Day 2 :
Keynote Forum
M J Nanjan
JSS College of Pharmacy, India
Keynote: The role of natural products in drug discovery
Time : 10:00-10:25
Biography:
Will be uploaded soon.
Abstract:
Despite the tremendous success of chemo- and antibiotic therapy, it is believed that only 30% of about 2000 existing diseases can be cured today. Others can be treated only symptomatically or insufficiently and some not at all. There is an urgent need, therefore, to discover efficient and causality acting drugs to treat several diseases. In this connection, Lipinski’s rule of 5 has created awareness among medicinal chemists, namely addressing pharmacokinetic properties early in the drug discovery process is of vital importance for discovering newer drugs. Natural products, however, are an exception to this rule. Unlike synthetics, they have more stereogenic centers and architectural complexity. In addition, they contain more C, H and O and less N and other elements compared to synthetics. Their molecular weight is generally higher than 500. They have higher polarities and greater water solubility. Rule of 5 is, therefore, not applicable to natural products. rnCombinatorial chemistry, a collection of methods whereby simultaneous chemical synthesis of a large number of compounds using a variety of starting materials can be produced quickly and high throughput screening, a process whereby the large number of compounds can be tested against biological targets to find active compounds, have been used as drug discovery source for the past more than 30 years. Since 1980 onwards a great number of screening programs have been initiated by industries, universities, and research institutions, searching for new bioactives. But then, the number of new chemical entities using these techniques was only 25 in the year 2005 and it is declining since then. And what is more, to date we find only one new chemical entity, namely, Sorafenib from Bayer, approved as an anticancer drug, resulting from these techniques. In other words, the expected surge has not materialized.rnNatural products, however, have been closely linked through the use of traditional medicine for thousands of years. Clinical, pharmacological and chemical studies of these traditional medicine derived predominantly from plants were the basis of early medicines like morphine, quinine, digitoxin, etc. After the discovery of penicillin by Fleming in 1928, reisolation and clinical studies by Chain and coworkers in early 1940s, commercialization of synthetic penicillins revolutionized drug discovery approaches. Today a significant number of natural product drugs/ leads are also produced from microbes. The functional group diversity and architectural platforms built into natural products during biosynthesis, have been teaching us lessons about the chemical functionality that is compactable with the aqueous milieu of the biological environment. Chemomics, understanding the molecular logic of biosynthetic enzymes and pathways, has opened up new approaches to reengineer newer natural products.rnAlthough natural products have played an important role in drug discovery, in the past few years big pharmaceutical companies have either terminated or considerably scaled down natural product operations. But then, when one analyzes the data on drugs and new chemical entities developed for all diseases and from different sources the world over for the past nearly 30 years, it shows that less than 30% of them are of synthetic origin. This clearly demonstrates the influence of natural products in drug discovery. Nature, in one form or another, seems to continue to serve as a source of novel active agents that will serve as leads and scaffolds for developing the drugs desperately needed for the multitude of diseases. The inventory of natural molecules remains incomplete. Discovery of new molecules and their functions are likely to continue as sources of natural products are more systematically evaluated. rnIn this connection, a multidisciplinary approach for drug discovery, involving the generation of truly novel molecular diversity from natural product sources combined with total and combinatorial synthetic methodologies including the manipulation of biosynthetic pathways, should provide the best solution to the current productivity crisis facing the scientific community engaged in the drug discovery and development. It is, therefore, necessary that we should expand and not decrease the exploration of nature as a source of novel active agents that may serve as leads and scaffolds for elaboration into the desperately needed efficacious drugs for the multitude of disease indications.rn
Keynote Forum
KN Sunil Kumar
SDM Centre for Research in Ayurveda and Allied Sciences, India
Keynote: Sophistication of fingerprinting methods for polyherbal traditional medicines from ‘mere quality’ to ‘quality plus composition’
Time : 10:25-10:50
Biography:
KN Sunil Kumar has B.Sc. M.Sc. and PhD in Pharmacognosy. He worked as Senior Research Fellow in an ICMR project ‘Quality Standards of Indian Medicinal Plants’ to work on monographs for 46 medicinal plants subsequently published by ICMR as book ‘Quality Standards of Indian Medicinal Plants’. He is Principal Investigator of four major research projects on standardization of Ayurvedic formulations sanctioned by AYUSH, UGC, VGST and RGUHS. He is author of 49 research papers on the theme of standardization of medicinal herbs and their products. He is serving as Chief Editor of ‘Journal of Ayurvedic and Herbal Medicine’; Associate Editor for ‘The Journal of Phytopharmacology’; Executive editor of ‘Research and reviews: Pharmacognosy and Phytochemistry’, subject expert in the specialty of Pharmacognosy for ‘AYU – An International Quarterly Journal of Research in Ayurveda’ and editorial member several other journals. He is life member of ‘Society of Pharmacognosy’ and recipient of ‘Dr. PD Sethi award’ for 5 best HPTLC papers.rn
Abstract:
Polyherbal traditional medicines (PTMs) are important category of traditional medicines (TMs) practiced in India. Based on therapeutic applications ancient books of Ayurveda prescribe several polyherbal combinations such as Triphala, Dashamoola etc. The composition of the formula is based on some Ayurvedic principles on properties of the individual ingredients. There are several such formulae mentioned in Ayurvedic Formulary of India (AFI), but very few having monographs on its quality standards published in Ayurvedic Pharmacopoeia of India (API). The ones with monograph in API needs sophistication of the specified standards as the data included are quite insufficient to have exact idea of its chemical composition. Also, in the present context of increasing demand for alternative therapies, standardization of herbal medicines employing more apt fingerprinting techniques are need of the hour towards globalization. In this study, an Ayurvedic PTM namely Jambupallavadi Kvatha Churna (JKC) of AFI used traditionally for chardi (emesis), pravahika (dysentery), meha (diabetes) is considered for deriving fingerprints of its standards employing the usual pharmacopoeial analytical tests in combination with certain chromatographic fingerprints which are not strict pharmacopoeial test for PTMs. Macroscopical and physico-chemical constants (loss on drying at 105º, total ash, acid insoluble ash, ethanol and water soluble extractive) of ingredients (Jambupallava, Amrapallava, Usheera, Vata, Asvattha) were calculated and JKC prepared by mixing equal proportions of the five ingredients were also analyzed for the above physico-chemical constants along with HPTLC and GCMS fingerprinting along with ingrdients. The combination of the results obtained by amalgamation of the currently pharmacopoeial and the non-pharmacopoeial fingerprints would serve in updating the current structure of monograph preparation of PTMs.
- Track 1: Advanced trends for the future of herbal drugs and its products
Track 5: Toxicology Studies of Plant Products
Track 6: Ethnopharmacology
Track 7,8: Phytochemistry and Phytoconstituents, Industrial Pharmacognosy
Location: Hyderabad International Convention Centre, India
Session Introduction
Gideon O Alade
Niger Delta University, Nigeria
Title: Antifertility activity of Bambusa vulgaris aqueous leaf extracts in male wistar rats
Time : 11:05-11:25
Biography:
Will be uploaded soon.
Abstract:
Globally, there is a need for family planning as a result of increasing human population growth rate with its undesirable impact on the environment, economic growth and poverty in several parts of the world most especially in the underdeveloped countries. Ninety percent of the world’s contraceptive users are women, probably due to the slow progress in development and limited possibilities of male contraceptives. Several medicinal plants have been implicated as antifertility in males, but only a few have been investigated. Bambusa vulgaris L. (Poaeceae), commonly known as “Bamboo” is found in Tropical and Sub-tropical areas It is commonly referred to as ‘Oparun’, ‘Iko’, and ‘Atosin’ among the Yoruba, Bini and Igbo tribes of Nigeria respectively. Ethnobotanical study of antifertility medicinal plants used by the people of Kathijavadi village in India revealed that the extract of B. vulgaris leaves is taken orally to reduce sperm count. Therefore, the study seeks to provide scientific justification to the acclaimed sperm reducing potentials of B. vulgaris leaf in male wistar rats. Male wistar rats were administered with 250 (n=12) and 500 mg/kg (n=24) of B. vulgaris leaf ethanol extract for 14 and 28 days, distilled water acting as negative control and 6 rats each were sacrificed at the end of 14 and 28 days separately. They were evaluated for sperm concentration, motility, testosterone (T), leutinizing (LH) and follicle stimulating hormones (FSH), histology of the testes was also carried out. The remaining of the rats administered with 500 mg/kg for 28 days were kept and allowed free access to feed and water while extract administration was withdrawn. Six rats were sacrificed weekly and sperm concentration and motility were evaluated for probable reversal of activity. There was 42 and 31 % reduction in sperm count at 14 and 28 days respectively in rats administered with 250 mg/kg while at 500 mg/kg dose, the percentage reduction in sperm count was 60 % and there was almost a complete reversal of activity 14 days after cessation of treatment. The result justified the ethnobotanical claim of the use of B. vulgaris leaf in reducing sperm count.
Prasad Thakurdesai
Indus Biotech Private Limited, India
Title: Therapeutic efficacy of trigonelline-based standardized extract of fenugreek seeds on Levodopa induced dyskinesia in 6-OHDA lesioned rat model of Parkinson’s disease
Time : 11:25-11:45
Biography:
Prasad Thakurdesai is GM- Scientific affairs at Indus Biotech Private Limited, Pune, India since 2008. He completed his PhD in 2001 from Nagpur University, Nagpur, India. He has experience of 17 years in academic institutions and 9 years in pharmaceutical industries. He has guided 2 PhD and more than 30 M.Pharm. research students. He has published more than 77 articles in International and National Peer reviewed Journals, presented 49 papers in international conferences. His research interests include Pharmacology, Toxicology, Nutraceuticals and health related Quality of life (HRQoL).
Abstract:
The objective of the present work is to explore the therapeutic efficacy and possible mechanism of trigonelline-based standardized extract of fenugreek seeds (IBHB) on L-dopa induced dyskinesia (LID) in 6-hydroxydopamine (6-OHDA) lesioned rat model of Parkinson’s disease (PD). The motor symptoms of PD and LID were induced in rats with single unilateral intracerebroventricular injections of 6-OHDA in the striatum area of brain (PD) on day-0 and daily treatment with L-DOPA + Benserazide from Day-29 to Day-56 (LID) respectively. Rats were then randomly divided into groups and administrated with subacute treatment either with vehicle, amantadine (40 mg/kg, i.p.) or IBHB (15, 30 or 60 mg/kg, oral) for next 45 days and then sacrificed for collection of brain striatal samples for ex vivo experiments. Subacute treatment of IBHB showed dose-dependent reduction in behavioral parameter scores (abnormal involuntary movements (AIMS), grid test, catalepsy bar test, Forelimb adjusting steps) as compared to vehicle treated LID group. During ex vivo experiments, IBHB showed restoration of upregulated neurotransmission (serotonin and dopamine turnover and 3-O-Methyldopa levels), immunoreactivity (FosB and 5HT2c), and mRNA expressions (c-FOS, CO-1, Homer-1, Parkin, Pdyn, Penk and PINK1) and downregulated mitochondrial respiratory chain complexes activity and mRNA expression of JunD in striatum as compared to vehicle treated LID control rats. In conclusion, subacute treatment of IBHB showed promising therapeutic anti-dyskinetic efficacy in preclinical model of LID in rats and supports the utility for the improved treatment of LID and PD.
Swaroopa Rani N Gupta
Brijlal Biyani Science College, India
Title: Polarographic study of meta-hydroxy acetanilide and its determination
Time : 11:45-12:05
Biography:
Swaroopa Rani N Gupta has done PhD in Chemistry from Nagpur University, Maharashtra, India in 1993. She is an Associate Professor in the Department of Chemistry, Brijlal Biyani Science College, Amravati, Maharashtra, India. She has published more than 15 papers in reputed international journals. She has presented papers at international conferences at India, Singapore, London, Dubai, Hong Kong, Mauritius, Tashkent and has been serving as Technical Committee Member of international conferences at Singapore, UK, Dubai, Hong Kong, Mauritius, Korea, Turkey, New Zealand, etc.
Abstract:
Present paper deals with polarographic study of effect of maxima suppressors and supporting electrolytes on anodic waves of meta-hydroxy acetanilide and polarographic determination of meta-hydroxy acetanilide under optimum concentration of maxima suppressors and supporting electrolytes. The polarographic method has been developed to study qualitatively the effect of maxima suppressor (fuchsin) and supporting electrolyte (nitric acid) on oxidation wave of meta-hydroxy acetanilide. Polarograms of system were recorded from 500 to 1300 by using Rotating Platinum Micro Electrode as anode and Saturated Calomel Electrode as cathode on DC Recording Polarograph using Omniscribe recorder. It shows a similar behavior to that observed for paracetamol. It produces anodic wave at rotating platinum electrode. The oxidation yields the 3-N-acetylaminosemiquinone and represents a reversible reaction. Polarographically a value of 900 mV is found for decomposition potential of meta-hydroxy acetanilide. Wave analysis point to one electron-step for each wave. Waves of meta-hydroxy acetanilide are only proportional to concentration at low concentrations.
B Janarthanam
Poonga Biotech Research Centre, India
Title: Studies on phytochemical analysis, proximate analysis and antioxidant activity of Cucumis melo seed extract
Time : 12:05-12:25
Biography:
B Janarthanam has completed his PhD degree in the subject of Botany from University of Madras. He has also worked as Chief Scientist & Project Principal Investigator in OMNI GREEN Organic Bio Park Private Limited, as Project Co-PI & Research Associate in PG & Research Department of Botany, Pachaiyappa’s College, Chennai and as Project Co-PI & Junior Research Fellow in Shri A M M Murugappa Chettiar Research Centre, Tharamani, Chennai. He has more 10 years of research experience in various fields of Secondary Metabolite production, Enzyme assays, Antioxidant activity and plant tissue culture techniques. He has served as Project Principal Investigator & Co-Principal Investigator in projects sponsored by Department of Science and Technology, New Delhi, National Medicinal Plant Board, New Delhi and M/s Shrivathsa Herbs & Species Pvt. Ltd. He has guided more than 40 project students from various colleges and Universities for their BTech/MSc/MTech dissertation work. He has 18 international scientific publications in reputed journals and has participated and presented his research findings in more than 25 international and national conferences. He has also given oral presentations as Invited Speaker in the “Asia Pacific Conference of Plant Tissue Culture and Agribiotechnology” (APaCPA 2007) held in Malaysia and in the “International conference on Natural Products and Biomedical Technology”, ICNPBT 2011, at Annamalai University.
Abstract:
The present study was performed to investigate the phytochemical screening, proximate composition, total phenol, total flavonoid content and DPPH assay of seed extracts of Cucumis melo. Phytochemical screening of various extracts such as aqueous, ethanol, chloroform, acetone and petroleum ether of seed extracts, revealed the presence of tannins, saponins, phenols, flavonoids, cardiac glycosides, terpenoids, alkaloids and steroids. Butylated Hydroxy Toluene (BHT), Gallic acid (GA) and Quercetin (Q) were taken as standard in case of antioxidant activity, phenol and flavonoid content respectively. Total phenol and flavonoid contents were quantitatively estimated. Total phenolic content was estimated by Folin–Ciocalteau method and flavonoid content measured by Aluminum chloride method. Proximate analysis such as total protein, carbohydrate, ash, crude fibre and moisture contents were carried out following standard methods. The seed extracts were evaluated for antioxidant activities by DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity. Among five different solvents used, maximum antioxidant activity was found in aqueous extract (73.2%) followed by others. The total phenol and flavonoid content in seed extract were found to be 23.32 mg gallic acid equivalents (GAE)/g and 63.1 mg Quercetin Equivalent (QE)/g, respectively. The aqueous extracts from dry powdered seed of Cucumis melo had superior level of antioxidant activity. The powerful antioxidant effect is attributed to the greater amount of phenols and flavonoids compound in the ethanolic peel extracts of Cucumis melo.
Juliao A Monjane
Eduardo Mondlane University, Mozambique
Title: Phytochemical investigation of Cadaba natalensis roots
Time : 12:25-12:45
Biography:
Juliao A Monjane is a PhD candidate at Lund University in Sweden. He is currently in the 3rd year of PhD studies. His research project is related with isolation and characterization of compounds with interesting biological activities derived from selected Mozambican medicinal plants used locally in antibacterial therapy. He teaches courses for Bachelor’s students at Eduardo Mondlane University in Mozambique. His research interests are focused on areas related with Natural Products, Organic Synthesis, and Nuclear Magnetic Resonance Spectroscopy.
Abstract:
The roots of Cadaba natalensis (Capparaceae family), known as Tssatssassana or Ndoda Ya Munhama in Southern Mozambique have been used to treat tuberculosis. Several species of the genus Cadaba have been studied phytochemically, although no previous studies have been carried out on C. natalensis. The aim of this work was to isolate and characterize some phytochemical constituents present in the roots of this plant. The isolation of the compounds was done by chromatographic methods and two novel compounds together with five known compounds were isolated. The novel compounds were by spectroscopic techniques characterized as dimeric a tetrahydroisoquinoline similar to thalifoline and 4-oxazolidine derivatives, and a 2-oxazolidine derivative that for the first time was obtained from a natural source. This is the first study in which the rare five membered ring structures (2 and 4-oxazolidine derivatives) are isolated from a plant, although similar compounds have previously been isolated from marine sources. Beside these three compounds, also thalifoline, N-methylcorydaldine, β-sitosterol, and nepetin were obtained. The 2 and 4-oxazolidine derivatives have been reported to possess antimicrobial, antibacterial, and anticancer activities, while tetrahydroisoquinoline derivatives can have vasorelaxant activity. The structures of isolated compounds were characterized by 1D and 2D NMR spectroscopy, HRESI-MS, and by the comparison with the reported spectroscopic data for the known ones.
Dharmesh R Chejara
University of the Witwatersrand, South Africa
Title: Natural polymer based novel micro porous soft hydrogel system: A potential viscosupplimentation for biomedical applications
Time : 12:45-13:05
Biography:
Dharmesh R Chejara has completed his PhD at the age of 29 years from Academy of Scientific and Innovative Research (AcSIR), New Delhi and PhD research work was carried out at CSIR-CSMCRI, Bhavnagar, Gujarat, India. He is doing his postdoctoral research since July 2014 from University of Witwatersrand, Johannesburg South Africa. He has published more than 10 papers in reputed journals
Abstract:
We report herein, synthesis of natural polymer sodium alginate (Na-Alg) based novel soft hydrogel system where aminocaproic acid (ACA) was attached on to the polymer using carboddimide coupling chemistry. The designed soft hydrogel was characterized using various analytical techniques such as Fourier transform infrared spectroscopy (FT-IR), Nuclear magnetic resonance spectroscopy (NMR), Scanning electron microscopy (SEM) and Differential scanning calorimetry (DSC). This soft hydrogel was further investigated for stress responsiveness using different rheological studies which confirmed the thixotropic nature of the gel, which is rendered flowable (fluid) on applying physical stress and recovers its ”rigid” gel structure upon removal of these applied stimuli. In addition the hydrogel was found to be nontoxic in nature. This approach of synthesizing a natural polmer-based materials could be applicable to a broad variety of other natural polymers and may have a potential utility as a viscosupplimentation for orthopedic implants in the field of biomedical applications.
Ciddi Veeresham
Kakatiya Univeristy, India
Title: Development and Evaluation of selected Indian medicinal plant based poly herbal Anti-Aging cream
Biography:
Will be uploaded soon.
Abstract:
Practice of using herbal remedies as a strategy against aging skin has been evolved as an imperative step to accomplish comprehensive skin rejuvenation. Present study is aimed to prepare and evaluate a poly herbal cream containing standardized extracts of Aloe vera (A), Ocimum sanctum (O) and Punica granatum peel (P) at different ratios. Santalum album, Citrus aurantium, Olea europa, Prunus amygdalus oils, Cetyl alcohol and Stearic acid were used in a ratio of 0.1:0.1:1.0:0.5:0.5 and 3.0 respectively, along with preservatives to formulate a cream base for formulation water in oil (W/O) creams i.e. F1 to F6. Prepared formulations were evaluated for formulation parameters like pH, viscosity, spreadabilty, extrudability and stability. Further, formulations were also evaluated for anti-oxidant, anti-microbial and advanced glycation end product inhibition (AGE) properties. Formulations F3-F6 have shown no phase separation upon storage. Formualtions F3 and F6 have featured lower spreadability and extrudabiltiy. The order of anti-oxidant ability of the all formulations found as F6>F5>F4>F3>F2>F1. Formulations F4 and F5 shown comparable AGE inhibitory and anti-microbial effects to standards Aminoguanidine and Neomycin sulphate cream, respectively, however F4 shown higher AGE inhibitory effect vis-a-vis with F5. In human volunteer’s study, the formulations F4 and F5 shows no redness, edema, inflammation and irritation during irritancy studies. The synergistic effects of formulation F4 containing A: O: P (each 1.6% w/w) has addressed hyperpigmentation, erythema, wrinkles and firmness of skin more significantly than F5. These finding indicate the significant anti-aging potential of examined poly herbal anti aging cream.
P Subramanian
Drug Standardisation Unit, India
Title: Pharmacognosy & physico – chemical evaluation of homoeopathic drug erigeron canadensis linn
Time : 13:35-13:55
Biography:
Will be uploaded soon
Abstract:
Herbal products are composed of many diverse chemical constituents of immense therapeutic value and capable of variation. Standardisation is therefore extremely essential and mandatory for global acceptance. Erigeron canadensis Linn., is an erect annual herb belonging to the family Asteraceae. It is also known in English as ‘Canada Fleabane’. Aerial parts are used in homoeopathy for bruises, cough, dysuria, gonorrhoea, haemorrhages, haemorrhoids, spermatorrhoea and wounds. The leaves are 2.5 – 4 cm long, sessile, linear obovate with rough surface. Stems are 2 to 5 mm thick, ridged with coarse surface and densely hairy. Epidermal cells in surface possess wavy to sinuate sides; stomata are anomocytic, anisocytic and tetracytic; trichomes are uniseriate conical in structure. The midvein in transection is flat on adaxial and ribbed on abaxial. Palisade is 2- layered. A secretory cavity is present beneath the central vascular bundle. Stem in transection is rounded and undulated, covered by uniseriate conical hair. The vascular tissue is made of several V. bundles in a ring capped by sclerenchyma sheath. Centrally pith is present with a disintegrated lumen. Mature stem shows outermost epidermis replaced by cork. Secondary xylem is well developed and phloem is reduced. A vascular cambium is present. Pollen grain is a monad, prolate spheroided or sub prolate with spinescent surface. The powder microscopic and organoleptic characters are presented. Physico – chemical parameters of raw drugs viz., extractive values, ash values, formulation besides wt.per ml., total solids, alcohol content along with HPTLC fingerprinting and UV studies were undertaken of the mother tincture for the first time.
P Padma Rao
Drug Standardisation Unit, India
Title: Pharmacognostic standardisation of rumex crispus linn. in homoeopathic
Time : 13:55-14:15
Biography:
Will be uploaded soon
Abstract:
Plant kingdom represents an extraordinary reservoir of molecules, that can be beneficial to mankind. Rumex crispus L., is a perennial shrub belonging to the family Polygonaceae. It is also known in English as ‘Curled dock’ or ‘yellow dock’. It is a native of Europe and also occurs worldwide. The roots are useful in various therapeutic conditions viz., abortion, asthma, bronchitis, catarrh, corns, coryza, cough, and diarrhoea. The roots are 0.5 to 1.3 cm thick, blackish, brown, tortuous with irregular striations and with long, wiry rootlets. The outermost phellem is 8-10 layered, shrivelled and discontinuous. Phelloderm occurs in two zones with outer possessing elongated cells in undulated layers containing dense resinous matter and sphaeraphides. Secondary phloem is upto 25 celled and interrupted by 1-3 seriate medullary rays. The central cortex is extensive and traversed with vascular strands. Pith is present at the centre. The powder microscopic and organoleptic characters are presented. Physico – chemical parameters of raw drugs viz., extractive values, ash values, formulation besides wt.per ml., total solids, alcohol content along with HPTLC fingerprinting and UV studies were undertaken of the mother tincture for the first time.
Chittipong Tipbunjong
Mahidol University, Thailand
Title: Enhancement of C2C12 myoblast proliferation and differentiation by diarylheptanoids form Curcuma comosa Roxb
Time : 14:15-14:35
Biography:
Chittipong Tipbunjong is a Ph.D. candidate at the Department of Anatomy, Faculty of Science, Mahidol University. He receives a scholarship from the Office of the Higher Education Commission. At 2013-2014, he was a visiting scholar student at the Department of Physiology, College of Medicine, University of Kentucky.
Abstract:
Enhancement of C2C12 myoblast proliferation and differentiation by diarylheptanoids form Curcuma comosa Roxb.: Diarylheptanoids is a phytoestrogen that exhibits numerous biological activities. However, its effect on myogenesis is not known. Previously, it has been reported that estrogen improved muscle regeneration after injury and that mouse myoblasts, C2C12, expressed estrogen receptors. This study, therefore, aims to determine the effect of 1-(4-hydroxyphenyl)-7-phenyl-(6E)-6-hepten-3-one, which is extracted from Curcuma comosa Roxb. and has an estrogenic activity comparable to that of genistein, on myoblast differentiation. Treatment with this compound increased the expressions of myogenin and myosin heavy chain (MHC) proteins determined by western blotting and immunofluorescence staining. These effects were blocked by ICI 182,780 and ER-ï¡ siRNA suggesting that ER-ï¡ is involved in the enhancement of myoblast differentiation. At the early stage of differentiation, phosphorylated ERK, Akt and p38MAPK were increased. However, blockades with their specific inhibitors showed that only phospho-Akt and phospho-p38MAPK, but not phospho-ERK, were associated with myoblast differentiation. The compound also increased phosphorylation of P70S6Kinase, GSK3-β, β-catenin, and NF-ĸB. Interestingly, blockades with Akt inhibitor, Rapamycin, and SB203580 revealed mTOR/P70S6Kinase and GSK3-β/β-catenin are the downstream of Akt, but NF-ĸB is under the p38 MAPK and no crosstalk between Akt and p38 MAPK during differentiation. It is concluded that the diarylheptanoid enhances C2C12 myoblast differentiation by increasing MHC and myogenin levels through ER-α mediated Akt/mTOR/P70S6Kinase, Akt/GSK3-β/β-catenin, and p38 MAPK/NF-ĸB signaling.
Ashok Surana
Andhra University, India
Title: Leaders in holistic healing and body therapeutics
Time : 14:35-14:55
Biography:
Ashok has completed Bachelorette of Engineering from JNTU, Andhra University and is a alternative medicine enthusiast. He has been researching the healing properties of spices and herbs for more than fifteen years now. The dominant idealogy stemmed from the famous adage – live and let live. If we dont overwhelm our bodies with chemicals and let let it heal itself, it has the intelligence to keep the harmful bacteria at bay.
Abstract:
I have studied the medicinal effect of one spice taken at a time on the body methodically and found that when these spices are taken in a specidied quantity, at a particular time with attention given to how they are taken, it can have wondrous effects on the health of an individual. Example cow ghee. It is a super barrier against all forms of bacteria, for the gums and the intestines. Mustard oil is for the sinus what blood is for the body, the ultimate protector. Complicated management of dust allergy is manageable in the simplest of ways by getting mustard oil to trickle in the sinovial cavities. Clove is the master rejuvenator, the antipyretic. When you feel run down, clove puts you back into action. Cardamom the body to retain more moisture in the intestines. That is good bye to hard stools. Fennel seeds is the natural brush for the intestines. Like a tooth brush, fennel also needs to be used judiciously. Gum arabica / edible gum is the spice to look to when we need slowing down of flow of food through the intestines for ensuring better absorption of nutrients. Basically, giving power back to the body for healing ensures the problem gets solved without any side effects and from the root.
Anil Kumar Chintaluri
GITAM University, India
Title: Antimicrobial activity of essential oils
Time : 14:55-15:15
Biography:
Anil Kumar Chintaluri Completed M.Sc., (Microbiology) from Acharaya Nagarjuna University, Guntur and M.Phil., from Andhra University, Visakahpatnam. Presently working as Sr. Asst. Professor, Dept. of Microbiology, Vizianagaram and Pursuing Ph.D from GITAM University, Visakhapatnam. I have Published 4 research papers in reputed International Journals. My area of research is on Antimicrobial activities of essential oils and Plant extracts & TLC – Bioautography.
Abstract:
Antimicrobial activity of essential oils.
Sandeep Arora
Chitkara University, India
Title: Novel bioactive semi-synthetic nitric-oxide releasing derivatives of resveratrol
Time : 15:15-15:35
Biography:
Will be uploaded soon.
Abstract:
Nitric Oxide is involved in a multitude of physiological and pathophysiological states in mammals. For example, it is now known to be involved in the control of blood pressure neurotransmission, and in the immune defense system of the body. Nitric Oxide has low solubility in water and is unstable in the presence of various oxidants. It makes it difficult to introduce as such into biological systems in a controlled or specific fashion. Therefore, development of chemical agents that release NO is important. Numerous compounds are available which show anti-inflammatory, analgesic, anti-ulcer, antimicrobial agents and antihyperlipidemic activity. Resveratrol is a natural compound reported to possess a broad spectrum of biological activity with less side effects. Due to its wide range of biological activity, resveraterol has received a considerable interest in the field of drug discovery and therefore it constitutes a relevant synthetic target in Pharmaceutical Industry. A series of resveratrol derivatives were schemed by treating with chloroalkanols or chloroacetic acid then with Nitric oxide donor group for nitrate introduction by treating alkylated compounds with a mixture of fuming nitric acid and acetic anhydride in chloroform in cold conditions to get the final compounds. The purity of the compounds ascertained by TLC in different solvent systems and the structures of the synthesized compounds confirmed by their elemental analyses, proton NMR, IR and mass spectral data. All the title compounds further put in process for screening for biological activity.
Anoja Megalathan
University of Sri Jayawardenepura, Sri Lanka
Title: Natural Turmeric enapsulated Layered Double Hydroxides as Anti-microbial Nanohybrids
Time : 15:35-15:55
Biography:
Anoja Megalathan has completed her under graduate degree at the age of 24 from Institute of Chemistry, Ceylon, Sri Lanka. She has a business start up on natural and healthy beverage company named ‘Aqua Sizzle’ and also worked as research scientist at Midaya Ceramics, Sri Lanka; research intern at the national University of Sri Jayawardenepura, Sri Lanka
Abstract:
Turmeric (Curcuma longa) has been long recognized for its anti-inflammatory properties against a wide variety of conditions. However, it’s practical applications have been limited due to the instability under thermal and UV radiation. There had been only few attempts to stabilize turmeric. There-fore, this study focuses on exploring the potential of nanotechnology based approaches to stabilize the natural turmeric and to evaluate the antimicrobial activity of the resulting nanohybrids. In this study, natural turmeric has been encapsulated into nano layers present in Mg-Al-Layered double hydroxides (LDHs) using a simple co-precipitation method. The suggested method avoids any isolation of active compounds, i.e. curcumin from natural turmeric. The LDHs selectively encapsulate curcumin during the synthesis process. Powder X-ray diffraction (PXRD) and Fourier transform infrared (FTIR) characterization confirmed the successful synthesis and encapsulation of curcumin into LDH. Thermogravimetric data and UV stability data confirmed the stabilization of the curcumin molecules within the LDH layers. Drug release characteristics of the nanohybrid was quantitatively monitored under different pH (3 & 5) conditions using UV-Visible absorption spectroscopy. Finally, the antimicrobial activity of turmeric-LDH nanohybrid was tested against three bacterial species including Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 25853) and two fungal species such as Candida albicans (ATCC 10231) and Candida dubliniensis (clinical isolate) using agar well diffusion method. The results show that the drug release of turmeric encapsulated LDH was a slow process, suggesting that the intercalated nanohybrid can be used as an efficient drug formulation.
Arjun Singh
Central Council for Research in Ayurvedic Sciences, India
Title: Comparative phytochemical study of heartwood versus small branches of acacia catechu using high performance thin layer chromatography
Biography:
Arjun Singh is working as Research Officer (Chemistry) in Central Council for research in Ayurvedic Sciences (CCRAS), Ministry of AYUSH, Govt. of India, New Delhi-58. He has 81/2 years experience in Research&Development, Standardization, Quality Control and Analysis of Drugs & Pharmaceuticals, Ayurvedic drugs & plant based medicines. He has also been worked (about 9 Years) in Central Revenue Control Laboratories, Dept. of Revenue (CBEC), Min. of Finance, Govt. of India and carry out Chemical analysis(using conventional & instrumental methods) of samples of various commodities for Revenue (Excise & Custom duty)purpose with a view to Export/Import / Excise Tariff act & policy. Presently he is engaged in various activities viz. Prepared SOPs and protocol for Standardization of Herbal drug and pharmaceutical, Pharmaceutical development of AYUSH CODED DRUGS and double blind randomized coding for clinical trial, Preparation of know how documents of drugs for patent filing, Technology transfer & commercialization. Prepare Drug Dossier & Drug Profile of Ayurvedic Drugs for preclinical/ clinical studies, Evaluation of EMR & IMR projects etc. He has published more than 20 papers, 7 Books / Mongraph(contributor), 3 Patent filed and 4 projects completed and also serving as an editorial board member of JDRAS.
Abstract:
To compare the phyto-constituents present in the heartwood and small branches of Acacia catechu on the basis of high performance thin layer chromatography in order to evaluate whether the small branches of this plant may be substituted with the heartwood. Acacia catechu (Family- Mimosaceae) commonly called Khadira is a medicinal plant widely used in Ayurveda. As per the Ayurvedic literature, heartwood of this plant is used in sáotha, Kustha, Prameha, Vrana. It is difficult to get huge amount of heartwood from this big tree without cutting the plant. Due to which availability of this plant may be difficult in near future for use in Indian system of medicine. Methods: CAMAG HPTLC system equipped with semi-automatic applicator Linomat-IV and win CATS 1.4.2 software was used. n-hexane, ethyl acetate and ethanolic extracts of the heartwood and small braches were developed in suitable mobile phase using standard procedures and visualized in UV 254 and 366 nm and in white light after derivatization with in Anisaldehyde-Sulphuric acid reagent. Results: The HPTLC fingerprinting of the n-hexane, ethyl acetate and ethanolic extracts of heartwood and small branches showed almost phytochemical profile. Conclusion: HPTLC fingerprint profile of heartwood and small branches are found similar, therefore small branches may be used in place of heartwood and vice-versa after comparison and confirmation of same for pharmacological activities. The results of qualitative evaluation of HPTLC fingerprint profile will also be helpful in the identification and quality control of the drug and can provide standard HPTLC fingerprints with selected solvent system. The method can also be used for identification of different Acacia catechu species and adulterants.
Ciddi Veeresham
Kakatiya Univeristy, India
Title: Development and Evaluation of selected Indian medicinal plant based poly herbal Anti-Aging cream
Biography:
Will be uploaded soon.
Abstract:
Practice of using herbal remedies as a strategy against aging skin has been evolved as an imperative step to accomplish comprehensive skin rejuvenation. Present study is aimed to prepare and evaluate a poly herbal cream containing standardized extracts of Aloe vera (A), Ocimum sanctum (O) and Punica granatum peel (P) at different ratios. Santalum album, Citrus aurantium, Olea europa, Prunus amygdalus oils, Cetyl alcohol and Stearic acid were used in a ratio of 0.1:0.1:1.0:0.5:0.5 and 3.0 respectively, along with preservatives to formulate a cream base for formulation water in oil (W/O) creams i.e. F1 to F6. Prepared formulations were evaluated for formulation parameters like pH, viscosity, spreadabilty, extrudability and stability. Further, formulations were also evaluated for anti-oxidant, anti-microbial and advanced glycation end product inhibition (AGE) properties. Formulations F3-F6 have shown no phase separation upon storage. Formualtions F3 and F6 have featured lower spreadability and extrudabiltiy. The order of anti-oxidant ability of the all formulations found as F6>F5>F4>F3>F2>F1. Formulations F4 and F5 shown comparable AGE inhibitory and anti-microbial effects to standards Aminoguanidine and Neomycin sulphate cream, respectively, however F4 shown higher AGE inhibitory effect vis-a-vis with F5. In human volunteer’s study, the formulations F4 and F5 shows no redness, edema, inflammation and irritation during irritancy studies. The synergistic effects of formulation F4 containing A: O: P (each 1.6% w/w) has addressed hyperpigmentation, erythema, wrinkles and firmness of skin more significantly than F5. These finding indicate the significant anti-aging potential of examined poly herbal anti aging cream.
Bhushan P Pimple
P. E. Society’s Modern College of Pharmacy, India
Title: Concomitant Management of Type-II Diabetes and its Associated Hyperlipidemia: Role of ethyl 2-[5-(acetyloxy)pently] benzoate
Biography:
Bhushan P. Pimple has completed his PG from University of Pune. He is working as Head, Department of Pharmacognosy, in PES Modern College of Pharmacy, Nigdi, Pune 411044 from last 10 years. He has published more than 6 papers in reputed international journals and has been serving as an reviewer to six international journals
Abstract:
Diabetes mellitus is a metabolic disorder prevailing throughout the world irrespective of age, sex and race. Moreover, complications associated with chronic diabetic conditions are severe and fatal. Normally, the patient exhibits cardiovascular disturbances, nephrological and neurological disorders. Objective: The present study focuses on management of type-II diabetes and associated hyperlipidemia with the use of ethyl 2-[5-(acetyloxy)pently] benzoate isolated from O. majorana leaves. Method: Streptozotocin, STZ (65 mg/Kg, i.p.) along with nicotinamide (120 mg/kg, i.p.) was used to induce type- II diabetes mellitus (NIDDM) in rats. Lipid profile was estimated after every 7 days for a period of 28 days. The rats were treated with various doses of ethyl 2-[5-(acetyloxy)pently] benzoate. The antidiabetic effect was compared with pioglitazone and antihyperlipidemic effect with Mevastatin. Results: Ethyl 2-[5-(acetyloxy)pently] benzoate significantly lowered the FBG in vivo and also elevated glucose absorption in isolated rat hemidiphragms. Our studies also revealed that the antihyperlipidemic property of ethyl 2-[5-(acetyloxy)pently] benzoate was comparable to Mevastatin. Furthermore, software assisted docking also proved the effective binding of ethyl 2-[5-(acetyloxy)pently] benzoate with PPAR-γ and HMG-coA reductase. Conclusion: Ethyl 2-[5-(acetyloxy)pently] benzoate can serve as a better candidate in the concomitant management of diabetes and its associated complications such as hyperlipidemia due to its versaltile nature.
- Young Research Forum
Location: Hyderabad International Convention Centre, India
Session Introduction
S Gomathi
J K K Nattraja College of Pharmacy, India
Title: Phytochemical characterization and neuroprotective assessment of standardized extract of Pedalium murex Linn. leaves in endotoxemia-induced neurodegenerative model in rats
Time : 16:10-16:25
Biography:
Will be uploaded soon
Abstract:
An attempt has been made to understand the protective role of ethanol extract of Pedalium murex Linn. herbs (EEPM), by employing LPS to induce neurodegeneration. Animals were divided into six groups. The animals were exposed to LPS (xxmg/kg; i.p.) once (on day 1) and the changes in food and water intake, body weight and general behaviour were observed for 30 days and the effect of EEPM on the perturbations in antioxidant defence elements in hippocampal region of rat brain were also studied. Further, histopathological (hippocampal CA1) tests were carried out to substantiate the study. In addition, isolation, characterization and standardization of the chosen molecule were also carried out and the molecule was further subjected to in vitro antioxidant studies. Neurodegenerative diseases are one of the most debilitating, with respect to medical, financial and associated social issues. Currently, effective drugs for treating neurodegenerative diseases have led to discovery of new molecules from nature. Recent advances in the basic knowledge of such diseases have led to a re-evaluation involving new therapeutic approaches. Like other herbs, the neuroprotective effect of Pedalium murex in endotoxemia-induced neurodegenerative model is debatable and its neuropharmacological profile has not been investigated so far. This study may shed light on the potential role of Pedalium murex in such diseases and its development/discovery may herald a new therapeutic agent from nature.
Santosh Kumar Rath
CSIR-Indian Institute of Integrative Medicine, India
Title: Chemical investigation of bioactive secondary metabolites from Allamanda cathartica
Time : 16:25-16:40
Biography:
Santosh Kumar Rath is pursuing his Ph.D from CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu, (India) under the supervision of Dr. P. L. Sangwan, Scientist, BOC Division. He is registered for Ph.D in Chemical Sciences in AcSIR session Aug. 2013. He has one published paper in reputed journals. He is working on natural product isolation from plant origin and performs structural modification of major bioactive isolates.
Abstract:
Allamanda cathartica belongs to Apocynaceae family which is native to Brazil, also found in India and Srilanka that has an important medicinal value. In India it is mostly distributed in the eastern costal region of Odisha locally known as ‘Malatilata’, ‘Ghanta Phul’, ‘Harkakra’. The plant is traditionally used in Ayurvedic and Unani system of medicine for the treatment of various ailments such as healing of wounds and ulcers. The bark acts as a hydragogue in ascites while the leaves are a valuable cathartic in moderate doses. The root is used as a remedy for snake-bites. The whole plant is used against jaundice, malaria, fungal infections and antiseptic for the treatment of skin burns. Alcoholic and aqueous extracts of root possess hypotensive activity while leaf extract showed antifungal and anticancer properties. Considering its importance in Indian System of Medicine (ISM) and traditional use, the chemical investigation of whole plant was carried out which resulted in the isolation of eleven medicinally noteworthy secondary metabolites like allamandin (1), allamdin glucoside (2), betulinic acid (3), ursolic acid (4), 2,6,8-trihydroxy-3-(4-hydroxyphenyl)-4H-chromen-4-one (5), pinitol (6), heptacosanoic acid (7), heptacosane (8), β-sitosterol (9), β-sitosterol-3-O-glucoside (10), and sucrose (11). Betulinic acid, pinitol and flavonoid are reported first time from this plant. The structures of isolated compounds were elucidated mainly by one-dimensional, two-dimensional NMR, MS studies and by comparison with the spectral data in literature. Ursolic acid (4) was found major component (natural abundance: 1.118%), allamdin glucoside (2) (0.0353%) and betulinic acid (3) (0.019%) the least which is advantageous as all are known for potent anticancer, anti-microbial activities.
Tao Su
Hong Kong Baptist University, China
Title: Comparison of the toxicities of raw and processed Pinelliae rhizoma in rats
Time : 16:40-16:55
Biography:
Tao Su has completed her MPhil from Nanjing University of Chinese Medicine. Now, she is a PhD candidate of Hong Kong Baptist University. She has published more than ten papers in reputed journals. Her relevant research work is Chinese Pharmacology.
Abstract:
Pinelliae rhizoma (Banxia) is the dried tuber of Pinellia ternata. It has received great attention because of its excellent therapeutic effects as well as the associated toxicity. Banxia can cause irritant toxicity to oral, throat and gastrointestinal mucosa, as well as cardiotoxicity. To reduce the toxicity, Banxia is usually processed with ginger juice and alumen. The processed product is called Jiang-Banxia. Here, we used the blood biochemistry, histo-pathological examinations and metabonomics methods to compare the toxicities of Banxia and Jiang-Banxia in rats. Male SD rats were divided into three groups. Treatment groups received oral administration of Banxia or Jiang-Banxia powder suspended in 0.5% CMC-Na at a dose of 3 g/kg/day for 14 days, the control group received equal volume of the vehicle. Results showed that the Banxia-treated rats had smaller body weight gain and less physical activity compared to rats of control group, as well as obvious body trembling, aphony and diarrhea. Compared with the Banxia group, rats in Jiang-Banxia group showed better symptoms. The biochemical and histo-pathological results suggested that Banxia possessed toxicity, and Jiang-Banxia was less toxic. Differences of metabolic profiles involving serotonin and proline metabolisms were observed among vehicle-, Banxia- and Jiang-Banxia-treated rats, suggesting serotonin and proline metabolisms were involved in the toxicity of Banxia. Our data verified that processing with ginger juice and alumen can reduce the toxicity of Banxia, and shed new light on the pathological mechanism of Banxia.
Syed Kasfur Rahman
MS Ramaiah Medical College, India
Title: Nature’s retort to the dengue crisis
Time : 16:55-17:10
Biography:
Will be uploaded soon
Abstract:
Dengue, an arthropod borne viral disease, has recently been considered as a global threat due to its dramatic resurgence and involvement of vast geographical areas, infecting about 2.5 billion people every year.Owing to its four closely related, but antigenically distinct serotypes, an adequate drug or vaccine against dengue virus is not yet available. So far innumerable fruitless approaches have been tried for screening of compounds against this virus, such as structure-based computational discovery and targeting of the viral enzymes. Due to an increasing trend in complementary and alternative medicine in the last few decades, researchers have come up with natural compounds which can be considered safer as compared to the synthetic drugs available for this condition. Several medicinal plant extracts such as Psidium guava, Carica papaya, Euphorbia hirta etc. have been found to exhibit anti dengue activity through their wide spectrum of mechanisms of action such as, inhibition of mRNA of the virus, stimulation of genes that code for platelet production, membrane-stabilizing property and protection of blood cells against stress-induced destruction. This review highlights the manifold natural options available at present to alleviate the symptoms & hasten the recovery in these patients, when given as an adjunct to the standard regimens
Shubham Ukarande
Govt.college of Pharmacy,Aurangabad, India
Title: Phytochemical and Pharmacological studies of Trichodesma Indicum,(family:Boraginaceae)
Time : 17:10-17:25
Biography:
Shubham Ukarande is persuing B.pharmacy last year from Govt. College of pharmacy,A’bad.
Abstract:
Trichodesma indica (Boraginaceae) aerial parts is commonly used in the traditional systems of medicine against various ailments like urinary tract disorders, skin irritations, worm disturbances, as a component in health tonic preparation etc. The present study gives first insight of anti-microbial, anthelmintic, anti-oxidant properties and preliminary phytochemical analysis of the aerial parts of Trichodesma indica. All the analysis was done according to standard protocols.The ethanolic extract produced significant anti-bacterial, anti-fungal and anthelmintic properties in a dose-dependent manner. Preliminary phytochemical screening revealed the presence of alkaloids, steroids, flavonoids, tannins and phenols that may be the reason for its biological properties. This paper reporting the medicinal property of Trichodesma indica aerial parts and the further procedures of identification and isolation of active principles are in progress.
Rohit Gupta
Dadasaheb Balpande College of Pharmacy, India
Title: Effect of piperine on inhibition of FFA induced TLR4 mediated inflammation and amelioration of acetic acid induced ulcerative colitis in mice
Time : 17:25-17:40
Biography:
Rohit A. Gupta has completed his M. Pharm at the age of 24 years from Rashtrasanth Tukdoji Maharaj Nagpur University Nagpur from Institute of Pharmaceutical Education and Research, Wardha. He is working as Asst. professor at Dadasaheb Balpande College Of Pharmacy, Besa, Nagpur and had recently registered for his Phd at UDPS, Nagpur. He had published 2-books, 3-international papers and attended several national/international conferences. He is also serving as Director for Pharma Tutorials Pvt. Ltd, Nagpur a premier institute providing education for various entrance exams in Pharmaceutical science.
Abstract:
Ethnopharmacological relevance: Piperine, a main component of Piper longum Linn. and Piper nigrum Linn., is a plant alkaloid with a long history of medicinal use. Piperine exhibits antidepressant, hepatoprotective, anti-metastatic, anti thyroid, immunomodulatory, antitumor and anti-inflammatory activities; however its therapeutic potential in amelioration of ulcerative colitis and the underlying mechanism for anti-inflammatory activity remains unknown. Aim: The objective of the present investigation was to unravel the therapeutic potential of piperine on amelioration of IBD using acetic acid induced experimental animal model for ulcerative colitis and to determine the role of TLR4 receptor in signalling pathway of inflammatory gene expression in ulcerative colitis. Materials and Methods: We induced colitis using acetic acid (150µl of 5% once, intrarectally) in mice and estimated disease activity index (DAI), which took into account weight loss, stool consistency, occult/gross bleeding. Colon length, spleen weights, ulcer area and ulcer index were measured, Histological changes were observed by H&E staining. Effect of piperine on various antioxidant parameter of mice colon such as tissue myeloperoxidase (MPO) accumulation, SOD concentrations, reduced GSH and Lipid peroxidation were determined. Pro-inflammatory mediators, namely, nitric oxide (NO), tumor necrosis factor-α (TNF-α) were determined by TNF-α ELISA kit obtained from Thermo fisher scientific India pvt.ltd. Effect of piperine on haematological parameters of mice in acetic acid induced IBD was also determined which involves the estimation of FFA using commercial free fatty acid fluorometric assay kit. Result: Piperine significantly attenuated acetic acid induced DAI score which implies that it suppresses weight loss, diarrhoea, gross bleeding and infiltration of immune cells. Piperine administration also effectively and dose dependently prevented shortening of colon length and enlargement of spleen size. Histological examination indicated that piperine reduces edema in sub mucosa, cellular infiltration, reduced haemorrhages and ulceration as compare to acetic acid induced colitis in mice. Furthermore piperine inhibited abnormal secretion of pro-inflammatory mediators namely NO, cytokines TNF-α and reduces FFA induced TLR4 mediated inflammation. Conclusion: These results suggest that piperine has an anti-inflammatory effect at colorectal sites that is due to down- regulations of the productions and expression of inflammatory mediators and it also reduces FFA induced TLR4 mediated inflammation. Thus it may have therapeutic potential on amelioration of IBD. Keywords: Piperine, TNF-α, Ulcerative colitis, Free fatty acid (FFA), Toll like receptor (TLR4)
shyamala chennaboina
Kakatiya university, India
Title: Hepatoprotective activity of Murraya koenigii leaf extract on paracetamol induced hepatic damaged rats.
Time : 17:55-18:10
Biography:
Will be uploaded soon.
Abstract:
In the present study protective effect of Murraya koenigii leaf extract was investigated against paracetamol induced hepatotoxicity and compared with silymarin a standard hepatoprotective reference drug. Methanolic extract was prepared by uing soxhlet method. Male Albino wistar rats were divided into 5 groups. Rats except of control group received a single dose of paracetamol (2gm/kg.b.wt) on 7th day,silymarin (100mg/kg.b.wt) and extract (200 and 400mg/kg.b.wt ) for 7 days. On the 7th day blood samples were collected by retro orbital puncture and liver marker enzymes (sgot,sgpt,alkaline phosphatase),total protein,total bilirubin levels were estimated in serum and histopathologicl observations were done for the isolated livers. In the toxic group paracetamol treatment lead to elevated level of liver marker enzymes and bilirubin and there was deterioration of total protein content. However treatment with Murraya koenigii shows a significant results (p<0.05) in comparision with standard drug. Thus the results clearly demonstrate that murraya koenigii possess promising hepatoprotective activity,hence suggests its use as potential therapeutic agentfor protection from paracetamol overdose.
Biography:
Divya has completed her M.B.B.S from NTRUHS and currently pursuing M.D Pharmacology at S.S Institute Of Medical Sciences and Research Centre. She has undertaken a study on ‘ prescription pattern of drugs in Upper respiratory tract infections’
Abstract:
Ethnopharmacology is the scientific study correlating ethnic groups, their health, and how it relates physical habits and methodology in creating and using medicines. It can be defined as a multidisciplinary study of biologically active agents used in traditional medicine. As there are many disciplines that contribute to this branch, the research methodologies are diverse. Plants and plant based remedies constitute a complex mixture of compounds and identifying the active principles that are relevant for the pharmacological properties is not an easy task. Nevertheless the interest in identifying these compounds with therapeutic effects is on a rise, as they form the lead compounds for developing new drugs. The loss of language and traditional knowledge due to acculturation and plant habit destruction is a major concern especially among smaller tribes. Loss of indigenous knowledge has an impact on modern medicine. There is an imminent need of resurgence of ethnopharmacology as the knowledge of the use of plants is disappearing faster. Researchers in many disciplines including pharmacology, must accelerate the studies of ethnomedicine, for developing new drugs from natural products needed by humans.
Koushik Nandan Dutta
Assam Down Town University, India
Title: Effect of Mucuna pruriens (Linn) on Acute dermal toxicity, Electrocardiography & Respiratory variable of Wister rat
Biography:
Koushik Nandan Dutta has completed his M.Pharm in 2013 from Uttarakhand technical university,Dehradun and enrolled PhD in Assam Down Town University, Guwahati, Assam.He has completed 1 year project at Defence Research laboratory, Tezpur Assam. Currently he is working as a Assistant Professor in the department of pharmaceutical science in Assam Down Town University. He has published eight publications in reputed journals.
Abstract:
Mucuna pruriens(Linn) is Known as velvet bean or cowitch found in Africa, India and the Caribbean belonging to the family Fabaceae. Mucuna pruriens Linn is a herbaceous vine,generally tap rooted and reproducing by seeds,leaves have three leaflets up to 15 cm long,densely hairy beneath and rather silvery the lateral leaflets asymmetrical. Hair lining of mucuna seeds were tested for acute dermal toxicity. Hair lining were mixed with dusting powder and prepared three formulations according to concentration. This test was performed on rats based on OECD guideline number 404 (OECD, 1981a). In case of acute dermal toxicity study there was no treatment related mortality in any of the groups following topical application of formulations in either male and female rats for 14 days .Hematological Studies revealed that after application of tested formulation WBC like monocyte, neutrophil, eosinophil were increased. Serum biochemistry data did not produce significant differences between control and treated groups of both sexes in the majority of cases.In case of electrocardiography the animals showed normal value as compared to the control group. In whole body plethysmograph the treated animals showed similar value as compared to the normal group . After Sacrifice, the gross examination of the vital organs like liver, kidney and lungs, no noticeable hemorrhage and abnormal conditions were observed. The histopathological study did not showed any type of abnormality of skin in all treated group. It can be concluded from the finding of the present study that the findings of these studies support the safety use of the test substance Mucuna pruriens.
Biography:
Purbajit Chetia has completed his M.Pharm in 2012 from Sikkim University and enrolled PhD in Assam Down Town University, Guwahati, Assam. Currently he is working as a Assistant Professor in the department of pharmaceutical science in Assam Down Town University. He is having a few research publication in reputed journals. His area of interest in Rheumatoid Arthritis.
Abstract:
Rheumatoid arthritis (RA) is a chronic, autoimmune and systemic inflammatory disorder that mainly attacks synovial joints resulting in an unchecked synovial inflammation that leads to increased oxidative stress in peripheral blood inflammatory cells. RA typically affects the joints of hands and feet, although any joint in the body may be affected such as wrists, knees, hips and shoulders resulting in permanent damage, bone destruction and deformity. The precise etiology of RA remains still unknown, but studies have implicated a role for oxidative stress and redox signaling in its pathogenesis. Lymphocytes are considered to play a key role in the pathogenesis of inflammatory diseases like RA. The disease is consistently associated with an increase in various pro-inflammatory factors that include cytokines (IL-1 β, IL-6, tumor necrosis factor alpha TNF- α), prostaglandins and also reactive oxygen and nitrogen species (ROS/RNS) at sites of inflammation that leads to damaging of cellular elements in cartilage and components of the extracellular matrix like protein and lipid. This present study was aimed to evaluate the ex vivo effect of plant derived biomolecules (β-carotene and Naringenin, which are already proved to have free radical scavenging property) on generation of free radicals in isolated blood lymphocytes of patients with RA. Interestingly it was found that these biomolecules significantly inhibited the superoxide, hydroxyl and NO radicals attenuating oxidative stress in the isolated peripheral blood mononuclear lymphocytes of patients with RA that admire further studies on other human inflammatory cells.
Prasanthi doppalapudi
Sri Padmavathi Mahila Visvavidyalayam, India
Title: Pharmacognostic and physicochemical analysis of the seeds of citrullus lanatus l
Time : 18:10-18:25
Biography:
Prasanthi doppalapudi completed her B-Pharmacy and M-Pharmacy from Dr. H. L.T. College of Pharmacy, Kengal, Channapatna, Bangalore rural district, Karnataka. I am pursuing my Ph.D. as a fulltime research scholar under the guidance of Prof. A. Sreedevi in the Institute of Pharmaceutical Technology, Sri Padmavati Mahila Visvavidyalayam, Tirupati, Andhra Pradesh. I am a Principal Investigator of DST-WOS-A Project. Three articles were published in reputed journals and attended 2 international and 14 national conferences.
Abstract:
The main objective of the present study was to evaluate pharmacognostic properties including macroscopic, microscopic and physicochemical characters of the seeds of Citrullus lanatus. Citrullus lanatus seeds used for this study were purchased from Srisu Agrichem Private Ltd., Kalyanpuri, Uppal and authenticated by Botanist Dr. Madhava chetty, Herbarium keeper, Department of botany, Sri Venkateswara University, Tirupati, India and a specimen (Specimen No:640) has been deposited in Department of Botany, Sri Venkateswara University, Tirupati, India. Seeds were allowed for shade drying (except for microscopic studies) and seeds were subjected for pharmacognostic study including microscopic and macroscopic characters of the seed and seed powder, physico-chemical parameters such as total ash value, acid insoluble ash, water soluble ash, alcohol soluble extractive, water soluble extractive value, pH, moisture content, foreign matter present, foaming index, swelling index, etc. Above mentioned parameters were carried out by following standard methods. Fluorescence analysis of the seeds was conducted. Preliminary phytochemical studies were also conducted. Microscopic studies revealed the presence of thick walled trichomatous epidermis with mucilaginous hairs, sclerenchymatous hypodermis and wide circular parenchymatous inner seed coat. Physicochemical parameters had shown characteristic results such as total ash value of 3.5%, and pH ranging from 6.5-6.55 for 1% solution and 5.90 to 5.95 for 10% solution. Preliminary phytochemical studies revealed the presence of carbohydrates, tannins, glycosides, steroids, saponins, flavonoids and oils and fats. The present study i.e., pharmacognostic studies of Citrullus lanatus is helpful in the characterization of the crude drug.