6), heptacosanoic acid (7), heptacosane (8), β-sitosterol (9), β-sitosterol-3-O-glucoside (10), and sucrose (11). Betulinic acid, pinitol and flavonoid are reported first time from this plant. The structures of isolated compounds were elucidated mainly by one-dimensional, two-dimensional NMR, MS studies and by comparison with the spectral data in literature. Ursolic acid (4) was found major component (natural abundance: 1.118%), allamdin glucoside (2) (0.0353%) and betulinic acid (3) (0.019%) the least which is advantageous as all are known for potent anticancer, anti-microbial activities.
Tao Su
Hong Kong Baptist University, China
Title: Comparison of the toxicities of raw and processed Pinelliae rhizoma in rats
Time : 16:40-16:55
Biography:
Tao Su has completed her MPhil from Nanjing University of Chinese Medicine. Now, she is a PhD candidate of Hong Kong Baptist University. She has published more than ten papers in reputed journals. Her relevant research work is Chinese Pharmacology.
Abstract:
Pinelliae rhizoma (Banxia) is the dried tuber of Pinellia ternata. It has received great attention because of its excellent therapeutic effects as well as the associated toxicity. Banxia can cause irritant toxicity to oral, throat and gastrointestinal mucosa, as well as cardiotoxicity. To reduce the toxicity, Banxia is usually processed with ginger juice and alumen. The processed product is called Jiang-Banxia. Here, we used the blood biochemistry, histo-pathological examinations and metabonomics methods to compare the toxicities of Banxia and Jiang-Banxia in rats. Male SD rats were divided into three groups. Treatment groups received oral administration of Banxia or Jiang-Banxia powder suspended in 0.5% CMC-Na at a dose of 3 g/kg/day for 14 days, the control group received equal volume of the vehicle. Results showed that the Banxia-treated rats had smaller body weight gain and less physical activity compared to rats of control group, as well as obvious body trembling, aphony and diarrhea. Compared with the Banxia group, rats in Jiang-Banxia group showed better symptoms. The biochemical and histo-pathological results suggested that Banxia possessed toxicity, and Jiang-Banxia was less toxic. Differences of metabolic profiles involving serotonin and proline metabolisms were observed among vehicle-, Banxia- and Jiang-Banxia-treated rats, suggesting serotonin and proline metabolisms were involved in the toxicity of Banxia. Our data verified that processing with ginger juice and alumen can reduce the toxicity of Banxia, and shed new light on the pathological mechanism of Banxia.
Syed Kasfur Rahman
MS Ramaiah Medical College, India
Title: Nature’s retort to the dengue crisis
Time : 16:55-17:10
Biography:
Will be uploaded soon
Abstract:
Dengue, an arthropod borne viral disease, has recently been considered as a global threat due to its dramatic resurgence and involvement of vast geographical areas, infecting about 2.5 billion people every year.Owing to its four closely related, but antigenically distinct serotypes, an adequate drug or vaccine against dengue virus is not yet available. So far innumerable fruitless approaches have been tried for screening of compounds against this virus, such as structure-based computational discovery and targeting of the viral enzymes. Due to an increasing trend in complementary and alternative medicine in the last few decades, researchers have come up with natural compounds which can be considered safer as compared to the synthetic drugs available for this condition. Several medicinal plant extracts such as Psidium guava, Carica papaya, Euphorbia hirta etc. have been found to exhibit anti dengue activity through their wide spectrum of mechanisms of action such as, inhibition of mRNA of the virus, stimulation of genes that code for platelet production, membrane-stabilizing property and protection of blood cells against stress-induced destruction. This review highlights the manifold natural options available at present to alleviate the symptoms & hasten the recovery in these patients, when given as an adjunct to the standard regimens
Shubham Ukarande
Govt.college of Pharmacy,Aurangabad, India
Title: Phytochemical and Pharmacological studies of Trichodesma Indicum,(family:Boraginaceae)
Time : 17:10-17:25
Biography:
Shubham Ukarande is persuing B.pharmacy last year from Govt. College of pharmacy,A’bad.
Abstract:
Trichodesma indica (Boraginaceae) aerial parts is commonly used in the traditional systems of medicine against various ailments like urinary tract disorders, skin irritations, worm disturbances, as a component in health tonic preparation etc. The present study gives first insight of anti-microbial, anthelmintic, anti-oxidant properties and preliminary phytochemical analysis of the aerial parts of Trichodesma indica. All the analysis was done according to standard protocols.The ethanolic extract produced significant anti-bacterial, anti-fungal and anthelmintic properties in a dose-dependent manner. Preliminary phytochemical screening revealed the presence of alkaloids, steroids, flavonoids, tannins and phenols that may be the reason for its biological properties. This paper reporting the medicinal property of Trichodesma indica aerial parts and the further procedures of identification and isolation of active principles are in progress.
Rohit Gupta
Dadasaheb Balpande College of Pharmacy, India
Title: Effect of piperine on inhibition of FFA induced TLR4 mediated inflammation and amelioration of acetic acid induced ulcerative colitis in mice
Time : 17:25-17:40
Biography:
Rohit A. Gupta has completed his M. Pharm at the age of 24 years from Rashtrasanth Tukdoji Maharaj Nagpur University Nagpur from Institute of Pharmaceutical Education and Research, Wardha. He is working as Asst. professor at Dadasaheb Balpande College Of Pharmacy, Besa, Nagpur and had recently registered for his Phd at UDPS, Nagpur. He had published 2-books, 3-international papers and attended several national/international conferences. He is also serving as Director for Pharma Tutorials Pvt. Ltd, Nagpur a premier institute providing education for various entrance exams in Pharmaceutical science.
Abstract:
Ethnopharmacological relevance: Piperine, a main component of Piper longum Linn. and Piper nigrum Linn., is a plant alkaloid with a long history of medicinal use. Piperine exhibits antidepressant, hepatoprotective, anti-metastatic, anti thyroid, immunomodulatory, antitumor and anti-inflammatory activities; however its therapeutic potential in amelioration of ulcerative colitis and the underlying mechanism for anti-inflammatory activity remains unknown. Aim: The objective of the present investigation was to unravel the therapeutic potential of piperine on amelioration of IBD using acetic acid induced experimental animal model for ulcerative colitis and to determine the role of TLR4 receptor in signalling pathway of inflammatory gene expression in ulcerative colitis. Materials and Methods: We induced colitis using acetic acid (150µl of 5% once, intrarectally) in mice and estimated disease activity index (DAI), which took into account weight loss, stool consistency, occult/gross bleeding. Colon length, spleen weights, ulcer area and ulcer index were measured, Histological changes were observed by H&E staining. Effect of piperine on various antioxidant parameter of mice colon such as tissue myeloperoxidase (MPO) accumulation, SOD concentrations, reduced GSH and Lipid peroxidation were determined. Pro-inflammatory mediators, namely, nitric oxide (NO), tumor necrosis factor-α (TNF-α) were determined by TNF-α ELISA kit obtained from Thermo fisher scientific India pvt.ltd. Effect of piperine on haematological parameters of mice in acetic acid induced IBD was also determined which involves the estimation of FFA using commercial free fatty acid fluorometric assay kit. Result: Piperine significantly attenuated acetic acid induced DAI score which implies that it suppresses weight loss, diarrhoea, gross bleeding and infiltration of immune cells. Piperine administration also effectively and dose dependently prevented shortening of colon length and enlargement of spleen size. Histological examination indicated that piperine reduces edema in sub mucosa, cellular infiltration, reduced haemorrhages and ulceration as compare to acetic acid induced colitis in mice. Furthermore piperine inhibited abnormal secretion of pro-inflammatory mediators namely NO, cytokines TNF-α and reduces FFA induced TLR4 mediated inflammation. Conclusion: These results suggest that piperine has an anti-inflammatory effect at colorectal sites that is due to down- regulations of the productions and expression of inflammatory mediators and it also reduces FFA induced TLR4 mediated inflammation. Thus it may have therapeutic potential on amelioration of IBD. Keywords: Piperine, TNF-α, Ulcerative colitis, Free fatty acid (FFA), Toll like receptor (TLR4)
shyamala chennaboina
Kakatiya university, India
Title: Hepatoprotective activity of Murraya koenigii leaf extract on paracetamol induced hepatic damaged rats.
Time : 17:55-18:10
Biography:
Will be uploaded soon.
Abstract:
In the present study protective effect of Murraya koenigii leaf extract was investigated against paracetamol induced hepatotoxicity and compared with silymarin a standard hepatoprotective reference drug. Methanolic extract was prepared by uing soxhlet method. Male Albino wistar rats were divided into 5 groups. Rats except of control group received a single dose of paracetamol (2gm/kg.b.wt) on 7th day,silymarin (100mg/kg.b.wt) and extract (200 and 400mg/kg.b.wt ) for 7 days. On the 7th day blood samples were collected by retro orbital puncture and liver marker enzymes (sgot,sgpt,alkaline phosphatase),total protein,total bilirubin levels were estimated in serum and histopathologicl observations were done for the isolated livers. In the toxic group paracetamol treatment lead to elevated level of liver marker enzymes and bilirubin and there was deterioration of total protein content. However treatment with Murraya koenigii shows a significant results (p<0.05) in comparision with standard drug. Thus the results clearly demonstrate that murraya koenigii possess promising hepatoprotective activity,hence suggests its use as potential therapeutic agentfor protection from paracetamol overdose.
Biography:
Divya has completed her M.B.B.S from NTRUHS and currently pursuing M.D Pharmacology at S.S Institute Of Medical Sciences and Research Centre. She has undertaken a study on ‘ prescription pattern of drugs in Upper respiratory tract infections’
Abstract:
Ethnopharmacology is the scientific study correlating ethnic groups, their health, and how it relates physical habits and methodology in creating and using medicines. It can be defined as a multidisciplinary study of biologically active agents used in traditional medicine. As there are many disciplines that contribute to this branch, the research methodologies are diverse. Plants and plant based remedies constitute a complex mixture of compounds and identifying the active principles that are relevant for the pharmacological properties is not an easy task. Nevertheless the interest in identifying these compounds with therapeutic effects is on a rise, as they form the lead compounds for developing new drugs. The loss of language and traditional knowledge due to acculturation and plant habit destruction is a major concern especially among smaller tribes. Loss of indigenous knowledge has an impact on modern medicine. There is an imminent need of resurgence of ethnopharmacology as the knowledge of the use of plants is disappearing faster. Researchers in many disciplines including pharmacology, must accelerate the studies of ethnomedicine, for developing new drugs from natural products needed by humans.
Koushik Nandan Dutta
Assam Down Town University, India
Title: Effect of Mucuna pruriens (Linn) on Acute dermal toxicity, Electrocardiography & Respiratory variable of Wister rat
Biography:
Koushik Nandan Dutta has completed his M.Pharm in 2013 from Uttarakhand technical university,Dehradun and enrolled PhD in Assam Down Town University, Guwahati, Assam.He has completed 1 year project at Defence Research laboratory, Tezpur Assam. Currently he is working as a Assistant Professor in the department of pharmaceutical science in Assam Down Town University. He has published eight publications in reputed journals.
Abstract:
Mucuna pruriens(Linn) is Known as velvet bean or cowitch found in Africa, India and the Caribbean belonging to the family Fabaceae. Mucuna pruriens Linn is a herbaceous vine,generally tap rooted and reproducing by seeds,leaves have three leaflets up to 15 cm long,densely hairy beneath and rather silvery the lateral leaflets asymmetrical. Hair lining of mucuna seeds were tested for acute dermal toxicity. Hair lining were mixed with dusting powder and prepared three formulations according to concentration. This test was performed on rats based on OECD guideline number 404 (OECD, 1981a). In case of acute dermal toxicity study there was no treatment related mortality in any of the groups following topical application of formulations in either male and female rats for 14 days .Hematological Studies revealed that after application of tested formulation WBC like monocyte, neutrophil, eosinophil were increased. Serum biochemistry data did not produce significant differences between control and treated groups of both sexes in the majority of cases.In case of electrocardiography the animals showed normal value as compared to the control group. In whole body plethysmograph the treated animals showed similar value as compared to the normal group . After Sacrifice, the gross examination of the vital organs like liver, kidney and lungs, no noticeable hemorrhage and abnormal conditions were observed. The histopathological study did not showed any type of abnormality of skin in all treated group. It can be concluded from the finding of the present study that the findings of these studies support the safety use of the test substance Mucuna pruriens.
Biography:
Purbajit Chetia has completed his M.Pharm in 2012 from Sikkim University and enrolled PhD in Assam Down Town University, Guwahati, Assam. Currently he is working as a Assistant Professor in the department of pharmaceutical science in Assam Down Town University. He is having a few research publication in reputed journals. His area of interest in Rheumatoid Arthritis.
Abstract:
Rheumatoid arthritis (RA) is a chronic, autoimmune and systemic inflammatory disorder that mainly attacks synovial joints resulting in an unchecked synovial inflammation that leads to increased oxidative stress in peripheral blood inflammatory cells. RA typically affects the joints of hands and feet, although any joint in the body may be affected such as wrists, knees, hips and shoulders resulting in permanent damage, bone destruction and deformity. The precise etiology of RA remains still unknown, but studies have implicated a role for oxidative stress and redox signaling in its pathogenesis. Lymphocytes are considered to play a key role in the pathogenesis of inflammatory diseases like RA. The disease is consistently associated with an increase in various pro-inflammatory factors that include cytokines (IL-1 β, IL-6, tumor necrosis factor alpha TNF- α), prostaglandins and also reactive oxygen and nitrogen species (ROS/RNS) at sites of inflammation that leads to damaging of cellular elements in cartilage and components of the extracellular matrix like protein and lipid. This present study was aimed to evaluate the ex vivo effect of plant derived biomolecules (β-carotene and Naringenin, which are already proved to have free radical scavenging property) on generation of free radicals in isolated blood lymphocytes of patients with RA. Interestingly it was found that these biomolecules significantly inhibited the superoxide, hydroxyl and NO radicals attenuating oxidative stress in the isolated peripheral blood mononuclear lymphocytes of patients with RA that admire further studies on other human inflammatory cells.
Prasanthi doppalapudi
Sri Padmavathi Mahila Visvavidyalayam, India
Title: Pharmacognostic and physicochemical analysis of the seeds of citrullus lanatus l
Time : 18:10-18:25
Biography:
Prasanthi doppalapudi completed her B-Pharmacy and M-Pharmacy from Dr. H. L.T. College of Pharmacy, Kengal, Channapatna, Bangalore rural district, Karnataka. I am pursuing my Ph.D. as a fulltime research scholar under the guidance of Prof. A. Sreedevi in the Institute of Pharmaceutical Technology, Sri Padmavati Mahila Visvavidyalayam, Tirupati, Andhra Pradesh. I am a Principal Investigator of DST-WOS-A Project. Three articles were published in reputed journals and attended 2 international and 14 national conferences.
Abstract:
The main objective of the present study was to evaluate pharmacognostic properties including macroscopic, microscopic and physicochemical characters of the seeds of Citrullus lanatus. Citrullus lanatus seeds used for this study were purchased from Srisu Agrichem Private Ltd., Kalyanpuri, Uppal and authenticated by Botanist Dr. Madhava chetty, Herbarium keeper, Department of botany, Sri Venkateswara University, Tirupati, India and a specimen (Specimen No:640) has been deposited in Department of Botany, Sri Venkateswara University, Tirupati, India. Seeds were allowed for shade drying (except for microscopic studies) and seeds were subjected for pharmacognostic study including microscopic and macroscopic characters of the seed and seed powder, physico-chemical parameters such as total ash value, acid insoluble ash, water soluble ash, alcohol soluble extractive, water soluble extractive value, pH, moisture content, foreign matter present, foaming index, swelling index, etc. Above mentioned parameters were carried out by following standard methods. Fluorescence analysis of the seeds was conducted. Preliminary phytochemical studies were also conducted. Microscopic studies revealed the presence of thick walled trichomatous epidermis with mucilaginous hairs, sclerenchymatous hypodermis and wide circular parenchymatous inner seed coat. Physicochemical parameters had shown characteristic results such as total ash value of 3.5%, and pH ranging from 6.5-6.55 for 1% solution and 5.90 to 5.95 for 10% solution. Preliminary phytochemical studies revealed the presence of carbohydrates, tannins, glycosides, steroids, saponins, flavonoids and oils and fats. The present study i.e., pharmacognostic studies of Citrullus lanatus is helpful in the characterization of the crude drug.
- Track 2: Analytical Techniques in Phytochemistry
Track 3&11: Herbal Drugs & Formulations, Herbal System of Medicines
Track 4&9: Drugs from Natural Sources, Natural Products of Medicinal Interest
Track 10&12: Crude Drugs & Plant Products, Plant Biotechnology & Tissue Culture
Location: Hyderabad International Convention Centre, India
Session Introduction
Wilfred T Mabusela
University of the Western Cape, South Africa
Title: Phytochemical studies of selected south african medicinal plants
Time : 12:00-12:20
Biography:
Wilfred Mabusela completed his PhD at the University of Cape Town, South Africa, and this was followed by two years of postdoctoral studies at the same institution, which extended his doctoral work on structural studies of plant polysaccharides. He subsequently took up a lecturing position at the University of the Western Cape where he is currently an associate professor. He has published more than 30 papers in reputable journals, and has been a visiting scientist at institutions in Europe and North America.
Abstract:
Traditional medicine is a cultural practice with a long history in South Africa and involves the use of approximately 3000 plants, out of a national biodiversity represented by about 30 000 higher plant species. A number of these medicinal plants have been investigated in our lab for their phytochemical composition, and this paper will describe some of the contributions which we have made in this regard during the last few years. Selected plant species, which included Sutherlandia (Lessertia) frutescens, Galenia africana, Cissampelos capensis, and Gethyllis multifolia among others, were subjected to extraction using a variety of organic solvents followed by chromatographic fractionation of the extracts obtained. Isolated compounds were examined for their chemical structural features with the aid of nuclear magnetic resonance and mass spectroscopy, leading to the identification of compounds belonging to the following classes: alkaloids, flavonoids and, triterpenoid- and flavonoid- glycosides, some of which were of novel character. Some of the crude extracts and purified compounds were also studied for cytotoxicity using the brine shrimp lethality bioassay, and for antimicrobial activity against gram-negative and gram positive bacteria as well as fungal species.
Sneha J Anarthe
Osmania University, India
Title: Sphaeranthus indicus- As natural immunomodulator
Time : 12:20-12:40
Biography:
Sneha has completed her PhD from JNTU, hyderabad in 2012 and having 10 yrs of teaching experience. She is working as Associate Professor in Gokaraju Rangaraju College of Pharmacy, Hyderabad. She has pulished more than 25 research article in reputed journals
Abstract:
The present investigation is to evaluate the immunomodulatory activity for methanolic extract of Sphaeranthus indicus Linn. at different doses as 100, 200 and 400 mg/kg bd.wt. in healthy wistar albino rats. The assessment of immunomodulatory activity was evaluated for humoral immunity (antibody titre, plaque forming cell assay and quantitative haemolysis of SRBC) and cellular immunity (delayed type hypersensitivity, T- cell population and drug induced myelosuppression) with antigen challenge by sheep RBCs. Methanolic extract of Sphaeranthus indicus along with the antigen (sheep red blood cells) showed significant increase in the circulating antibody titer and the number of plaque forming cells (PFC) in the spleen at the dose of 100 mg/kg bd.wt. as compare to 200 and 400 mg/kg bd.wt. Methanolic extract of Sphaeranthus indicus also showed significant (p < 0.01) increase in the DTH response, restoration of histological parameters, increase in lymphocytes and rosettes formation in T-cell population at dose of 400 mg/kg bd.wt. as compare to 100 and 200 mg/kg bd.wt. The present investigation reveals that Sphaeranthus indicus can be used as immunomodulator for activation of specific and non- specific immune responses.
Hassan Abdalla Almahy Dafalla
Taif University, Kingdom of Saudi Arabia
Title: Chemical constituents of the leaves of Ficus nitida and their antimicrobial activity
Time : 12:40-13:00
Biography:
Hassan Abdalla Almahy Dafalla has completed his PhD from University Putra Malaysia. He is the director of a premier Bio-Soft service organization. He has published more than 28 papers in reputed journals
Abstract:
The dichloromethane extract of the air-dried leaves of Ficus nitida belongs to the family Moraceae afforded lutein (1), β-sitosterol (2), stigmasterol (3), chlorophyll a (4), phytol (5), (E)-3-alkenoic acid (6), triglycerides, fatty alcohols, and fatty acids, while the twigs yielded saturated fatty acid ester. The structures of 1-5 on the basis of spectroscopic data such as IR, UV, 1H-NMR, 13C-NMR, COSY, HMQC, HMBC, DEPT and MS and confirmed by comparison data with those reported in the literature. The structure of 6 was elucidated by 1D and 2D NMR spectroscopy. However, bioactivity screening showed that the pure isolated compounds possessed activity on two species of bacteria Bacillus cereus NRRLUI-1447 and Pseudomonas aeruginosa UI-60690 and no activity against four species of fungi (Aspergillus ochraceus NRRL 398, Candida lipolytica ATCC 2075, Sacchromyces cereviseae NRRL 2034 and Sacchromyces lipolytica).
Sathyanarayana B
Muniyal Institute of Ayurveda Medical Sciences, India
Title: Ayurvedic bioenhancers: A classical and contemporary review
Time : 13:30-13:50
Biography:
Sathyanarayana B is an MD in Ayurveda in the subject Bhaishajya Kalpana (Ayurvedic pharmaceutics) from IPGT and RA, Jamnagar. He is currently working as Principal in Muniyal Institute of Ayurveda Medical Sciences, Manipal. He is also working as Research Executive and MR for ISO in Muniyal Ayurveda Research Centre. He has teaching, research, pharmaceutical and clinical experience of 14 years and administrative experience of five years. He has presented more than 40 papers as resource person in various national and international conferences, besides chairing the scientific sessions. He has over 20 publications across various national and international journals besides being a Reviewer for three indexed international journals.
Abstract:
Herbal bioenhancer is an agent of herbal origin or any phytomolecule, which is capable of enhancing bioavailability and bio-efficacy of a particular drug or nutrient with which it is combined, without any typical pharmacological activity of its own at the dose used. Maximizing bioavailability is therapeutically important because the extent of bioavailability directly influences plasma concentrations and consequently therapeutic efficacy. Bioavailability enhancement can make the expensive drugs affordable and reduce the toxic effects by reducing the required dose of drugs. A bioenhancer may act by different mechanisms like, promoting the absorption of the drugs from gastrointestinal tract, inhibiting or reducing the rate of biotransformation of drugs in the liver or intestines, modifying the immune system in such a way that the overall requirement of the drug is reduced substantially, etc. Ayurveda the science of life is based on highly scientific principles. The one of the oldest system of medicine, Ayurveda is momentous in audience of worldwide on virtue of its holistic approach of life. Ayurvedic pharmaceutics is highly advanced explaining over ten thousand formulations under different categories like Kashaya, Vati, Asava-arishta, Avaleha, Paka, Khanda, Bhasma, Pishti, etc. Formulations of Ayurveda consist of substances of herbal, mineral/metal and animal origin which are processed pharmaceutical to have therapeutic effects. Ayurveda explain different processes to enhance the bioavailability of the drugs, e.g., Bhavana, Marana (to produce incinerated products containing nano particles), Snehapaka (to form oleaginous products akin to liposomes), Ksheerapaka, etc. It also explains the concepts like Yogavahi, drugs like Pippali (Piper longum), Shilajatu, Kajjali, Honey, Ghee, etc., that are scientifically established as bioenhancers. Current presentation throws a light on various processes and drugs in Ayurveda improving bioavailability. Their role in the design and development of formulation also will be discussed.
Ranjitsinh Devkar
The M S University of Baroda, India
Title: Anthocyanin rich extract from red cabbage (Brassica oleracea) reduces oxidative stress and prevents apoptosis in stressed rat cardiomyocytes
Time : 13:50-14:10
Biography:
Ranjitsinh Devkar is currently an Assistant Professor at the M S University of Baroda, India. His primary focus is on atherosclerosis and related lifestyle disorders. His work focuses on bioactive compounds of ethnomedicinal origin and their mechanism in alleviating the said diseases in relevant experimental models. He has about 70 publication and conference presentation history and is expanding research into three main areas viz. anthocyanins to alleviate atherosclerotic inflammation and myocardial necrosis, use of functional foods in mitigating atherosclerosis, and finding novel therapeutants in an endemic ethnomedicinal Indian herb.
Abstract:
The colourful bioflavonoids are highly publicized for their free-radical scavenging and antioxidant capacities and form the basis of preventive medicine. Anthocyanins have been reported to be protective against a myriad of human diseases including myocardial infarction, atherosclerosis and cancer. This study assesses therapeutic potential of anthocyanin rich extract of Brassica oleracea (Red cabbage; ARCE) against experimentally induced oxidative damage in H9C2 (rat cardiomyocytes) cells through a carefully scripted protocol. ARCE was prepared and validated using standard procedures and H9C2 cells were pre-treated with the same (250 µg/ml) for 24 h. Later, cells were subjected to H2O2 (100 µM) induced oxidative stress (12 h). Cytotoxicity assay showed significantly less (5.86%) mortality in ARCE+H2O2 treated group as compared as compared to the H2O2 group (48.44%). Also, H2O2 treatment accounted for intracellular high oxidative stress and lower mitochondrial membrane potential. The same was comparable to control values in ARCE+H2O2 treated groups. Also, the expression of antioxidant (SOD and CAT), apoptosis (BAX and Bcl2) and cardiac marker (Caveolin 3) genes were assessed that further corroborated protective role of ARCE against H2O2 induced cardiotoxicity. ARCE pre-treatment also accounted for decreased indices of apoptosis in ARCE+H2O2 (19.1%) group as compared to H2O2 treated group (57.3%). These observations are first report on cardioprotective potential of anthocyanins from ARCE that add further value to Brassica oleracea as a functional food.
Dipal Gandhi
Nirma University, India
Title: Anticancer potential of Dillenia indica and Dillenia pentagyna plants and its correlation with presence of active phytoconstituent
Time : 14:10-14:30
Biography:
Dipal Gandhi has completed her BPharm and MPharm from L M College of Pharmacy, and PhD from Nirma University, Ahmedabad Gujarat. She is working as an Assistant Professor, Department of Pharmacognosy at Institute of Pharmacy, Nirma University since last 8 years. Her area of research work is mainly in phytochemical investigation, standardization and quality control of herbal drugs, analytical method development of phytoconstituents and evaluation of their pharmacological activity.
Abstract:
The present investigation was aimed to develop simple, sensitive, fast, and accurate RP-HPLC method for estimation of betulinic acid from bark and leaf of D. indica and D. pentagyna, widely distributed in forest regions of India, is used as a traditional medicine for the treatment of cancer and cardio vascular diseases. HPLC separation was achieved on a C18 Purosphere Star Hyber (250x4.6 mm, 5 μm, Merck). The mobile phase consisting of acetonitrile and water (93:7 v/v) with flow rate 1.2 ml/min at 207 nm. For estimation of betulinic acid, different fractions such as benzene and ethyl acetate using bark and leaves of D. indica were prepared. Chromatogram showed peak of betulinic acid at Rt 6.308 min. Analysis of data showed that the method is reproducible and selective for the estimation of betulinic acid. Based on results obtained by RP-HPLC method, prepared fractions were evaluated for its anticancer potential. MTT assay has been performed on three different cell lines HCT-15, DU145 and A-375 which explains role of betulinic acid and the anticancer potential of plants.
Preety Panwar
University of British Columbia, Canada
Title: Anti-osteoporotic cathepsin K inhibitors from Salvia miltiorrhiza
Time : 14:30-14:50
Biography:
Preety Panwar completed her PhD and is a Postdoctoral Fellow in Dieter Bromme’s group at University of British Columbia. She has completed her PhD entitled “Targeted and sustained drug delivery using liposomal formulations” from G B P U A T University, India in February 2011. Currently, she is investigating the mechanism of cathepsin K mediated extracellular matrix degradation, and identification of specific anti-collagenase inhibitors of cathepsin K and their in vitro and in vivo evaluation. She has published many papers in reputed journals.
Abstract:
Inhibition of osteoclastic Cathepsin K (CatK) is of major interest for the development of antiresorptive drugs and the treatment of osteoporosis. In this study, we propose ectosteric inhibitors that specifically block the collagenase activity of CatK without interfering with its catalytic site. We discovered three compounds (T01, T04, and T06) which differentially inhibit the in vitro CatK-mediated degradation of soluble and fiber collagen. Here, we demonstrate that T06 is the most potent ectosteric inhibitor of CatK for both soluble and fiber collagen degradation whereas T01 and T04 are comparatively weaker inhibitors but appear to discriminate between the inhibition of soluble and fibrous collagens. The bone antiresorptive efficacy of these compounds was analyzed in human osteoclasts assays and compared with the highly potent active site-directed inhibitor, odanacatib. None of the compounds affected the metabolic activity and viability of osteoclasts. T01 and T06 as well as odanacatib strongly inhibited the overall bone resorption, the generation of CatK-released collagen fragments (CTx-assay), and abolished the formation of trenches by about 55% for the resorption surface, 50% for resorption depth, and 63% for CTx release. On the other hand, T04 exhibited no effect on the formation of trenches and release of CTx, which may indicate a metabolic inactivation of the compound in the osteoclast assay. Our observations show that ectosteric inhibitors extracted from Salvia miltiorrhiza have a similar potency as active site-directed inhibitors of the protease and may constitute a novel approach for anti-resorptive therapy.
Sandip Mali
Pitambari Products Pvt.ltd, India
Title: Preparation of stable & potentized gomutra formulation “Gomutra plus capsule†w.r.t conventional forms of gomutra
Time : 14:50-15:10
Biography:
Will be uploaded soon
Abstract:
The science of natural alternative medicine, Ayurveda (meaning: the ‘knowledge of life’) originated in India. Use of naturally-occurring materials for therapeutic as well as prophylactic purposes is characteristic to the practice of Ayurveda. Gomutra / Cow urine has been elaborately explained in Ayurveda and described in "Sushruta Samhita", "Ashtanga Sangraha" and other Ayurvedic texts as an effective medicinal substance/secretion of animal origin with innumerable therapeutic properties. Bhav- Prakash Nighantu describes gomutra as the best of all types of animal urine (including human) and enumerates its various therapeutic uses. Gomutra is called "Sanjivani" and "Amrita" in Ayurveda. In spite of having so many medicinal properties Gomutra is not used efficiently because of limited access and availability of cow’s urine for regular mass consumption, unpleasant the taste and odor ,stability of gomutra, loss of bioactive component during processing. To Overcome these limitation Gomutra is converted to powder form under extremely low pressure & high temperature of short duration which allows to restore bioactive as present in Gomutra. Comparitive physic-chemical analysis also reveals Bioactive wise superiority of Gomutra powder w.r.t Ark & Ghanavati. By further enrichment with herbs Amala(Embelica officinalis), Harada(Terminalia chebula), Behada (Terminalia balerica); Gomutra Plus capsules has advantages of Synergistic combination of Gomutra powder and Triphala extract; Stabilized, odor-free, concentrated, and potentized formulation of Gomutra; Higher efficacy in small pharmaceutical doses.Though it improves the stability but also preserve bioactive composition of Gomutra at greater extent, unlike to Ark & Ghanavati.
Pramod Hurkadale
K L E University, India
Title: In vitro studies on Achyranthes coynei Santapau. for micropropagation
Time : 15:10-15:30
Biography:
Pramod H J is the Professor and Head, Department of Pharmaceutical Biotechnology, K L E University’s College of Pharmacy, Belagavi, India and has completed his BPharm degree from Kuvempu University, Shivamogga obtained his MPharm degree with distinction from Rajiv Gandhi University of Health Sciences, Bangalore. He was awarded PhD in Pharmacy from Rajiv Gandhi University of Health Sciences, Bengaluru under AICTE sponsored quality improvement program. He has also been invited as Speaker for international conferences at South Africa, Malaysia and Egypt. He is has been awarded Batch of the Best Award from Zydus-Indon Cadila Health Care Ltd, Ahmedabad. His areas of research interest are herbal formulations/excipients, bio-surfactants, traditional medicines and its screening for various pharmacological activities and jointly collaborated with Regional Medical Research Centre, ICMR, Belagavi on tissue culture studies of Medicinal Plants from Western Ghats region.
Abstract:
Achyranthes coynei Santapau., a rare, endemic medicinal plant was successfully propagated in vitro using apical shoot tips as explants. A combination of 3.0 mg.L-1 of 6- Benzylaminopurine (BAP), 0.5 mg.L-1 Thidiazuron (TDZ) and 0.5 mg.L-1 Kinetin (KN) in Murashige and Skoog (MS) agar-solidified medium proved the best combination for multiple shoot induction, yielding 408 shoots per explants after 60 days of culture. The shoots were rooted with a frequency of 80% after 30 days of culture on MS agar medium fortified with IBA/NAA (0.5 mg.L-1) before being acclimatized in the green house.
Radhakrishna G Pillai
University of Calicut, India
Title: Curcumin prolongs graft survival in mouse corneal allografts.
Time : 15:30-15:50
Biography:
Radhakrishna G Pillai has completed his PhD from the Department of Biochemistry, University of Kerala and postdoctoral studies from School of Medicine, University of California, San Dieg, USA (200-2001) and Imperial College London (2001-2007). Now he is working as Assistant Professor at the Department of Life Sciences, University of Kerala. He has published more than 30 papers in reputed journals and has been serving as an editorial board member of various reputed journals.
Abstract:
Curcumin is a dietary pigment from the plant Curcuma longa with known antioxidant, antineoplastic and anti-inflammatory effects. It is quite safe and has been ingested by people for centuries without significant reports of intoxication. Acute and subchronic toxicology studies showed limited adverse effects. The immunosuppressive and graft protective effects of curcumin were studied in mouse corneal transplant models. Curcumin significantly increased the mean survival time (MST) to 32 days, as compared to 14 days among non treated controls. After transplantation there was significant increase in the level of chemokines and cytokines in corneal tissue, which was reduced by an ip treatment with 50mg/kg curcumin for 7 days. On treatment with curcumin a decrease in the expression level of inducible Nitric Oxide Synthase (iNOS) mRNA and production of nitric oxide by the corneal tissue were also observed. This study for the first time demonstrates the effectiveness of curcumin as a novel immunosuppressant after corneal transplantation. This also reveals the protective effect of curcumin on graft survival.
Giuliana Vila Verde
Universidade Estadual de Goiás, Brazil
Title: Optimization of the extraction process for quality control of essential oils obtained from the fruit of Pterodon emarginatus (Fabaceae) occurrent on Brazilian Savana
Time : 15:50-16:10
Biography:
Giuliana has completed PhD in 2011 and her postdoctoral studies in Molecular Biology from the Federal University of Goiás in 2014, directed by Prof. Dr. José Realino de Paula. She is currently Professor of Pharmacognosy holder at the State University of Goiás and collegiate member of two Sensu strict Masters Courses as Molecular Sciences and Territories and Cerrado Cultural Expressions. Her researches have emphasis on Ethnobotany, phytochemistry, quality control, extraction of compounds by irradiation of microwave and biological assays of Savanas´medicinal plants specially Erythroxylaceae and Fabaceae. Member of the Multidisciplinary Network for Sustainable Production Animal Pro-Midwest, Brazil, foccusing on optimization and extraction of organic compounds for the development of bioproducts.
Abstract:
The Pterodon emarginatus specie (Fabaceae) is popularly known as "white sucupira" or "faveiro" that grows on Brazilian Savana. Under Brazilian folk medicine its fruits are used mainly for their antinociceptive properties, anti-inflammatory, antimicrobial and antioxidant. Fruit´s oil and extracts of bark and stems have antimicrobial, anti-inflammatory, analgesic, antirreumática and help control diabetes. Thus, it is used on the treatment of muscle aches, sprains, arthritis and arthrosis. In the Midwest region, the population take stem bark´s tea for gynecological infections.The reference method for the extraction of essential oil is by Clevenger apparatus, and the hydrodistillation enhanced by microwave method to be tested and potentially replaceable. The goal was to optimize the essential oil extraction technique of the fruits of sucupira by irradiation of microwave and characterize the volatile compounds with a view to quality control and species identification. The fruits of sucupira were subjected to oil extraction second literature´s protocols. The process conditions were established in Delineation Central Composite Rotational (CCRD) and the results were evaluated by applying Response Surface Methodology (RSM). Waste analysis was performed at 95% confidence limit (p <0.05), which consisted of the significance test of model fit, based on the analysis of variance (ANOVA). Reaction time and moisture conditions were standardized for these analyses. Under these conditions, the yield is 6.6% which sets the optimum adaptation of procedures to the model. So our objective of optimizing the extraction method was hit. The results are important to contribute to stablishment of quality control parameters of this drug and also, getting less time, saving energy and solvents, which puts us in line with green chemistry
Umesh K Patil
Dr H S Gour Central University, India
Title: Metabolomics and ethno-pharmacology: A multidisciplinary approach in novel drug discovery from natural sources
Biography:
U K Patil has completed his PhD from Dr H S Gour University, Sagar and Postdoctoral studies from Institute of Biology, Leiden University. Presently, he is serving as General Secretary to Indian Society of Pharmacognosy. His area of specialization is Herbal Drug Technology and Natural Products. He has more than 15 years of experience in teaching and research. He is recipient of 7 prestigious awards given in the field of HMPs and remained BOYSCAST Fellow of DST. He is a Member of many professional bodies including International Pharmaceutical Federation (FIP), International Society of Ethno-pharmacology (ISE), International Society for Medicinal Plant Research (GA), Germany, International Pharmaceutical Students Federation, International Society of Natural Product Development, APTI, IPA, IPGA, ISTE and IAHP. He has published more than 100 papers in reputed journals and has been serving as Editorial Board Member of many reputed journals.
Abstract:
Phytochemical screening techniques are developing fast and we now have tools available which allow us to analyze complex mixtures in ultra modern ways. The modern research era allows a systematic investigation of such complex mixtures and specifically to link phytochemical analysis with other strategies (such as in vitro or in vivo screening for biological activity or toxicity, morphological plant diversity and ecological parameters). Specifically, as it relates to the study of medicinal and food plants, the main challenge is to understand the complex effects of such extracts. Since most of these research activities are linked to plants used in traditional and local cultures, this requires an ethno-pharmacological approach coupled with modern metabolomic investigations. The potential of such multidisciplinary approach and its potential benefits for phytochemically oriented research will be discussed. The traditional medicines offer a rich and largely unexplored source of therapeutic leads for the pharmaceutical industry. Academic research and the pharmaceutical sciences may profit in many ways by including ethno-pharmacological and metabolomic investigations. One highly illuminating example is the acetylcholine-esterase inhibitor galanthamine. The early research into the botanical species was derived from Galanthus species. It was based on local, but poorly documented knowledge, which was then turned into a medication to treat poliomyelitis and ultimately resulted in a novel treatment of Alzheimer’s disease. Although Herbal Medicinal Products (HMPs) have been perceived by the public as relatively low risk, there has been more recognition of the potential risks associated with this type of product as the use of HMPs increases. Potential harm can occur via inherent toxicity of herbs, as well as from contamination, adulteration, plant misidentification, and interactions with other herbal products or pharmaceutical drugs. Regulatory safety assessment for HMPs relies on both the assessment of cases of adverse reactions and the review of published toxicity information. However, the conduct of such an integrated investigation has many challenges in terms of the quantity and quality of information. Adverse reactions are under-reported, product quality may be less than ideal, herbs have a complex composition and there is lack of information on the toxicity of medicinal herbs or their constituents. Nevertheless, opportunities exist to capitalize on newer information to increase the current body of scientific evidence. The integration of ethno-pharmacology and metabolomics can reduce the uncertainty in decision making with respect to herbal medicinal products.
Rajiv Dahiya
President, Association of Pharmacy Professionals (APP), India
Title: Complex cyclic oligopeptides: Synthesis and biological properties
Time : 16:25-16:45
Biography:
Rajiv Dahiya is Doctor of Science in Clinical Pharmacology from International University for Complementary Medicine, Colombo, Sri Lanka and also holds PhD in Pharmacy from Uttar Pradesh Technical University, Lucknow, Uttar Pradesh, India. He is presently President of Association of Pharmacy Professionals (APP), Editor-in-Chief of international level journal - Bulletin of Pharmaceutical Research (BPR) and Principal at Globus College of Pharmacy, Bhopal, Madhya Pradesh, India. He has 13 years of teaching and 10 years of research experience. His research area is synthetic peptide chemistry and until now, he has published 51 research papers and 9 review articles in various international and national journals covering a total impact factor of 36.6. He is the recipient of ‘Innovative Researcher Award’ in Jun 2012; ‘Excellence Award’ in Pharmacy in Feb 2014; ‘Young Pharmacist Award’ & ‘Young Scientist Award’ in Mar 2014; ‘Distinguished Pharmacy Professional Award’ in Oct 2014 and ‘Young Investigator Award’ in Mar 2015.
Abstract:
Natural products are the active components not only of most traditional medicines but also many modern medicines. They have pharmacological or biological activity that can be of therapeutic benefit in treating disorders. Some current medicines are obtained directly from natural sources but other medicines are developed from the natural product lead originally obtained from the natural source. Among wide range of natural products, complex cyclic oligopeptides peptides are of special interest for their enhanced stability and pharmacologic properties. Due to their limited conformational flexibility, cyclic peptides with C-to-N-terminal peptide bond and a disulfide bridge can confer high target binding affinity and resistance to proteolytic enzymes. Peptides and proteins are attractive initial leads for the rational design of bioactive molecules. Several natural cyclic peptides have recently emerged as templates for drug design due to their resistance to chemical or enzymatic hydrolysis and high selectivity to receptors. Further, bicyclic peptides can bind with high affinity and selectivity to protein targets, making this format attractive for biotechnological and medicinal applications. The good binding properties are based to a large extent on the limited conformational flexibility of the two connected peptide rings. On the other hand, Host Defense Peptides (HDPs), small cationic peptides, play a vital role in innate immunity response and immuno-modulatory stimulation. Antimicrobial Peptides (AMPs) like β-defensins and cathelicidin, are involved in the defence against pathogenic organisms, e.g., the antimicrobial activity of saliva largely depends on histidine-rich AMPs (histatins). These complex cyclic congeners being isolated from higher plants and marine resources, possess modified amino acid residues like DHHA, ADHA, AHOA, AHMP and exhibit their pharmacological properties through binding to corresponding enzymes which allow cyclic oligopeptides to act as therapeutic agents in resistant world.
Suresh V Chennupati
Mother Teresa Pharmacy College, India
Title: Traditional medicine (Ayurveda): Inspired approaches to drug discovery
Time : 16:45-17:05
Biography:
Suresh V Chennupati has completed his PhD from Acharya Nagarjuna University. He is the Principal of Mother Teresa Pharmacy College, one of the premier colleges in Khammam District. He has published more than 25 papers in reputed journals and has been serving as an Editorial Board Member of repute. He received Bharat Siksha Ratan, International Award for Educational Excellence, APP Young Performer Awards. He is the Co-Author of Pharmaceutical Inorganic Chemistry Text Book Published by Pharma MedPress. He is Executive Editor for an online journal titled Journal of Applicable Chemistry and is also the Andhra Pradesh State Branch President for Association of Pharmacy Professionals.
Abstract:
Ayurveda is an Indian traditional system of medicine. It was originated in ancient India many thousand years ago. The origin, development and practice of Ayurveda have many dimensions and complex theories based on religion, faith and ancient Vedic science. Ayurveda is one of the official systems of medicine in India and it is also widely practiced in many other countries. Considerable research on Pharmacognosy, Photochemistry, Pharmacology and Clinical therapeutics has been carried out on Ayurvedic medicinal plants. Many pharmaceutical companies changed their strategies towards natural product drug discovery and it is important to follow systems biological applications to facilitate the process. Number of drugs are entered the International Pharmacopoeia through the study of ethno pharmacology and traditional medicine. For Ayurveda and other traditional medicines, newer guidelines of standardization, manufacture and quality control are required. Drug discovery strategies based on natural products and traditional medicines are the attractive options. Drug discovery and development need not always be restricted to new molecular entities. Traditional knowledge give an idea for drug development can fallow reverse pharmacology approach and reduce the time and cost of process are expected to takes place mainly from innovation in drug target elucidation and lead structure discovery. Powerful new technologies such as automated separation techniques, high- throughput screening and combinational chemistry are revolutionizing drug discovery. Traditional knowledge can offer smart strategy for new drug candidates to facilitate discovery process and also for the development of rational synergistic botanical formulations.
Muralidhar Rao Akkaladevi
Maheshwara Institute of Pharmacy, India
Title: Nano formulations of bioactive natural products for the treatment of cancer
Time : 17:05-17:25
Biography:
Muralidhar Rao is eminent teacher in Pharmaceutical Sciences. Currently, he is serving as Professor & Principal at Maheshwara Institute of Pharmacy, Patancheru, Hyderabad. He did his B.Pharm, M.Pharm & PhD (Pharmaceutics) from Kakatiya University, Warangal. He received Senior Research Fellowship (SRF) from AICTE, New Delhi; Best Presentation (Oral) Award in National convention of Association of Pharmacy Professionals, Bhopal, India ; “APP Principal of the year Award” at DD university Gujarat; “Bharat Excellence Award at IIC, New Delhi. He is President, Association of Pharmacy professionals, Telangana State branch. He is the fellow member of Association of Pharmacy Professionals (FAPP), International Science congress Association (FISCA), International Congress of Pharmacy and Health Sciences (FICPHS), International Congress of Chemistry and Pharmacy (FICCP). He is life member of Indian Pharmacy Graduate Association (IPGA), Association of Pharmaceutical Teachers of India (APTI), Indian Pharmacological Society (IPS).
Abstract:
According to the NNI, Nanotechnology is the understanding and control of matter at dimensions between approximately 1 and 100 nanometers (nm), where unique phenomena enable novel applications not feasible when working with bulk materials or even with single atoms or molecules. Nanotechnology is ideally suited for drugs with solubility problems .One of the key factors affecting bioavailability of drugs is solubility. Many bioactive natural products have poor water solubility .Nanotechnology has the potential to give new breath of life to those bioactive natural products which were rejected due to their poor solubility. Poorly soluble drugs can also be solubilized by other conventional methods. But those methods have various limitations. The nanocrystal technology enables formulations to be developed without the need of toxic surfactants (e.g. Cremophor EL) which may cause enhanced side effects or adverse reactions. Taxol formulation was very difficult because of hydrophobicity. It is made into liquid formula by dissolving it in a solvent called Cremophor. Many people react badly to this solvent and when administered intravenously experienced neurotoxicity. Abraxis, developed a form of Paclitaxel using nanoparticles of protein called Albumin. This protein can bind bind to receptors in blood vessels. Tumor response rate was twice as good for the nano formulated drug. Only 30 minutes are needed for a complete IV dose of the drug instead of three hours. Thus reduced side effects improved quality of life.
Fatimah Mohammad Budair
University of Dammam School of Medicine, Saudi Arabia
Title: Dermatosis due to Saudi cultural and religious practices that use complimentary and locally prepared remedies
Biography:
Fatimah Budair has completed MBBS from King Faisal University in Dammam ,Saudi Arabia in 2006 and finished the Saudi Board of Dermatology and Dermatological Surgery in 2011. She is a consultant of Dermatology in King Fahad Teaching Hospital of the University, University of Dammam and an assistant professor in Dermatology. She has published different papers in reputed journals and has been serving as a reviewer in the Saudi Journal of Dermatology and Dermatological Surgery.
Abstract:
Saudi Arabia, the largest Arab country of the Middle East, is defined by its Islamic heritage, and its Bedouin traditions. These rich family traditions and practices are handed down from generation to generation. Many of these socio-religious and cultural practices are done using complimentary and locally prepared remedies, but unfortunately these used remedies may not always be as safe as they seem, and cutaneous adverse reactions may occur. The lecture will discuss the habits practiced by people in Saudi Arabia using complimentary and popular natural remedies (henna, kohl, myrrh, honey and applying local remedies for treatment purposes ) and explain the potential secondary dermatomes that may develop, which can be unfamiliar to other doctors from different countries.
- Young Research Forum
Location: Location: Hyderabad International Convention Centre, India - See more at: http://pharmacognosy-phyto
Session Introduction
Srinu Meesala
IISER, India
Title: Isolation, purification, characterization of natural products from marine pseudomonas sps
Time : 17:25-17:40
Biography:
Srinu Meesala has completed his PhD from Andhra University and doing postdoctoral studies at IISER PUNE.
Abstract:
There is a perpetual need for new chemotherapeutants, especially novel antibiotics, to combat new diseases and drug-resistant pathogens that are becoming a significant threat to public health [1]. In spite of the development of chemi-informatics, molecular modeling and other rational drug discovery tools, natural products (NPs) still continue to be a major contributor to new drugs. Over 28% of the new chemical entities and 42% of the anticancer drugs introduced into the market in the last three decades can be traced back to NPs [2]. More than 50,000 microbial natural products (MNPs) have been obtained and have played an important role in drug discovery. However the rate at which new compounds are being discovered from microbial sources is declining. On the other hand, the emergence of severe resistance to antibiotics in microbial pathogens, such as Gram-positive methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant S. aureus (VRSA) increases, there is a need to discovery of MNPs with unique scaffolds to meet the urgent demand for new drugs. Phenazines are redox-active nitrogen-containing aromatic compounds produced by a diverse range of bacterial genera. we isolated Pseudomonas aeruginosa , Genbank accession no. KJ558373, producing phenazines, showing broad spectrum activity and exhibit strong cytotoxic activity against mouse macrophage (RAW 264), human neuroblastoma (SHSY5Y), human lung cancerous (A549), human liver cancer (HEPG2) cell lines with IC 50 value less than 50ng/ml. Chemical characterization studies like UV, IR, HRESIMS, 1D and 2D NMR revealed (1-2) are known phenazines and (3) is a novel phenazine derivative.
Anitha Vadekeetil
Panjab University, India
Title: Role of analytical, in silico and imaging tools in characterisation and activity studies of ajoene from garlic bulbs against biofilms of P. aeruginosa
Time : 17:40-17:55
Biography:
Anitha V completed M.Sc Biotechnology from Bharathidasan University followed by DBT sponsored Advanced PG Diploma in plant genetic engineering from Madurai Kamaraj University with distinction. Presently she is pursuing PhD from Panjab University, Chandigarh. She has published 3 research papers in international journals.
Abstract:
Biofilm of P. aeruginosa impose major health issues due to its resistance towards antibiotics and host immune responses. The postantibiotic era proposes urgent need for alternate strategies to combat biofilms. The relevance of dietary natural products is emerging in this context as they are safe to consume and have no side effects. However, the major concern in this area is the constraints in characterisation and analysis of herbal compounds because of lack of availability of standards and highly complex nature of these compounds. Hence, the present study focuses on applications of analytical, imaging and in silico tools for analysis and activity of ajoene, a novel compound from garlic (Allium sativum L.) bulb extract. Ajoene was extracted by TLC and showed 97.7 % purity by HPLC. Further characterisation was performed by H1 NMR and MS. The amount of ajoene in garlic extract was found to be 221.08 nmol/mg of extract by HPTLC. Results of TLC overlay, HPTLC and docking analysis elucidated the mechanism of action of ajoene against P. aeruginosa. Ajoene was found to exhibit antibiofilm effects which were confirmed by imaging techniques such as confocal laser scanning microscopy and scanning electron microscopy. Thus, the present study throws light on the possible techniques which could be helpful in evaluating the properties of herbal products.
Hina Deepak Mehta
Dadasaheb Balpande College of Pharmacy, India
Title: Milk thistle’s : A boon for multiple therapy
Time : 17:55-18:10
Biography:
Hina Deepak Mehta has completed her M.Pharm at the age of 25 years from rashtrasant tukdoji maharaj nagpur univesity. she is the assistant professor of Dadasaheb Balpande college of pharmacy, Besa Nagpur.
Abstract:
This article reviews on published reports pertaining to milk thistle’s (Sylibum marianum) benefits in the treatment of various diseases. This plant used from ancient times as a hepatoprotective drug. Along the hepatoprotective action of Silymarin, a flavonolignan from‘milk thistle’ was also effective in gastrointestinal disorders and its antitumoral activities, antioxidant, antiinflamatory agent, nephroprotector, radiation and skin protector. silymarin owns also other actions as an, anti-lipid peroxidative, antifibrotic,, immunomodulatory, liver regenerating, etc. It was also studied its neuro-psihiatric and cardiac action. A systematic review of this evidence to clarify what is known and identify gaps in knowledge would be important to guide design of future studies of the mechanisms of milk thistle and clinical trials.
Swastika Maity
Manipal college of Pharmaceutical Sciences, India
Title: Physico-chemical properties of ethno-medicinal plant Hygrophila auriculata and Paederia foetida leaves grown in herbal kitchen garden
Time : 18:10-18:25
Biography:
Swastika Maity is currently studying 4th year B.pharm from Manipal College of Pharmaceutical Sciences, Manipal University, Karnataka. She has presented poster and got the best poster award in Range Management Conference in Kalyani, West Bengal and Pharmaquora ,2015.
Abstract:
Medicinal plants have been used in pharmaceutical practices for thousands of years. India has a wide range of biodiversity and due to its tropical climate which can support large number of ethnomedicinal plant. Hygrophila auriculata and Paederia foetida are such ethno-medicinal plant. H.auriculata is an aquatic perennial herb with spine and P. foetida is a climber which has ovate to lanceolate leaves. As both the plant has wide medicinal values, people and tribes of local area of these plant use their leaves in their diet, the plants with root were collected from the marshy land of West Bengal and grown in herbal kitchen garden at Jhansi , which has semi arid tropical climate. Physical properties of leaves were measured, processed and analyzed for proximate principles as well as fiber fractions . Leaf length, width and petiole length was 6.60 ±0.17, 2.98± 0.18 and 1.86±0.19 cm for P. foetida and 5.49±0.17, 1.21±0.11 and 0.49±0.11 cm for H. auriculata. Dry matter of leaves was 23.04 and 16.98%, in the leaves of P. foetida and H. auriculata. On dry matter basis, nitrogen content found to be 2.12 and 4.11% with calculated crude protein 13.24 and 25.67%, in the leaves of P. foetida and H. auriculata. ADF , NDF , lignin, were 29.30, 39.23 and 9.81%, in P. foetida leaves and 16.89, 28.95 and 4.68%, in H.auriculata leaves. Ethar extracted oils was 5.95 and 12.28%, in the leaves of P. foetida and H. auriculata. Ash content which mainly constitute the minerals were 9.93 and 20.55% in P. foetida and H. auriculata., Nutritional values of H. auriculata leaves found better than P. foetida leaves with more protein, minerals, essential oils with less lignin for better digestability in the system. However, total dry matter and organic matter found more in P. foetida leaves. Thus, both P. foetida and H. auriculata , ethnomedicinal plant can be grown in small kitchen garden maintaining their well established nutritional quality and daily use in diet as medicinal plant.
Biography:
Will be uploaded soon.
Abstract:
Mung bean is rich source of protein, vitamins and certain minerals. Mung bean grains also exhibits a stronge antioxidant activity. Vitexin and isovitexin are the major compounds responsible for the antioxidant activity of mung bean. Due to their clinical importance, there is a need to develop a simple, sensitive and accurate methods for the determination of these compounds. Thus an attempt has been made to devolep a reversed-phase high-performance liquid chromatography method for the simultaneous determination of Vitexin and Isovitexin present in mung bean. The chromatographic system was optimized using an analytical Hypersil ODS column (150 x 4.6mm) with mobile phase 0.1% ortho-phosphoric acid (mobile phase A) and acetonitrile (mobile phase B) at flow rate of 1.0ml/min. Vitexin and Isovitexin were eluted at 9.52 and 10.25min and the chromatograms were extracted at 360.0nm. The method was validated for linearity ranges, precision, recovery, limit of detection (LOD) and limit of quantification (LOQ). The linearity for both compounds were observed from 5ppm to 100ppm with correlation coefficient coffeicient of 0.999, recovery for vitexin and isovitexin was 98.27 and 97.60%, respectively. The %RSD for interday precision (vitexin 0.98% and isovitexin 1.20%) and intraday precision (vitexin 1.42% and isovitexin 1.73%) was found to be satisfactory. The LOD values were 0.094 and 0.069mg/100g, while the LOQ were 0.312 and 0.230mg/100g, for vitexin and isovitexin, respectively. The developed and validated method was successfully applied for the detection and quantification of vitexin and isovitexin content present in two mung bean samples procured from different regions.
K Sai sruthi
Sri Padmavathi Mahila Visvavidyalayam, India
Title: Pharmacognostic and physicochemical studies of the roots of stereospermum suaveolens
Time : 18:40-18:55
Biography:
Sai sruthi completed her B.Pharmacy from C.L. Baid Metha College of Pharmacy, Chennai and M.Pharmacy from Vishwabharathi College of Pharmaceutical sciences, affiliated to JNTUK. I am pursuing my Ph.D from Sri Padmavati Mahila Visva Vidyalayam as a full time research scholar. I had attended one international and five national conferences.
Abstract:
The aim of the present study is to evaluate pharmacognostical parameters of roots of Stereospermum suaveolens which includes macromorphology, microscopical characters, physico-chemical constants and phytochemical screening. Roots of Stereospermum suaveolens were collected from Tirumala hills, Chittoor district, A.P and authenticated by the botanist Dr. Madhava chetty, Herbarium keeper, Department of Botany, Sri Venkateswara University, Tirupati, India and a specimen has been deposited in Department of Botany, Sri Venkateswara University, Tirupati, India. Roots were washed thoroughly, made into small pieces, shade dried and powdered in a wiley mill. The fresh root was used for the study of macromorphological and microscopical characters; whereas the dried root powder was used for determination of powder microscopy, physicochemical characterization and phytochemical screening. The dried plant material was subjected for determination of physicochemical parameters such as total ash value, acid insoluble ash, water soluble ash, alcohol soluble extractive, water soluble extractive value, pH, moisture content, foreign matter present, foaming index, etc. Fluorescent analysis and preliminary phytochemical studies had been carried out. Physicochemical parameters had shown characteristic values such as total ash value of 4.5%, acid insoluble ash of 1%, moisture content 16.5% and alcohol soluble extractive 8.8%. Preliminary phytochemical studies showed the presence of flavonoids and saponins. This information will assist in standardization for quality, purity and sample identification and further it will be used for pharmacological and therapeutical evaluation of the species.