Maninder Meenu
AcSIR, India
Title: A simple RP-HPLC Method development and validation for the simultaneous determination of vitexin and isovitexin present in Mung bean (Vigna radiata L.)
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Biography: Maninder Meenu
Abstract
Mung bean is rich source of protein, vitamins and certain minerals. Mung bean grains also exhibits a stronge antioxidant activity. Vitexin and isovitexin are the major compounds responsible for the antioxidant activity of mung bean. Due to their clinical importance, there is a need to develop a simple, sensitive and accurate methods for the determination of these compounds. Thus an attempt has been made to devolep a reversed-phase high-performance liquid chromatography method for the simultaneous determination of Vitexin and Isovitexin present in mung bean. The chromatographic system was optimized using an analytical Hypersil ODS column (150 x 4.6mm) with mobile phase 0.1% ortho-phosphoric acid (mobile phase A) and acetonitrile (mobile phase B) at flow rate of 1.0ml/min. Vitexin and Isovitexin were eluted at 9.52 and 10.25min and the chromatograms were extracted at 360.0nm. The method was validated for linearity ranges, precision, recovery, limit of detection (LOD) and limit of quantification (LOQ). The linearity for both compounds were observed from 5ppm to 100ppm with correlation coefficient coffeicient of 0.999, recovery for vitexin and isovitexin was 98.27 and 97.60%, respectively. The %RSD for interday precision (vitexin 0.98% and isovitexin 1.20%) and intraday precision (vitexin 1.42% and isovitexin 1.73%) was found to be satisfactory. The LOD values were 0.094 and 0.069mg/100g, while the LOQ were 0.312 and 0.230mg/100g, for vitexin and isovitexin, respectively. The developed and validated method was successfully applied for the detection and quantification of vitexin and isovitexin content present in two mung bean samples procured from different regions.
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