Day 3 :
- Track 1: Advanced Trends for the Future of Herbal Drugs and Products
Track 3: Herbal Drugs and Formulations
Track 9: Natural Products of Medicinal Interest
Young Research Forum
Location: Hyderabad International Convention Centre, India
Session Introduction
Meenakshi Barua
Birla College of Arts, Science and Commerce, India
Title: Physico-chemical, pharmacognostical evaluation and cytotoxicity study of cassia angustifolia (vahl) leaves
Time : 09:45-10:00
Biography:
Meenakshi Barua has completed her Graduation in Environmental Science from Delhi University (2007) and Mastered in Biotechnology from KIIT University, Bhubaneshwar (2010). During her post-graduation she completed her Dissertation in Environmental and Industrial Biotechnology Division at TERI, New Delhi (Jan – Jul 2010). She qualified GATE, (Biotechnology) in 2011. At present she is pursuing her PhD in Biotechnology from Birla College of Arts, Science and Commerce, Kalyan (University of Mumbai). She is a visiting faculty in the college in the subject of Biotechnology and presented papers (Oral and Posters) in national and international conferences.
Abstract:
Cassia angustifolia Vahl (Family – Caesalpiniaceae) commonly known as ‘Senna’ is one of the medicinally important, drought-resistant small perennial shrub. It is used as a febrifuge in splenic enlargements, anemia, typhoid, cholera and is an excellent blood purifier. It is also a rich source of polyphenols, glycosides, flavonoids, steroids and anthracene derivatives. Study of phytochemical and pharmacological features of senna leaves is of keen interest due to its huge medicinal properties. In the present study, leaves of Cassia angustifolia (Vahl) were used for its Physico-chemical and Pharmacognostical evaluation. Futher Cytotoxicity bio-lethality assay was performed on leaves using two different extracts (aqueous and hydro-alcohol) to check its toxicity. In Physico-chemical parameters, total moisture content (0.87 + 0.02%), total ash (10.2 + 0.05%), acid insoluble ash (0.85 + 0.002%), water soluble ash (9.8 + 0.04 %), sulphated ash (11.46 + 0.004%), alcohol soluble (5.84 + 0.72%) and water soluble extractive value (17.28 + 0.01%) were studied. Heavy metal (mercury, arsenic, lead, chromium and cadmium) analysis was found to be less than 0.01ppm i.e. in permissible limit. Zinc, an essential micronutrient for human being, was also found in permissible limit (14.72ppm) in the leaves. In phytochemical screening, xanthoproteins,carbohydrates, tannins, alkaloids showed maximum presence in acetone, methonal and aqueous extracts. Total alkaloids (119mg/g) and flavonoids(160mg/g) were determined using standard methods. In Cyto-toxicity bioassay, mortality rate was found higher in aqueous extract (67%) than in the extract of hydro-alcohol (20%). Analysis of all these parameters may significantly help in its use for the formulation of new pharmaceutical drug.
Khwaja Amtul Raouf Qazi
MRM College of Pharmacy, India
Title: Green factory kite as a natural substitute to heme
Time : 10:00-10:15
Biography:
Khwaja Amtul Raouf Qazi has completed her MPharmacy (Pharmacology) from Jawaharlal Nehru Technological University, Hyderabad, with an aggregate of First Class with Distinction.
Abstract:
Hemoglobin is a vital blood component. Heme in hemoglobin is present in hydrophobic pockets. It is structurally similar to chlorophyll with a difference in Mg-centered porphyrin ring in chlorophyll and Fe-centered porphyrin ring in hemoglobin. The high percentage of chlorophyll content in a natural form as a grass juice is preferred in clinical conditions involving hemoglobin deficiencies. Green vegetation (chlorophyll) are a factorial area to produce porphyrin ring. This porphyrin ring resembles “Kite” because of its structural porphyrin-head & phytol-tail. Chlorophyll obtained undergoes a catabolic process and could have physiological roles. The Fe-Centered porphyrin ring obtained will act as a natural substitute in hemoglobin deficiency conditions. Iron remains in the ferrous (Fe++) state in both oxygenated and deoxygenated hemoglobin. While in met-Hb, ferritin, transferin iron is in ferric (Fe+++) form. The oxygenation of one heme molecule enhances and accelerates oxygenation of other heme molecules. Similarly, the release of one oxygen molecule promotes the release of other which is required to meet metabolic demand of blood. 3.34 mg of iron-centered porphyrin ring of chlorophyll may require maximally bind of 1.34 ml of oxygen to form 1 gm of hemoglobin. Thus, the main aim is to provide beneficial effect of Fe-centered porphyrin ring of chlorophyll as a natural substitute to heme. The result of study may help the hemoglobin deficient patients to meet their iron and health needs.
Behzad Mohaddesi
Saurashtra University, India
Title: Phytochemical evaluation and anti cancer screening of cardiospermum halicacabum Seeds extracts on different Human Cancer Cell Lines
Time : 10:15-10:30
Biography:
Behzad Mohaddesi has completed his M.Sc on Medicinal Plants Sciences from Gujarat Ayurved University, India. He is pursuing his Ph.D. degree on herbal drug technology in Department of Pharmaceutical Sciences, Saurashtar University, Faculty of Pharmacy. His area of work is on traditional medicine, ethno pharmacology and evaluation of biological activity of medicinal plants.
Abstract:
In modern life the growth of different acute and chronic disease increased and cancer is one of the disorders with significant prevalence rate among them. As knowledge has increased about the traditional medicine, the daily interest to use of natural products especially herbal drugs are becoming more from patients to physicians with aim to prevention of disease and safer way of treatment with lesser side effect for such conditions. Hence, this study undertaken to evaluate the effect of cardiospermum halicacabum a traditional medical plant. Cardiospermum halicacabum Linn. from Sapindaceae family, known as love in a puff in English and karṇasphoá¹a in Hindi. Different part of this plant such as leaves, fruits and seeds are traditionally used for treatment of various diseases in different system of medicine such as Ayurveda, Chinese, Homeopathy and Unani. The seeds of plant selected for this study and subjected to soxhlet extraction to obtain the various extracts, preliminary phytochemical studies performed for each extract and individually tested against four human cancer cell lines, HT-29, HCT-15, SK-MEL-2 and MCF7 with Sulforhodamine B colorimetric (SRB) cytotoxicity assay and compared with Doxorubicin a known anti cancer drug. The most active extract selected and subjected to phytochemical analysis by GC-MS to find out the probable active components. Result of this work indicated the potential medicinal value of cardiospermum halicacabum seeds as anti-neoplastic agents on human cancer cell and the GS-MS analysis showed the present of different fatty acids in the seed extract which can be responsible for the anti cancers activity.
Hridya Hemachandran
VIT University, India
Title: Evaluation of Pterocarpus santalinus Linn. F. methanolic extract as a natural melanogenesis inhibitor: In vitro study in B16F0 melanoma cell lines
Time : 10:30-10:45
Biography:
Will be uploaded soon
Abstract:
Melanin, the end product of melanogenesis determines the skin, hair and eye colour. Excessive production of melanin causes hyperpigmentation disorders which results in serious aesthetic problems. Pterocarpus santalinus also known as red sanders or raktha chandan is a red dye yielding tree commonly used in treatment of skin disorders and insect bites in Indian traditional medicine. Here, we investigate the anti-melanogenesis effect of methanolic extract of Pterocarpus santalinus by cell free tyrosinase assay, followed by cell viability, cellular tyrosinase and melanin content assay in B16F0 melanoma cell lines. The heart wood of Pterocarpus santalinus was extracted in methanol and the skin whitening ability was tested. It has been observed that methanolic extract of red sandal wood shows dose dependent inhibitory activity on cell free and cellular tyrosinase activity. No cytotoxic effect was observed in cells treated with the extract upto 200 µg/ml concentration. Decreased melanin pigmentation was also observed in cells treated with Pterocarpus santalinus methanolic extract. Our finding exhibit skin whitening ability of Pterocarpus santalinus methanolic extract and serves its use in treatment of several hyperpigmentation disorders and also as a skin whitening agent in cosmetic industry replacing synthetic toxic skin whitening agents.
Nabanita Chakraborty
ICAR- Central Inland Fisheries Research Institute, India
Title: Wet-land colonizers to anti-dandruff dermatitis: Study with swamp cabbage
Time : 10:45-11:00
Biography:
N Chakraborty is pursuing her PhD from Central Inland Fisheries Research Institute (CIFRI), Indian Council of Agricultural research under DST Women Scientist Fellowship (WOS-A). She published 7 papers in international journals and one book chapter.
Abstract:
The pharmaceutical evaluation of the aquatic macrophytes is an emerging aspect of allelochemistry. In this context, swamp cabbage (Ipomea aquatic Forssk; Convolvulaceae) a semiaquatic, tropical plant grown as a vegetable and a key ecological community in wetland ecosystem has been focussed. Our current study aims for microbial screening of Ipomea leaves against facultative anaerobic bacteria to catfish, Edwardsiella tarda and fungal dermatitis Malassezia globosa. Extraction of crude leaf extract followed by liquid-liquid extraction process and further crystallized and each fraction subjected to biochemical analysis viz. phenols, flavonoids and tannins followed by selective antioxidant and antimicrobial activity. The inductive inferences of the various assays showed the ethanol fraction (80%) of the plant to be a natural resource to antidandruff compounds against M. globosa and ethyl acetate fraction to be preventive against E.tarda. BLA has also been conducted to address preliminary cytotoxicity issues as water being a non-resource variable and the lethal concentration was found to be considerably safe. A statistical approach to dose response curve is attempted for prediction of desired doses and validating the results.
Snehal Bhavsar
North Maharshtra University, India
Title: Design, development and characterization of naringenin loaded solid lipid nanoparticles for oral delivery
Time : 11:15-11:30
Biography:
S K Bhavsar has completed MPharm (Pharmacognosy) from R C Patel Institute of Pharmaceutical Education and Research, Shirpur North Maharashtra University, Jalgaon. She was University Topper and Gold Medalist in MPharm and is now pursuing PhD from the same institute.
Abstract:
Naringenin (NAR) is highly lipophilic drug having log P value 2.5. The poor orally available NAR only 8% oral bioavailability was successfully encapsulated in compritol based Solid Lipid Nanoparticles (NAR-SLNs) for its ultimate use to target intestinal lymphatic transport system was prepared. Result showed that SLNs with mean particle size of 66.56 nm (polydispersity index, PDI=0.31) and surface electrical charge of approx. −12 mV, were produced by high pressure homogenization process. Response Surface Methodology (RSM) using the Central Composite Rotatable Design (CCRD) model was used to optimize formulations of NAR-SLNs. Particles were characterized using Differential Scanning Calorimetry (DSC), XRD and Scanning Electron Microscopy (SEM) to confirm their solid character and the homogeneous distribution of drug within the lipid matrix. In vitro release studies at pH 6.8 phosphate buffer (PBS) and % cumulative drug release of 94.97 at the end of 24 h. The accelerated stability studies showed that there was no significant change in the mean particle size and PDI after storage at 25±2 ËšC/60±5% RH for the period of three months. This study showed that the RSM–CCRD could efficiently be applied for the modeling of NAR-SLNs.
Xiu-Qiong Fu
Hong Kong Baptist University, China
Title: Involvement of TLR4/STAT3 signaling in the anti-melanoma effects of atractylenolide II
Time : 11:30-11:45
Biography:
Xiu-Qiong Fu has completed her MPhil at Southern Medical University in Guangzhou, China. Now, she is a PhD candidate of Hong Kong Baptist University. She has published more than ten papers in reputed journals. Her relevant research work is pharmacology of Chinese medicines.
Abstract:
Melanoma incidence and mortality rates are rapidly increasing. STAT3 (Signal Transducer and Activator of Transcription 3) and TLR4 (Toll-Like Receptor 4) have been shown to be activated in melanoma cells. Suppressing TLR4 or STAT3 signaling in tumor cells represents an antimelanoma strategy. Rhizoma Atractylodis Macrocephalae (Baizhu in Chinese), a Qi-tonifying Chinese medicinal herb, is commonly prescribed by traditional Chinese medicine doctors for treating melanoma. We have demonstrated that atractylenolide II (AT-II), isolated from Baizhu, can significantly inhibit B16 tumor growth at non-toxic doses in mice, which is associated with a potent suppression of STAT3 signaling. We have also found that AT-II inhibits melanoma metastasis in mice and cell migration in A375 and B16-F10 cells. Molecular docking indicated that AT-II could bind to TLR4/MD-2 receptor complex. MPLAs, specific TLR4 agonists, significantly diminish AT-II-afforded suppression on STAT3 phosphorylation/activation and melanoma cell migration. Based on these data, we concluded that inhibiting the TLR4/STAT3 pathway is involved in the anti-metastasis effects of AT-II. This study advances our understanding of the anti-melanoma mechanisms of AT-II, and provides chemical and pharmacological justifications for the clinical application of Baizhu in melanoma treatment.
Sonali Mohanty
S.S Institute of Medical Sciences & Research Centre, India
Title: Evaluation of anticonvulsant activity of Turnera diffusa in Albino Mice.
Time : 11:45-12:00
Biography:
Sonali Mohanty, completed MBBS in 2011 from Bharati Vidyapeeth Deemed University, Pune.Currently pursuing Post Graduation in M.D. Pharmacology at SSIMS&RC, Davangere. KMC
Abstract:
Turnera diffusa commonly known as Damiana has a long history of use in traditional herbal medicine throughout the world. The medicinal part is its leaves, which are harvested during the flowering season. Damiana is known to act as an aphrodisiac, antianxiety, cough-suppressant, analgesic etc.The present study is done to evaluate the anticonvulsant activity of Turnera diffusa in albino mice.For the same purpose a total of 60 animals were divided into 10 groups of 6 each. Among which 5 groups were subjected to Maximal Electroshock Seizure(MES) model and other 5 groups to Pentylenetetrazole(PTZ) induced seizure model. Normal Saline was given as control. Sodium valproate 50mg/kg body weight per orally was used as standard. Aqueous extract of T. diffusa was used in three doses 50, 75, 100 mg/kg body weight per orally. In MES model there was a marked reduction in duration of all the parameters (tonic hind limb flexion, tonic hind limb extension, clonus and postictal depression) and was significant when compared with control(p<0.001) and was comparable with standard.In PTZ induced seizures prolongation of duration of onset of seizures and decrease in duration of seizures were observed. When compared to control, results were significant (p<0.001) and were comparable with standard .This study showed that T.diffusa has anticonvulsant activity.Further research is required to explore the utility of damiana for treatment of epilepsy.
Nayana Matada Revanasiddaiah
S.S Institute of Medical Sciences & Research Centre, India
Title: Evaluation of antidepressant activity of Turnera diffusa in albino mice
Time : 12:00-12:15
Biography:
Nayana M.R,completed MBBS in 2012 from RGUHS. Now pursuing postgraduation in M.D. Pharmacology in SSIMS, RGUHS. KMC
Abstract:
Turnera diffusa commonly known as Damiana is used for its medicinal property. Medicinal plant is its leaves which are harvested during flowering season. In traditional medicine it is used as aphrodisiac, antidepressant, anti-anxiety, gastro-protective etc. In present investigation, aqueous extract of Turnera diffusa was evaluated for its antidepressant activity in Albino mice. Turnera diffusa was subjected to antidepressant activity evaluation at various doses that is, 50, 75, 125 mg/kg p.o, Amitriptyline(20mg/kg) was used as standard. All the drugs were given 30 min before the test to mice. Rodent models of depression such as Forced swimming test(FST) and Tail suspension test(TST) were used for study. Turnera diffusa exhibited significant antidepressant activity at 50, 75 mg/kg body weight respectively with reference to control and as well as comparable with standard. At a dose of 125mg/kg it showed significant antidepressant activity compared to standard. In FST and TST there was a significant reduction in immobility period in animals treated with T.diffusa compared to control group (p<0.001) and immobility period was comparable with that of standard. The present study showed that T.diffusa has antidepressant activity and may be used as an alternative or add on therapy for treatment of depression.
Divya Reddy
JSS Medical College, India
Title: Study of the diuretic activity in aqueous extract of roots of Anacyclus Pyrethrum in albino wistar rats
Time : 12:15-12:30
Biography:
Divya Reddy K, completed my MBBS from Mahadevappa Rampure Medical College, Gulbarga. Presently I am doing my post-graduation course in Pharmacology at JSS medical college, Mysuru. Karnataka as .My dissertation is on herbal plant for its anticancer activity on lung cancer cell lines. (Invitro study).I presented a poster presentation at carcinogenesis 2015-Mumbai on EAC cell lines .I have 2 publications.
Abstract:
The aim of the study is to evaluate the diuretic activity of aqueous extract of roots of Anacyclus pyrethrum. Albino wistar rats weighing 200-250 gms of either sex were randomly selected from Central animal facility, JSS Medical College Mysuru. They were divided into 3 groups of 6 rats each (n=6).The animals were fasted overnight. Next day group 1which is control-received normal saline (25ml/kg.p.o), Group 2 which is standard-Furosemide 20mg/kg body weight. Group 3 which is test received 100mg/p.o Aqueous extract of roots of Anacyclus Pyrethrum. The experiment was carried out in a diuretic cage. The results, were analyzed by one Way Analysis Of Variance (ANOVA) .There was a significant diuretic activity compared to that of control. P value of less than 0.05 was considered significant.There was significant increase in urine volume by the aqueous extract of roots of Anacyclus Pyrethrum when compared to the control group.While the excretion of Urinary electrolyte concentration revealed a diluted urine in the test group signifying the diuretic activity of the extract. Aqueous extract of Anacylus pyrethrum have a significant diuretic activity in Albino wistar rats. Further tests are to isolate the active principles responsible for the activity suggested & also required to evaluate diuretic property in human subjects.
Raman Rajeshkumar
JSS College Of Pharmacy, India
Title: Alterations in mitochondrial apoptosis regulating gene expression in human cervical cancer cell line using a new compound isolated from the bark of Symplocos cochinchinensis (Lour.) S. Moore
Time : 12:30-12:45
Biography:
Raman Rajeshkumar has completed his M Pharm from JSS College of Pharmacy, Ooty and doing his PhD at JSS University, Mysore. At present he is working as a lecturer, Department of Pharmaceutical Biotechnology at JSS College of Pharmacy, Ooty since last 8 years. His field of research work is mainly in Free Radical Biology and Cancer Biology.
Abstract:
Mitochondria have been reported that cancer cells exhibited aerobic-glycolysis. Although this was originally construed as suggesting that the function of mitochondria was defective, we instantly realize that cancer cells are in an altered metabolic state. Little care has been compensated to the potential mutations that can affect mitochondrial function in cancer, outside of specific variations in genes. We were reporting that the new compound isolated from Symplocos cochinchinensis (Lour.) S. Moore had stimulated the expression of a few genes in mitochondria in human cervical cancer cell line. The Hela cells were treated with EA1 with their IC50 concentrations obtained from cytotoxicity. Various incubation duration, such as 0h, 24h, 48h and 72h were selected for the study. To elucidate the apoptotic pathways activated by EA1, mitochondrial transmembrane potential loss, Semi quantitative RT PCR and Western Blot analyses were carried out to measure the expression of death receptor as well as their apoptotic genes and bcl-2 family genes. Analysis of these genes revealed a substantial reduction in the expression of bcl-2 and cox-2 and increase in the expression of Bax and p53 was observed. Cells treated with EA1 was compared with untreated control cells. The ß - actin gene was used as an internal house keeping control for the study. In the present study, we noticed that the EA1 time dependently regulated the expression of Cox-2. Our works also demonstrate that the initiation of apoptosis was closely connected with reduction in mitochondrial transmembrane potential.
Pradeep B
JJM Medical college, India
Title: A review on the potential therapeutic profile of carica papaya leaf extract in the management of dengue fever
Time : 12:45-13:00
Biography:
Pradeep has completed his MBBS at the age of 25 years from JJM Medical College, Davangere, under Rajiv Gandhi University of Health Sciences, Bangalore and is currently doing post-graduation from the same academic institution. Currently he is actively involved in many research activities as his academic interest.
Abstract:
Dengue is an acute viral infection with potential fatal complications. Dengue virus (DV) belongs to the family Flaviviridae which has four serotypes (DV-1 to 4). One of the most disturbing aspects of the problem of dengue is that there are no effective antiviral agents to treat dengue complications. Treatment for dengue is mainly symptomatic. Some cases require platelet transfusions and fluid management. Papaya, Carica papaya known in Ayurveda as Erand-karkati, has many benefits linked to other parts of this plant, as its leaves. Various clinical and preclinical studies have demonstrated a positive effect of its leaf extract in dengue cases with thrombocytopenia. It induces increase in platelet count within 2 days of first dose which is very much evident at the end of 5th day. It prevents Dengue Fever worsening into Dengue Hemorrhagic Fever and thus promotes prompt recovery, avoiding hospitalization. It provides additional benefits in patients with Dengue fever which include increase in the WBC count, improvement of liver functions, and normalization of clotting profile. Few adverse events reported, related to GI disturbances like nausea, vomiting with commercially available preparations of Carica papaya leaf extract (Tab. Caripill) with no effects related to drugs tolerability and safety. To conclude, the Carica papaya leaf extract provides an overall benefit in dengue cases and there is more scope for its use in the coming days as there is obvious evidence of its efficacy and safety and because there are no specific treatment options available, except the symptomatic approach.
Shashikala G
JJM Medical college, India
Title: Evaluation of effect of alcoholic extract of Tinospora Cardifolia on learning and memory in Alprazolam induced amnesia in albino mice
Time : 13:30-13:45
Biography:
Shashikala.G has completed MBBS at the age of 22years from J.J.M.Medical College, Davngere, Rajiv Gandhi University of Health Sciences, Bangalore. Currently postgraduate in M.D. PHARMACOLOGY from the same academic institution and involved in various curricular activities.
Abstract:
Learning is defined as the acquisition of information and skills, and subsequent retention of that information is called memory. Learning and memory can be a psychological process as well as a change in synaptic neural connectivity. Memory is the most common cognitive ability lost with dementia. The study was undertaken to evaluate the learning & memory effect of Alcoholic extract of Tinospora cardifolia in alprazolam induced amnesic mice.The study was carried out on albino mice, divided into 4 groups of 6 animals each (3-4months of age, weight 25-30g). Amnesia was induced by administering Alprazolam (2mg/kg body wt for 14 days) in all 4 groups for 14days from 1st to 14th day.Group 1 would be given memory impairing dose of Alprazolam (2mg/kg/p.o) alone for 14days. Group 2 would be given Piracetam (400mg/kg p.o) from 8th to 15th day. Group 3 would be given Alcoholic extract of Tinospora cordifolia 140 mg/kg, p.o. from 1st to 15th day. Group 4 would be given Alcoholic extract of Tinospora cordifolia 280 mg/kg, p.o. from 1st to 15th day.The learning and memory of the animals was assessed by employing Elevated plus maze [transfer latency (TL)] & Step-down type passive avoidance model[step down latency (SDL)]. The measured parameters TL & SDL were compared with standard drug Piracetam. In both EPM & SDA model results were comparable with the standard. Tinospora cardifolia, Indian medicinal plant useful for treatment of various ailments like Diabetes,Rheumatoid arthritis,Gout,Viral hepatitis,Gonorrhoea,Syphilis and Anemia can also be a useful alternative for treating Dementia and associated diseases like Alzheimer’s disease.
Deepa Halemani
JJM Medical college, India
Title: Evaluation of antidepressant effect of methanol extract of Aegle Marmelos (Bael fruit tree) leaves in mice
Time : 13:45-14:00
Biography:
Deepa halemani is currently pursuing his 2nd year post graduate in Department of pharmacology at J.J.M Medical college, India
Abstract:
Depression is one of the most common mental disorders, characterised by sad mood, loss of interest and pleasure, worthlessness and suicidal thoughts. Currently Tricyclic antidepressants and SSRIs are most commonly employed drugs for the treatment of depression. Synthetic drugs available for treatment of depression have various adverse effects like sedation, dry mouth, postural hypotension and tachycardia etc. Drugs obtained from natural sources are known to cause fewer side effects compared to synthetic drugs despite of same ability to cure disease. Therefore this study is undertaken to evaluate the antidepressant property of methanol extract of Aegle marmelos (AM) (an ayurvedic medicinal tree, commonly known as the bael fruit tree) leaves in mice. Methanol extract of Aegle marmelos (100mg/kg, 200mg/kg and 400mg/kg), 2% of Gum acacia (control), Imipramine 20mg/kg (standard) are administered orally to randomly divided albino mice of either sex. Antidepressant activity is assessed by Tail suspension test (TST) and Actophotometer (locomotor activity) models. In TST and Actophotometer models ANOVA with post hoc test was used to assess antidepressant activity between groups. AM of all doses (100mg/kg, 200mg/kg, 400mg/kg) have shown statistically significant decrease in immobility time (p<0.05) compared to control and results are comparable with standard in TST. In Actophotometer model AM in all the doses have shown statistically significant increase in locomotor activity (p<0.05) compared to control and results are comparable with standard.
Pavitra R Y
JJM Medical college, India
Title: Evaluation of antidepressant activity of aqueous extract of the Prosopis cineraria in albino mice
Time : 14:00-14:15
Biography:
Pavitra graduated MBBS from BLDE medical college, Karnataka. Now pursing postgraduation in Pharmacology from JJM medical college, Karnataka. I have attended various state and national conferences and presented poster in IPSCON 2013 and published few articles.
Abstract:
Depression is the most prevalent mental disorder. Basic neuroscience offers the promise of improving our understanding of disease pathophysiology, identifying novel mechanisms that can be targeted by more effective pharmacotherapies and screening of herbal sources of drugs. The leaves of Prosopis cineraria are selected for evaluating its antidepressant due to its traditional use in the management of various CNS disorder. Aim of the study: To evaluate the antidepressant activity of aqueous extract of the Prosopis cineraria in albino mice. Materials and methods: Albino mice -24, Plastic cylinder, Drugs-Imipramine 15mg/kg, Aqueous extract of Prosopis Cineraria [AEPC] 100mg/kg and 200mg/kg. A total of 24 Albino mice will be divided into four groups of 6 animals each using forced swimming method. Group I served as control which received distilled water. Animals of group II received standard drug Imipramine at a dose of 15mg/kg. Group III received the AEPC orally at a dose of 100 mg/ kg. Group IV received the AEPC orally at a dose of 200 mg/ kg. Drugs were dissolved in distilled water and given orally one hour before the experiment. Results: Analysis is done using ANOVA and Post hoc analysis. Immobility time decreased with increase in the dose of aqueous extract of Prosopis cineraria [AEPC] 200mg/kg > 100mg/kg with significant p value and is comparable with standard drug imipramine 15mg/kg.
Biography:
Will be uploaded soon.
Abstract:
The plants belonging toBerberis species are extensively used in various Ayurvedic, homeopathic and ethno-medicines as raw material or as an ingredient. Presence of various major and minor secondary metabolites and their amount plays very crucial role in the standardization and quality control of herbal medicines. Therefore, more objective and definitive methods are necessary for knowing about the chemical variability amongst the genuine and commonly available species of the same plant.In the present study an attempt has been made to critically analyse the variability among the popular Berberis species on the basis of conventional single marker based approach and subsequently using the more advanced method of HPLC fingerprinting with principle component analysis and similarity analysis. The experiments were carried out to throw light on the variation in extractive value (%) and berberine content (%) with species, location, part of the plant and season and use of comprehensive HPLC DAD profile with PCA to access the variability among different species and effect of location, and stage of harvested plants (vegetative and reproductive). It was concluded thatPCA models constructed as per the locations and species and similarity analysis done on the basis of comprehensive HPLC fingerprinting data could be very useful for the herbal industries for predicting the origin of raw material, variability study for the authentication and standardization of raw material in terms of location and species.
Chaitanya Sravanthi Kota
JNTU University, India
Title: Priliminary phytochemical investigation and pharmacological screening of caseariaellipticabark for antidiabetic activity
Time : 14:30-14:45
Biography:
Chaitanya sravanthi kota is pursuing her Phd at Jawaharlal Nehru Technological University, in the work titled “Phytochemical Investigation and Pharmacological Screening Of Selected Plants For Antidiabetic Activity and Associated Complications.” She is Associate Professor and head of Department of Pharmacognosy at Vignan Institute of Pharmaceutical sciences, an Pharmacy educational institute affiliated to Jawaharlal Nehru Technological University. Presented poster in “5th International conference on Medicinal Plants and Herbal Products”, at Manipal in 2013, and 66 th Indian Pharmaceutical Congress. She has Published about 12 articles in International journals. Attended conferences both National and International on GLP, Quality Control of Ayurveda medicine, Agriculture biotechnology.
Abstract:
The metabolic disorder which has become more common in world population is Diabetes mellitus. Ample number of formulations of medicines for Diabetes are available but still this disorder requires a search for a drug with no or considerablely less side effects. This thought gave scope for complementary therapies. A study on bark of Casearia elliptica was conducted for evaluation of antidiabetic activity in STZ induced diabetic rats. Rats of body weight 180-200gms were administered with STZ dose 60 mg/kg body weight to induce diabetes. Five rats were placed in each group . Rats were grouped into Normal group (Group I) ,Diabetic control treatedwith STZ (Group II), Diabetic rats treated with Glibenclamide (Group III), Diabetic rats treated with Casearia elliptica bark extract(Group IV). Group III is administered with 10mg/kg of standard drug Glibenclamide .The Caseariaelliptica extract were administered with doses of 200mg/kg & 400mg/kg using CMC solution (5%) as the vehicle. Blood glucose in Group IV,III,II, I, on 14th day were 164.4±1.29, 98.8 ±1.15, 291±0.71,107±1.14 respectively . Observation shows that standard is one and half times morepotent than Group IV in hypoglycemic effect at a dose of 200mg/kg.. Body weights of Group IV , III , II,I and on 14th day were198.22±1.23, 187.2±0.96, 165.8±1.2,193.5 ±1.5, and with no toxic effects were observed in the Test Group (Group IV). Body weights and blood glucose levels were compared with Group II. Proteins,Carbohydrates;secondary metabolites like Alkaloids, Steroids ,Saponins and Flavonoids were present in Casearia elliptica which were detected by preliminary chemical tests
Uzma Azeem
Punjabi University, India
Title: Evaluation of nutraceutical composition of Phellinus gilvus (Schw.: Fr.) Ryv.
Time : 14:45-15:00
Biography:
Uzma Azeem doing PhD under the guidance of Dr. Gurpaul Singh Dhingra, Professor, Department of Botany, Punjabi University, Patiala and co-supervision of Dr. Richa Shri Associate Professor, Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala.
Abstract:
Mushrooms have long been appreciated for their flavor, medicinal and nutraceutical attributes. They have been receiving much attention as good neutraceutical and pharmaceutical source because they are rich in various bioactive compounds. The genus Phellinus (family: Hymenochaetaceae) has been receiving special concern because of its potent nutritive and medicinal properties. There are various species of the genus Phellinus that are known to be used as a cure against various ailments like diarrhoea, stomachaches, inflammation, arithritis, hepatoprotection, detoxification, combating allergy and diabetes since ages. In the present investigation, the powdered basidiocarp of the mushroom Phellinus gilvus has been studied for its various neutraceutical properties in terms of its mineral constituents and fatty acid composition. The mineral composition of Phellinus gilvus has been determined by wavelength dispersive X-ray fluorescence (WDXRF) technique. The presence of various minor and major mineral nutrients having immense role in boosting human health have been detected. The fatty acids which are important nutrition and physiology components have been analyzed by GC-MS technique. The mushroom has been found rich in various nutritive and medicinally important saturated and unsaturated fatty acids. The present study revealed that the mushroom Phellinus gilvus can be used in neutraceutical and herbal drug formulations.
Pallavi M Kamath
JJM Medical college, India
Title: Evaluation of anti-convulsant activity of ethanolic extract of Murraya koenigii leaves in Wistar rats
Time : 15:15-15:30
Biography:
Pallavi M Kamath has completed MBBS from AJ Institute of Medical Sciences, Mangalore and is currently pursuing Post Graduate course in MD Pharmacology at JJM Medical College Davangere, Karnataka.
Abstract:
Murraya koenigii, commonly known as curry leaf, is a tropical-subtropical tree of family Rutacea which is native to India. The leaves of Murrya koenigii is commonly used as seasoning for curries. It is also used traditionally for the treatment of epilepsy. The aim of the present study is to evaluate the anticonvulsant activity of ethanolic extract of Murraya koenigii in wistar rats using pentylenetetrazole (PTZ) induced seizure model and maximal electroshock seizure (MES) model. Thirty adult rats of both sexes weighing 150-200g were equally distributed into five groups of six each and subjected to seizures induced by PTZ and MES. The ethanolic extract of Murraya koenigii 500mg/kg, 250mg/kg, 125mg/kg were studied for its anticonvulsant activity. The standard being Sodium Valproate 200mg/kg and Control being suspending agent- 1% tween 80 . All drugs were given orally one hour before the artificial induction of seizure. The test drug showed statistically significant result compared to control in both PTZ and MES models.
Arun Suresh
JJM Medical college, India
Title: Medicinal plants with skeletal muscle relaxant activity: A review
Time : 15:30-15:45
Biography:
Arun Suresh has completed MBBS from AJ Institute of Medical Sciences, Mangalore and is currently pursuing Post Graduate course in MD Pharmacology at JJM Medical College Davangere, Karnataka.
Abstract:
Nature provides us an abundant supply of plants with medicinal properties that can pave the way to innumerable drug discoveries. Skeletal muscle relaxants are used to treat muscle spasm and spasticity. Muscle spasm is a sudden involuntary contraction of one or more muscle groups and is usually an acute condition associated with muscle strain (partial tear of a muscle) or sprain (partial or complete rupture of a ligament). Skeletal muscle relaxants consist of both antispasticity and antispasmodic agents. This is a broad categorization and prescribers usually do not differentiate between them. The antispasticity agents like baclofen, tizanidine, dantrolene aid in improving muscle hypertonicity and involuntary jerks. Antispasmodic agents, such as cyclobenzaprine, are primarily used to treat musculoskeletal conditions. The side effects of antispasmodic agents and antispasticity agents cause them to be used with caution. Therefore there is a need to discover a newer alternative for skeletal muscle relaxants that has a safer profile. Including the prototype skeletal muscle relaxant Tubocurarine, most of the modern day skeletal muscle relaxants are directly or indirectly of plant origin. Hence there should be systematic search within the herbal products in an attempt to find a plausible replacement. In this review article, the data was compiled from the latest, original relevant research articles and aims to enumerate the medicinal plants that possess skeletal muscle relaxant activity, the bioactive extract involved, dosage used, animal models employed for assessment, active phytochemicals contained in them and their possible mechanisms of action.
Vipul Suryavanshi
Bombay College of Pharmacy, India
Title: Resazurin microtitre assay (REMA) for antibacterial and antifungal activity of herbs of three antidiarrhoeal formulations: Bilagyl® and Berbenterone® tablets and Berbenterone® suspension
Time : 15:45-16:00
Biography:
Krishnapriya Mohanraj (corresponding author) has completed her PhD (Tech) in Pharmaceutical Chemistry from Institute of Chemical Technology (ICT), Mumbai in 1998. She is Head of Department of Pharmaceutical Analysis at Bombay College of Pharmacy. Her expertise includes Computer aided drug design, synthesis and pharmacological evaluation, bioactivity guided fractionation of herbal extracts, enzymatic resolution, chiral chromatography using achiral and chiral columns, impurity profiling, analytical method development and validation, bioanalysis and structural elucidation
Abstract:
Bilagyl®, Berbenterone® tablets and Berbenterone® suspension are Ayurvedic formulations used for antidiarrhoeal therapy. Herbs present in these formulations [Aegle marmelos (Bilwaphal), Berberis aristata (Daruharida), Punica granatum (Dadimtvak), Holarrhena antidysenterica (Kutajchal), Syzygium aromaticum (Lavang), Myristica fragrans (Jaiphal), Quercus infectoria (Maiphal), Cyperus rotundus (Nagarmotha), Aconitum heterophyllum (Ativisha), Embelia ribes (Vidang)] were subjected to various extraction procedures and the extracts were investigated by Resazurin microtitre assay (REMA) against Salmonella typhi, Escherichia coli, Staphylococcus aureus, Shigella flexneri and Candida albicans. All plants investigated showed anti-infective activity but only certain extracts of the herbs investigated were active. Some extracts showed only antibacterial activity while some extracts showed both antibacterial and antifungal activity. Hexane extract of Embelia ribes (Vidang) showed lowest MIC values against E. coli (0.190-0.381 μg/ml), S. flexneri (0.190-0.381 μg/ml), S. aureus (0.190-0.381 μg/ml) and S. typhi (3-6 μg/ml). Chloroform extract of Myristica fragrans (Jaiphal) and Quercus infectoria (Maiphal) showed lowest MIC value against C. albicans (24.4-48.8 μg/ml). Ciprofloxacin [MIC values: E. coli (0.01-0.02 μg/ml), S. flexneri (0.04-0.08 μg/ml), S. aureus (0.08-0.16 μg/ml) and S. typhi (0.04-0.07 μg/ml)] and fluconazole (MIC against C. albicans is 4.8-9.7 μg/ml) were used as standard antibacterial and antifungal agents, respectively.
Damayanthi Dalu
Jntu university, India
Title: Antidiabetic, antihyperlipidemic and antioxidant properties of roots of ventilago maderaspatana gaertn. On streptozotocin-induced diabetic rats.
Time : 16:00-16:15
Biography:
Will be uploaded soon
Abstract:
The present investigation was aimed to evaluate the traditional claim of Ventilago maderaspatana (V.maderaspatana) in diabetes. Furthermore antihyperlipidemic and antioxidant properties were evaluated since hyperlipidemia and free radical damage leads to diabetes. Hence the present study was aimed to evaluate antidiabetic, antihyperlipidemic and antioxidant activity. Antidiabetic activity was evaluated by oral glucose tolerance test and streptozotocin-induced model. Antihyperlipidemic activity was evaluated by estimating lipid levels. In addition Ventilago maderaspatana was also evaluated for antioxidant activity employing catalase, lipid peroxidase and glutathione reductase methods. By soxhlet extraction process alcoholic, hydroalcoholic, chloroform and petroleum ether extracts were obtained. All these extracts except petroleum ether were evaluated for toxicity unto 3000 mg kg-1. In oral glucose tolerance test chloroform extract did not produce significant glucose lowering effect. Alcoholic and hydroalcoholic extracts of Ventilago maderaspatana elicited significant glucose tolerance effect. Hence VMAE and VMHAE were screened further by streptozocin induced diabetic model. VMAE and VMHAE significantly lowered blood glucose, triglycerides, total cholesterol, LDL cholesterol, VLDL cholesterol, creatinine, urea and increased HDL cholesterol, serum insulin and liver glycogen levels when compared to standard drug glibenclamide (10 mg kg-1). V.maderaspatana also increased catalase levels and decreased lipid peroxidase and glutathione reductase. VMAE and VMHAE elicited significant dose-dependent antidiabetic, antihyperlipidemic and antioxidant activity. VMHAE at 500 mg kg-1 induced more significant antidiabetic activity than VMAE (500 mg kg-1). VMAE at 500 mg kg-1 elicited more antihyperlipidemic and antioxidant activity compared to VMHAE (500 mg kg-1).
Deepen Pardeshi
Bombay college of Pharmacy, India
Title: Study of Microbial bio-transformation of volatile oils.
Time : 16:15-16:30
Biography:
Will be uploaded soon.
Abstract:
Microbial Biotransformation is the chemical reaction performed by micro-organisms and catalyzed by the enzymes within the microbial cell. When a substance cannot be synthesized artificially or is difficult to synthesize, then in such cases, biotransformation processes are useful. Products of biotransformation have a number of important pharmaceutical applications such as anticancer agent, antimicrobial agents, flavoring agents, etc. The current study comprises of evaluation of biotransformation of volatile oil components brought about by the yeast S. cerevisiae. We studied the effect of yeast on volatile oils namely clove oil, orange peel oil, herbs containing volatile oils namely Ajowan seed powder, and selective components of volatile oils namely vanillin and menthol. The orange peel oil was extracted by using hydrodistillation method. Sabouraud dextrose broth was used as culture media for the process. The volatile oil substrates viz. clove oil, orange peel oil, were introduced into the culture medium in concentration range of 0.1% to 0.5% v/v and ajowan powder, vanillin, and menthol were used in range of 0.1% to 0.5% w/v. The reactions were carried out for 10 days at incubation temperature of 37 °C. The reactions were monitored by HPTLC pattern of the chloroform extract of aliquots taken at the interval of 48 hours. The mobile phase used was Hexane-Ethyl Acetate-Glacial Acetic Acid (6.5:3.5:0.5). Amongst the five samples observed the vanillin and orange peel oil showed additional spots in TLC. Whereas, clove oil and menthol did not show any change in TLC pattern; indicative of inhibition of the cell growth at concentration of 0.1% and above. This is an important finding for the point of view of flavoring properties of products of biotransformation. Keywords: biotransformation, volatile oils, S. cerevisiae, Sabouraud dextrose broth, HPTLC.
Pankaj Bharali
Rajiv Gandhi University, India
Title: Biochemical constituents of four ethnobotanically important legume plant species from Arunachal Pradesh, India
Time : 16:30-16:45
Biography:
Pankaj Bharali is a PhD research scholar in Department of Botany, Rajiv Gandhi University, Arunachal Pradesh, under DST-INSPIRE scheme-New Delhi, Government of India.
Abstract:
Mankind is dependent on herbalism or botanical medicines since time immemorial. Well recognized as a biodiversity hot spot, Arunachal Pradesh, the lofty mountainous state of India, is blessed with rich variety of floral and faunal heritage greatly influenced by its diverse geography, topography and varied climate pattern. Among more than 500 medicinally significant plants, Albizia lucidior(Steud.)Nielsen, Acacia pennata (L.)Willd, Albizia chinensis (Osbeck) Merr. and Gymnocladus assamicus Kanj. ex P.C. Kanjilalare locally used as soap, shampoo, to treat stomach disorders and as lice repellent by the Adi people of the state. In view of the very important ethnobotanical properties, this study was carried out with the objectives of the detection and quantification of preliminary phytochemical constituents, quantitative study of the secondary metabolites, quantitative study of both micro- and macronutrients and evaluation of antioxidant activities of these four plant species. Preliminary phytochemical investigation indicated the presence of considerable amount of saponin in all the four species validating their use as a good source of soap or detergent. Among these four species studied, the seed pod of G. assamicus was found to containing highest amount of saponins. All of the four plant extracts showed significant antioxidant activity in DPPH antioxidant assay.
Ogbonna John Dike N
University of Nigeria, Nigeria
Title: Use of homo lipid for delivery of anti-malarial drugs
Biography:
Ogbonna is a Fellowship Scholar of The World Academy of Science (TWAS) at SNJB’s Shriman Sureshadada Jain College of Pharmacy, Neminagar, Chandwad, 423101, Nashik, India. He completed his Masters in Pharmaceutics, University of Nigeria, Nsukka. He has published more than 30 papers in reputable journals (local and international) and has been serving as an editorial board member of repute. His research interest is in natural products (plants especially polymers, lipids etc) as pharmaceutical excipients.
Abstract:
Solid lipid microparticles (SLMs) which are recent drug delivery system employed in the delivery of drugs in addition of having advantages of lipid formulations are biocompatible, less toxic, long term stability etc. In this study, lipid was extracted from the goat fat using wet rendering method. The lipid matrix was prepared by fusion method and the drug loaded (chloroquine phosphate, halofantrine, and artemether/lumefantrine) SLMs formulated by hot homogenization. The in vivo activity of the SLMs on Plasmodium berghei infected mice was done using Peter’s four day suppressive protocol and the histological studies performed after the mice were sacrificed. The SLMs were stable. The SLMs containing arthemeter/lumefantrine had 87.01 % parasite clearance while the commercial formulation of arthemeter/lumefantine had 80.0 % parasite clearance; SLMs containing chloroquine phosphate had 87.10 % parasite clearance while the commercial formulation of chloroquine phosphate had 84.12 % parasite clearance; SLMs containing halofantrine had 72.96 % parasite clearance while the commercial formulation of halofantrine had 85.71 % parasite clearance.The group G which received no treatment had 14.89 % parasite clearance. The SLMs formulation had good in vivo activity compared to the commercial formulatio and were not harmful to the vital organs of the mice therefore SLMs can be an alternative means of formulating these antimalarials.
Samkeliso Takaidza
Vaal University of Technology, South Africa
Title: Biological activities of species in the genus Tulbaghia: a review
Biography:
Samkeliso Takaidza is currently doing her first year of PhD in Biotechnology and works as a Research Technician at Vaal University of Technology where she completed MTech in Biotechnology in 2012. She graduated in 2005 with a BSc in Microbiology from University of Limpopo, South Africa. Her first degree was a BSc in Science with Education at Bindura University of Science and Technology, Zimbabwe.
Abstract:
Since time immemorial, plants have been used by several communities to treat a large number of diseases. Numerous studies on the pharmacology of medicinal plants have been done. Medicinal plants constitute a potential source for the production of new medicines and may complement conventional antimicrobials and probably decrease health costs. Phytochemical compounds in plants are known to be biologically active aiding, for example, as antioxidants and antimicrobials. The overwhelming challenge of drug resistance has resulted in an increasing trend towards using medicinal plants to treat various diseases, especially in developing countries. Species of the genus Tulbaghia has been widely used in traditional medicine to treat various ailments such rheumatism, fits, fever, earache, tuberculosis etc. It is believed that the species possess several therapeutic properties. This paper evaluates some of the biological activities of the genus Tulbaghia. It is evident from current literature that T. violacea is the most promising species. The other species of Tulbaghia still require further studies to ascertain their medicinal potential.
Syed Kasfur Rahman
MS Ramaiah Medical College, Bangalore, India
Title: Nature’s retort to the dengue crisis
Time : 17:00-17:15
Biography:
Will be uploaded soon.
Abstract:
Dengue, an arthropod borne viral disease, has recently been considered as a global threat due to its dramatic resurgence and involvement of vast geographical areas, infecting about 2.5 billion people every year.Owing to its four closely related, but antigenically distinct serotypes, an adequate drug or vaccine against dengue virus is not yet available. So far innumerable fruitless approaches have been tried for screening of compounds against this virus, such as structure-based computational discovery and targeting of the viral enzymes. Due to an increasing trend in complementary and alternative medicine in the last few decades, researchers have come up with natural compounds which can be considered safer as compared to the synthetic drugs available for this condition. Several medicinal plant extracts such as Psidium guava, Carica papaya, Euphorbia hirta etc. have been found to exhibit anti dengue activity through their wide spectrum of mechanisms of action such as, inhibition of mRNA of the virus, stimulation of genes that code for platelet production, membrane-stabilizing property and protection of blood cells against stress-induced destruction. This review highlights the manifold natural options available at present to alleviate the symptoms & hasten the recovery in these patients, when given as an adjunct to the standard regimens.
Biography:
will be uploaded soon
Abstract:
Dietary and phytochemical intervention are emerging as an important modality for cancer chemoprevention. Polymeric black tea polyphenols (PBPs) are the most abundant polyphenols in black tea, but the anti-initiating properties of PBPs, are poorly elucidated. In the present study, the mechanism of PBPs-mediated anti-initiation was investigated in mouse skin employing benzo[a]pyrene ( B[a]P) as a carcinogen. Methods: Animal studies were conducted after approval from the Institutional Animal Ethics Committee as per the Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA, Government of India) guidelines (Protocol No- 13/2010 , dated-March 28, 2012). On the dorsal skin of swiss bare mice topical pre-treatments with acetone (vehicle, 100 µl) or P3/P5/Pm (200µg in 100µl acetone) was carried out on three consecutive days. On the third day 20 minutes after topical application of acetone /P3/P5/Pm, animals were topically treated with B(a)P (1 mg, 100 µl acetone) and 24 hrs after the last application animals were sacrificed, skin was removed and stored at -80°C. Expression of Phase-I enzymes (CYP1A1 and CYP1A2), and their transcriptional regulators AhR (Aryl hydrocarbon receptor), AhR nuclear translocator (ARNT) , XAP-2 and HSP90 were studied by western blotting. Phase -1 Enzyme activity was studied by spectroflourometry and mRNA levels were checked with RT-PCR. Phosphorylation of AhR and association of AHR:ARNT was evaluated by immunoprecipitaton as well as binding of AHR to XRE was checked by EMSA and DNA adduct formation by immunohistochemistry. Simultaneously PGE2 levels and DNA damage were studies by ELISA. Results: Pretreatment of mice with PBPs significantly decreased B(a)P-induced enzyme activity, mRNA levels and protein levels of cytochrome P450 1A1 and 1A2. Although alone PBPs did not alter the basal levels of AhR, it significantly diminished the B(a)P-induced AhR levels, nuclear translocation, subsequent binding to DNA and ARNT. Pre-treatment of PBPs also significantly inhibit B(a)P-induced DNA adduct formation, level of PGE2 and 8-oh-dG in mouse skin. Conclusions: Black Tea polyphenols exhibit their anti-initiation activity via modulation of AhR, which is a transcriptional regulator of phase-I enzyme and subsequently by decreasing DNA adducts.