Pramod Hurkadale
K L E University, India
Title: In vitro studies on Achyranthes coynei Santapau. for micropropagation
Biography
Biography: Pramod Hurkadale
Abstract
Achyranthes coynei Santapau., a rare, endemic medicinal plant was successfully propagated in vitro using apical shoot tips as explants. A combination of 3.0 mg.L-1 of 6- Benzylaminopurine (BAP), 0.5 mg.L-1 Thidiazuron (TDZ) and 0.5 mg.L-1 Kinetin (KN) in Murashige and Skoog (MS) agar-solidified medium proved the best combination for multiple shoot induction, yielding 408 shoots per explants after 60 days of culture. The shoots were rooted with a frequency of 80% after 30 days of culture on MS agar medium fortified with IBA/NAA (0.5 mg.L-1) before being acclimatized in the green house.
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