4^th International Conference and Exhibition on Pharmacognosy, Phytochemistry & Natural Products August 29-31, 2016 Sao Paulo, Brazil

Norma Rivera DVM, PhD, is a Professor in the Department of Microbiology and Parasitology, Faculty of Medicine at UNAM. She works in the field of medical parasitology studying the problems associated with the treatment and control of parasitic diseases that are still major health problem in many countries around the World. Her research interests include the evaluation of synthetic compounds and natural products with antiprotozoal activity and the pathogenesis of Malaria and Toxoplasmosis.

Hintonia latiflora (Hl) stem bark infusions are use in some rural communities of Mexico to treat malaria, diabetes and gastrointestinal diseases. The efficacy of Hl stem bark methanolic extract (HlMeOHe) in CD1 male mice infected with Plasmodium yoelii yoelii (Pyy) was tested. A 4-day test scheme was used. Oral doses of 1,200, 600, and 300 mg/kg were evaluated; oral chloroquine was used as positive control. Transmission electron microscopy (TEM) was used to identify ultrastructural changes on the asexual intraerythrocytic stages of Pyy treated with HlMeOHe. None treated Pyy-infected mice died between 6 and 7 days post-infection (PI) with parasitemia over 70%. Pyy-infected mice treated with 600 and 300 mg/kg showed a chemosuppression percentage of total parasitemia of 99.23 and 23.66, respectively, animals in both groups died 6 to 7 days PI with parasitemia over 45%. 1,200 mg/kg of the extract showed, in the Pyy-infected mice, a 100% chemosuppression of total parasitemia on 5 days PI and a 23 days survival time with a mean parasitemia of 23.6% at the date of death. Maximum extract dose decreases mice temperature up to 3C. TEM images showed morphological changes of parasite death. The results obtained in this study showed that the infection outcome of Pyy-infected mice is affected by HlMeOHe. Although the stem bark of Hl showed efficacy to treat murine malaria, its chemical composition and toxicity should be studied in detail for the benefit of those who consume it.

Hailiang Lu Currently working as an Supervisor at Infinitus (China) Co. Ltd, China. he has published more than 20 papers in reputed journals.

Oriental medicinal plants are widely used in folk medicine as natural healing remedies with therapeutic effects such as prevention of local infection, inflammation disorders, skin itching or enhancing wound healing. In this study, a recipe, named as Balm Total, was developed as a personal care product base to deliver a range of benefits, including antimicrobial, anti-inflammatory, itching-relieving and wound-healing. It involves the use of several herbal extracts, such as Glycyrrhiza glabra, Sophora flavescens, Polygonum cuspidatum, Mentha haplocalyx and Origanum vulgare. Balm Total was found to prevent the growth of microorganisms yeast (Candida albicans), gram-negative (Pseudomonas aeruginosa and Escherichia coli) and gram-positive bacteria (Staphylococcus aureus). The bacteriostatic rates on both the bacteria and yeast were above 99%, showing promising antibacterial and antifungal activities. In the xylene-induced ear-swelling test in mice, Balm Total suppressed the ear swelling (p<0.05). The licking response induced by 4-aminopyridine was significantly reduced by 66.8% (p<0.01). A rabbit ear trauma model was used to evaluate the performance of Balm Total. Percentage of wound area reduction (PWAR) of total number of wounds at Day 5 and Day 11 was 25% and 98%, respectively (p<0.01). Wound healing rate was 33.3% at Day 5 and 75% at Day 11 (p<0.05). The pathological examination demonstrated that at Day 11, the vascularized area was significantly larger in Balm Total treated group than in blank control, fibroblast proliferation was activated and the epidermis demonstrated thickening of stratum spinosum. These results suggest that Balm Total has antimicrobial, anti-inflammatory and antipruritic effects, and could accelerate wound healing

Morgan H J N and bachelor in the course of Biological Sciences, Faculty of Sciences of UNESP, Bauru. Trainee in Physiology of the Endocrine Pancreas Laboratory, in which develops scientific initiation project.

Cancer is a multifactorial disease caused by uncontrolled proliferation of cells due to intrinsic and extrinsic factors of the body, causing an imbalance in the body homeostasis, often leading to the development of cachexia, a metabolic syndrome associated with an increase of mortality and morbidity in patients with neoplasia. The solid Ehrlich tumor-bearing mice (SET) are an experimental model that leads to the development of cachexia. The butanolic fraction of V. tucanorum contains mainly triterpenoids, compounds known for their action on the cancer. Therefore, the study aimed to evaluate the effect of the extract (200 mg/kg, 14 days) on metabolic parameters in cachectic mice with SET. For this four experimental groups (n=10, male Swiss mice) were used-CS (control treated with saline), CV (control treated with extract), TS (tumor treated with saline) and TV (tumor treated with the extract) and sacrificed on day 15 for analysis of serum levels of albumin, total protein, triglycerides, total cholesterol, and tumor, spleen, liver, kidney and adipose tissue weights. The extract did not show acute toxicity. The TV group showed a significant increase in adipose tissue weight, and serum albumin, total cholesterol and total protein levels in relation to TS. Furthermore, the TV group showed a significant decrease in tumor and spleen weights, and serum triglyceride levels when compared to the TS group. Based on these results, we conclude that the treatment with the extract has improved some typical parameters of cancer associated cachexia.

Lei Xiao currently working as a Manager at Infinitus (China) Co. Ltd, China. he has published more than 20 papers in reputed journals

Oriental medicinal plants are widely used in folk medicine as natural healing remedies with therapeutic effects such as prevention of local infection, inflammation disorders, skin itching or enhancing wound healing. In this study, a recipe, named as Balm Total, was developed as a personal care product base to deliver a range of benefits, including antimicrobial, anti-inflammatory, itching-relieving and wound-healing. It involves the use of several herbal extracts, such as Glycyrrhiza glabra, Sophora flavescens, Polygonum cuspidatum, Mentha haplocalyx and Origanum vulgare. Balm Totoal was found to prevent the growth of microorganisms yeast (Candida albicans), gram-negative (Pseudomonas aeruginosa and Escherichia coli) and gram-positive bacteria (Staphylococcus aureus). The bacteriostatic rates on both the bacteria and yeast were above 99%, showing promising antibacterial and antifungal activities. In the xylene-induced ear-swelling test in mice, Balm Total suppressed the ear swelling (p<0.05). The licking response induced by 4-aminopyridine was significantly reduced by 66.8% (p<0.01). A rabbit ear trauma model was used to evaluate the performance of Balm Total. Percentage of wound area reduction (PWAR) of total number of wounds at Day 5 and Day 11 was 25% and 98%, respectively (p<0.01). Wound healing rate was 33.3% at Day 5 and 75% at Day 11 (p<0.05). The pathological examination demonstrated that at Day 11, the vascularized area was significantly larger in Balm Total treated group than in blank control, fibroblast proliferation was activated and the epidermis demonstrated thickening of stratum spinosum. These results suggest that Balm Total has antimicrobial, anti-inflammatory and antipruritic effects, and could accelerate wound healing.

Luciana Jankowsky was graduated in Pharmacy from the Methodist University of Piracicaba, and specialization in Clinical Pharmacology at Methodist University of Piracicaba and master's degree in Cell Biology and Structural at State University of Campinas. She integrates a research group at the Multidisciplinary Center for Chemical, Biological and Agricultural Research. Luciana has experience in the area of Research and Development Cosmetic and Clinical Research, through development of research protocols and preparation of relevant documentation to drug registration Affairs Regulatory, cosmetics and supplies. Currently she is graduate student in Wood Technology, at ESALQ / USP, with a focus on pyrolysis and its products in the areas of preservatives for cosmetics and cleaning products, by techniques of gas and liquid chromatography, high-performance, coupled to mass spectrometry

The wood of several tropical trees species, with abundant secondary compounds present in their extractives, demonstrate potential for phytochemical and pharmacological studies searching natural products with industrial interest; due its potential as a source of bioactive compounds composts. Vatairea Paraensis is one of those species, presenting a high content of extract in the wood. The crudes extracts (polar and polar) and ten different fractions obtained from crude polar extract were tested in vitro, an anticancer drug screen utilizing a panel of 7 human tumor cell lines organized into subpanels, representing melanoma, and cancers of the lung, colon, kidney, ovary and breast. The fraction named F6 obtained a TGI (total grown inhibition) varying from 11,04% (ovary) to 214,44% (prostate) and, based on those results, it was selected for phytochemical isolation and for in vivo hollow fiber assay. HPLC techniques and GC/MS enabled to identified a presence of 3- methyl-1, 8, 9-antracenotriol, or crisobarin, which was confirmed by NMR. In hollow fiber assay, the fibers with OVCAR-03 line were introduced into the peritoneum and subcutaneous of BALB/C mice anesthetized with CO2. The treatments, saline (10 mL/kg), doxorubicin (3 mg/kg), F6 (at doses of 3, 5 and 7 mg/kg) were administered intraperitoneally for 21 days. The results demonstrate the potential antitumor activity of the V. Paraensis F6 fraction for hormone dependent tumor, with an estimated low toxicity, since no animals died. This allows inferring that the search of new chemical compounds could be a tool contributing to rational use and management of the Brazilian rainforest. Biography Luciana Jankowsky has expertise in clinical pharmacology, Masters in Cellular Biology at the Unicamp, and is currently a doctoral student in forest products technology, ESALQ / USP. It has experience in over 12 years in studies of natural products with antitumor employability and, more recently, with antimicrobial activity for use in cosmetics and sanitizing products.

Victor Sotero got Doctorate at Biochemistry Pharmaceutical at FCF- USP; He is the Director of the Circle of medicinal Plants from Amazonian of Foundation for the Sustainable in Lowland Amazonian (FUNDESAB-Peru). He has published more than 30 papers in several scientific journals.

The aim of this study was to realize the evaluation of the allelopathic activity of 83 vegetable species from Allpahuayo – Mishana Reserve in Peruvian Amazon, and to determine the main polar components of three species of that showed high activity. Leaves samples were collected, which were subjected to elution for two weeks to get the methanol extracts e to test the inhibition of the roots of pre-germinated seeds of Lactuca sativa. These extracts were dried in a rotary evaporator and the product subjected to column fractionation opened using silica gel No. 100, using as mobile phase methanol and obtaining the fractions according to the appropriate retention time, and meet the fractions containing similar molecules through analysis of thin layer chromatography; which were tested to evaluate their allelopathic activity against pre-germinated seeds of Lactuca sativa. In this way it was found that three species showed activity in extracts, these were the Iryanthera ulei, Duroia hirsuta and Theobroma obovatum. When performing the analysis on GC-MS. was found compounds as terpenes, phenolics and organic acids, as the following: isoeugenol, catechol, humulene in I. ulei; limonene, geranic acid, neric acid, homovanillil alcohol in D. hirsute; phenol. 2,4-bis (1.1-dimethylethyl), α ionone in T. obovatum and phytol in each.

Garcia de Sotero got Doctorate at Food Sciences at FCF- USP. She is the Principal Professor of National University of Peruvian Amazon (UNAP) - Iquitos-Perú. She has published more than 15 papers in several scientific journals and coauthor of Handbook of Food Chemistry Analysis.

In this study, the allelopathic and antioxidant activity of 32 plant species from Tamshiyacu Tahuayo Communal Reserve were evaluated. For alleopatics was used fresh leaves and extracts metanholics were preparated and added to plates with pregermianted seeds of lactuca sativa, at differents concentrations (0.1 – 50.0 mg/ml). It was determinated the hypocotyl inhibition porcentagem. For the antioxdante evaluation, it was realized by means of the scaening of free radical of DPPH, with the metanholic extracts of the dry leaves different concentrations (0.1-50.0 mg/ml).The results indicate high percentages of allelophatic inhibition at six species (Sapium sp., Xylopia enthami,, Guatteria hyposericea, Tapirira guianensis, Virola surinamensis and Malouetia nauias), achieving more than 50%, and its effective concentration 50% (EC50) is at minimum 0.1 mg / ml. The chemical components of allelopathic activity responsible is due, according to the analyzes, the presence of phenols, anthocyanins, catechins and proantocianidoles. About antioxidant potential the results indicates that three specieds presented high values of inhibition percentage. They were Virola sebifera, Caryocar glabrum and Tapirira guianensis, had the highest percentage of inhibition of 62.75%, 74.25% and 57.40%. The chemical components varied as follows: phenolics (11521.7-32181.7 mg/100g), flavonoids (103.1-144.3mg/100g) and proanthocyanidins (0.063-0.177 mg/100g).

Chougouo Kengne R.D has completed her PhD; She is a Pharmacist Officer and Researcher (CER) at the University Mountain Cameroon. She has published more than 20 papers in reputed journals.

Artesunate is a semi-synthetic derivative of artemisinin, the active compound of the Chinese herb Artemisia annua. It is a drug commonly used to treat malaria, especially chloroquine resistant malaria. Past works on A annua show many hepatoprotectives effects of the plant. Further to these works, the present study was aimed at investigating the fractions in charge of A annua’s antihépatotoxics activities and, searching the activities of artesunate on rat’s hepatocytes. The antioxidants activities of flavonoïds extract and artesunate was assessed by antiradical and reduction experiments. Free radical scavenging activity was determined by measuring the decrease in the visible absorbance of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and artesunate showed an EC50 >100 µg/ml. This result was lower than the flavonoïds one (EC50 = 8, 23 ± 0, 56 µg/ml). Likewise, reduction test showed a poor activity of artesunate. Hepatoprotectives activities were analyzed on two hepatitis models induced. Hepatocyte’s viability according to the extract’s concentrations was studied by the MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) test , the estimation of ALT activities and the MDA level. There was no significant difference between poisoned cells (by CCL4 and Paracetamol) and cells treated by artesunate contrary to aqueous extracts and flavonoïds extract which showed a significant difference (p<0,001). The outcome suggests that Artesunate toxicity may possibly cause damages to the hepatocytes and liver function; effect contrary to that of A annua, who keeps cells alive. A. annua has Hepatoprotective effect.

Ain Raal has graduated as a Pharmacist at the University of Tartu (Estonia), and has completed his PhD at the Institute of Chemistry and Pharmacy of Leningrad (Soviet Union). He is the Head of the Institute of Pharmacy, University of Tartu, Estonia, and the Associate Professor of Pharmacognosy of the same Institute. He has published 80 scientific papers in international scientific journals indexed by the Web of Science, Scopus and other databases, more than 130 conference abstracts, as well as 22 textbooks, monographs, handbooks and popular-scientific books such as ‘Pharmacognosy’ and ‘Encyclopedia of Medicinal Plants of the World’.

Vitamin C is a well-known antioxidant, the deficiency of which leads to scurvy, accompanied with osteoporosis and severe anemia. During the Leningrad Blockade (1941-1944), the scientists worked to extract vitamin C from Scots pine (Pinus sylvestris L.) needles to cure and prevent the outbreaks of scurvy. Since 1930, it was believed that leaves of cowslip (Primula veris L.) contain up to 5.9% ascorbic acid and one leaf was enough to fulfill the recommended daily intake of vitamin C. Such enormous content of ascorbic acid could be the result of titrimetric assay technique used in those days. In the present study, the content of ascorbic acid was determined by slightly modified European Pharmacopoeia HPLC method for quantification of related substances in ascorbic acid. Actually, the content of ascorbic acid in P. sylvestris needles was just from 0.0005% to 0.0067% in dry material. In fresh cowslip, leaves were collected with weekly interval with the content of ascorbic acid varied from 1.2 to 2.4%. The recommended daily intake should be 20-30 fresh cowslip leaves. Spinach, parsley and rucola are poor sources of vitamin C; practically unreal amount of their fresh salad (300-500 g) can fulfill the recommended daily intake of vitamin C. Commercial orange juices contain ascorbic acid from 9-50 mg/100 ml, depending on producer one to five glasses of juice could fulfill the recommended daily intake of vitamin C. On chromatograms of Goji berries (Lycium spp.), we were not able to identify the peak of ascorbic acid.

CRISTIANO MACUAMULE completed graduate studies in Mozambique; Masters studies at Queensland University, Australia and PhD in Biochemistry at Stellebosch University, South Africa in 2014. Cristiano is a lecturer at Universidade Eduardo Mondlane (UEM) in Mozambique. He has been teaching Pharmacology and Toxicology, and supervising graduate and postgraduate students at UEM and externaly. His research interests are in drug discovery from synthetic and natural sources including medicinal plants and, antimicrobial drug resistance. Cristiano has published in reputed journals and serves as reviewer of projects in two national research agencies.

Psydrax locuples is a plant traditionally used to treat infectious diseases and disorders including pain and fever. Scarce scientific information is available to confirm the significance of the uses of the plant. This study investigated the antimicrobial activity of aqueous and ethanol extracts of shade-dried leaves using the disc diffusion test. The analgesic activity was evaluated using the hot plate and the acetic acid-induced writhing tests in albino mice. The yeast-induced fever assay was used to investigate the antipyretic activity of the plant. The extracts showed significantly (p<0.05) better antimicrobial activity against Gram-positive (Bacillus cereus, Staphylococcus aureus, Streptococcus sp, Corynebacterium sp) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria, and the fungus Candida albicans, compared to commercial recommended antibiotic and antifungal drugs. Both extracts administered orally inhibited writhing in dose-dependent manner. The ethanol extract inhibited writhing by 58.63% at 200 mg/kg. The same dose of both extracts showed significant analgesic activity in the hot plate test, 30, 60, 90 and 120 minutes after administration. Both extracts reduced yeast-induced pyrexia, and the temperature of mice administered the highest dose (400 mg/Kg of ethanol extract) was significantly below the normal body temperature of control animais, 2 hours post-treatment. Pyrexia was controlled to up to 5 hours post treatment. These findings demonstrated the antimicrobial, analgesic and antipyretic activity of P. locuples, thus justifying its extensive use in traditional medicine in Mozambique. Further studies are required to investigate the activities of constituent phytochemicals against various ailments, and ensure developments into clinical use

Ezzat Abdel-Lateef has completed his PhD at the age of 33 years from Faculty of Science, Ain Shams University, Cairo, Egypt in August 2014 and postdoctoral studies from Theodor Bilharz Research Institute, Giza, Egypt. He is a Researcher of Medicinal Chemistry at Theodor Bilharz Research Institute. He has published more than 11papers in international journals

The present study was designed to identify the chemical constituents of the methanolic extract of Curcuma longa L. rhizomes and their inhibitory effect on a hepatoma cell line. The methanolic extract was subjected to GC-MS analysis to identify the volatile constituents and the other part of the same extract was subjected to liquid column chromatographic separation to isolate curcumin. The inhibition of cell growth in the Hepatoma cell line and the cytopathological changes were studied. GC-MS analysis showed the presence of fifty compounds in the methanolic extract of C. longa. The major compounds were ar-Turmerone (20.50 %), β-sesquiphellandrene (5.20 %) and curcumenol (5.11 %). Curcumin was identified using IR, 1H and 13C NMR. The inhibition of cell growth by curcumin (IC50 = 41.69 ± 2.87 μg mL–1) was much more effective than that of methanolic extract (IC50 = 196.12 ± 5.25 μg mL–1). Degenerative and apoptotic changes were more evident in curcumin-treated hepatoma cells than in those treated with the methanol extract. Antitumor potential of the methanolic extract may be attributed to the presence of sesquiterpenes and phenolic constituents including curcumin (0.051 %, 511.39 μg g–1 dried methanol extract) in C. longa rhizomes

Prashant Singh has completed his Master in Pharmacy (Pharmaceutics) in the year 2012 from Rajiv Gandhi University of Health Sciences, Bangalore (Karnataka), India. He is pursuing his PhD in Pharmaceutics from Suresh Gyan Vihar University, Jaipur (Rajasthan) India. Currently he is working on formulation and dose designing of Herbal Antidiabetics. He is working as Assistant Professor at Department of Pharmacy, Institute of Technology & Management, GIDA, Gorakhpur (U.P.), India-273209. He has published more than 15 papers in reputed journals and has been attended 7 National and 3 International conferences

India is the land of spices. These spices have been used as traditional medicine in India from long back. Earlier it was only upto the domestic use. From few years it has been recognized worldwide. The World Health Organization (WHO) has listed 21,000 plants, which are used for medicinal purposes around the world. Among these 2500 species are in India, out of which 150 species are used commercially on a fairly large scale. India is the largest producer of medicinal herbs and is called as botanical garden of the world. The current review focuses on medicinal use of spices in various diseases as well as showing antimicrobial activity and also having Insect repellent properties

Fuad had graduated and got B.Sc. in chemistry, University of Salahaddin (2008), he awarded as a best student with rank three in a class of fifty students. He completed, M.Sc. in Natural product Chemistry field, in April 2011, at the age of 29 years old from University of Pavia (Italy), under the supervision of Full Prof. Giovanni Vidari. On the other hand, he is a PhD student in split side program between University of Salahaddin, Erbil, Kurdistan Region and University of Pavia-Italy according to the memorandum between both Universities. He had published papers in reputed journals with high impact factors. Also he had participated in many conferences

The Kurdistan Region of Iraq has a centennial tradition in the use of medicinal plants; however, the majority of them are still uninvestigated from a phytochemically and pharmacologically point of view. Therefore, recently, we decided to start an ambitious project on the isolation and characterization of main bioactive compounds from Kurdish medicinal plants. Within this program, we report, for the first time, the main constituents isolated from Pterocephalus nestorianus Nab. (Dipsacaceae), known in Kurdistan with the name of Lawa. This plant grows in sunny, dry, rock crevices, mostly in Western Asia (Iran, Turkey), and it is used in Kurdistan for treating oral diseases and inflammation. Pterocephalus species are, indeed, widely used as ornamental plants and in several folkloric medicines all around the world. Dry flowers and roots of P. nestorianus (100 g) were separately exhaustively extracted by maceration at room temperature in solvents of increasing polarity: hexane, EtOAc, MeOH, MeOH-H2O, 70:30. Subsequently, chlorophylls were removed from the MeOH extracts by SPE technique. The chlorophyll free extracts were then separately fractionated by repartition between solvents and repetitive MPLC on successive C-18 reversed phase columns. The structures of isolated compounds were established by 2D NMR spectroscopic methods and, in case of known compounds, by comparison with literature. The major bioactive isolated products were kaempferol glycosides and three iridoids: loganic acid, loganin and the new dimer, secologanyl ester. These iridoids show a characteristic high anti-inflammatory activity, which thus corroborate the traditional uses of the plant

Will be updated soon.

This study aims to determine the antioxidant and anti-inflammatory property of commonly used Ghanaian vegetables namely Corchorus olitorius, Solanum melongena, Solanum torvum, Xanthosoma sagittifolia and Abelmoschus esculentus. Inhibition of Nitric oxide produced by LPS induced RAW264.7 macrophage, flavonoid content, total phenolic, glutathione content, phytochemical screening and HPLC fingerprint of freeze dried samples were carried out. Anti-inflammatory activities were determined by measuring the inhibition of nitric oxide production in lipopolysaccharide induced RAW 264.7 macrophages. Their cytotoxicity against macrophages was determined by MTT assay. Antioxidant activities were evaluated by four assays: an antioxidant activity assay using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay to assess free radical scavenging. Total phenolic, flavonoid contents, glutathione contents were determined using the Folin-Ciocalteu and aluminium chloride methods, O-Phthaldehyde method respectively. Presence of saponins, alkaloids, tannins, terpenoids and cardiac glycosides were determined. All the samples showed inhibition of NO production at the concentration of 100µg/ml. Corchorus olitorius showed the highest NO inhibition recording 78.00%, followed by S. torvum X. sagittifolia S. melongena A. esculentus (72.11%, 52.32%, 29.18% and 25.81%). C. olitorius recorded the highest flavonoid content followed closely by X. sagittifolia, A. esculentus, S. torvum and S. melongena (2.915, 2.785, 1.470, 0.846, 0.134 mg/ml in 5mg/ml of each sample respectively). S. torvum recorded the highest phenolic content followed closely by C. olitorius, X. sagittifolia, and S. melongena A. esculentus (2.307, 1.749, 1.637, 1.294, 1.001 mg/ml in 5mg/ml of each sample respectively). A. esculentus recorded the highest GSH content followed closely by S. melongena, S. torvum, X. sagittifolia, and C. olitorius. Solanum torvum exhibited strong free radical (DPPH) scavenging antioxidant activity with an EC50 value of 0.466mg/ml in 10mg/ml sample. Phytochemical constituent were present in all of the samples except terpenoids in S. melongena and A. esculentus. The inhibition of NO production may be due to these. These selected vegetables could be a rich source of antioxidants and free radical scavenging compounds.

Faramarz Jalili was born in 6 Feb 1995 & He is doctorate of pharmacy student in pharmacy school of Kermanshah university of medical sciences in the age of 21 . He has patented up two inventions in IRI Patent Organization. He has published one book in Medical Mycology.

Morphine consumption can decrease fertility drive in males through inducing oxidative stress and DNA damage. Anethum graveolens is a multipurpose herb in traditional medicine for which some anti-oxidative and anti-inflammatory properties have been identified. This study was set to investigate whether the Anethum graveolens could be inhibited morphine adverse effects on reproductive parameters. This experimental study was conducted under approval of Ethics Committee of Kermanshah University of Medical Sciences. In this study fifty six BALB/c male mice with weight range of 25-30 g were purchased from Tehran Razi Institute. The animals were maintained on a 12 h dark/light cycle at about 22 ± 3 oC and allowed free access to standard laboratory diet (Pars Co.) The mice were randomly assigned to 8 groups (n=7). The control group was administered saline (1 ml/kg) and experimental groups were administered morphine (10 ml/kg), Anethum graveolens (150, 300, and 600 mg/kg) and Anethum graveolens (150, 300, and 600 mg/kg) plus morphine (10 ml/kg) interaperitoneally (IP) for 28 consequent days and reproductive parameters were determined. The results indicated that morphine administration (0.5 mg/kg) significantly decreased testosterone level, count and motility of sperms, and testis weight compared to control group (p<0.01). However, increasing the dose of Anethum graveolens significantly increased reproductive indices in most of the groups (p<0.01). Thus, it seems that Anethum graveolens inhibits morphine-induced adverse effects on reproductive parameters.

Will be Updated soon.

This study was focus on the extraction, compositional analysis, anti-mycobacterium tuberculosis and antioxidant determination of essential oil obtained from Bosweillia dalzielii, Ocimum americanus, Hyptis spicigera, Hyptis suaveolens, Eucalyptus cammaldulensis, vossia cuspidata, Lavandula officinalis and Cinnamondedrone cubenes plants with the sole aim of having an ati-tuberculosis and antioxidant preparation from plants oil. The essential oils were extracted from the plants using modified steam distillation technique and their compositions analyzed using Gas Chromatography coupled to Mass spectroscopy of which the results reveal the present of significant number of total identifiable components as follows: for the essential oil of Bosweillia dalzielii; α-Pinene (18.515 %), isophthaladehyde (10.695 %), Ocimun americanus; Terpnene-4-ol (14.507 %) and copaene (7.438 %), Hyptis spicigera; α-pinene (30.536 %) and β-pinene (15.840 %), Hyptis suaveolens; caryophyllen (20.643 %) and Sabinene (16.711 %), Vossia cuspidata ; 4-Acetyl-7-hydroxybenzo-2,1,3-thiadiazole (15.037 %) and α-caryophyllen (11.397 %), Eucalyptus camaldulensis; M-cymene (19.74 %), α-phellandrene (19.280 %) and Eucalyptol (13.990 %), Lavandula officinalis; β-myrcene (33.233 %) and Germacrene (24.747 %) and Cinnamondedrone cubenes; P-mentha 2,5- diene-7-ol trans- (31.257 %) P-mentha 2,5-diene-7-ol cis- (9.195 %). The anti-mycobacterium tuberculosis activities were tested using mycobacterium bovi in which the essential oils of Hyptis spicigera (MIC 0.45% ) and Vossia cuspidata (MIC 0.45 %) have high anti-mycobacterium tuberculosis activities. The antioxidant activities of the essential oils of 8 plants were investigated using 2, 2-Diphenyl-1-picryl hydrazyl (DPPH), the result shows that all the samples have exhibited antioxidant activities with values above 80 %. It has been established in this work that certain essential oils posses both anti-mycobacterium tuberculosis and antioxidant activities, therefore, it is recommended that the potentials of these plants essential oils should be channel for use as raw materials in pharmaceutical industries.

I validated my (PhD) thesis in 2014; I am currently Assistant lecturer in the Department of Pharmacognosy, Botany and Cryptogamie in the University of Félix Houphouët Boigny. My research works focus mainly on metabolic diseases, especially diabetes. I have written a dozen of research papers on diabetes, Phytochemical screening and micrograph of medicinal plants.

Phytochemical analysis and determination of acute toxicity were performed on the leaves of Ocimum gratissimum Linn,which revealed the presence of the following chemical compounds: saponin, sterol, terpene, alkaloids, polyphenols, tannins and flavonoids and especially mucilage. The evaluation of total polyphenols and total flavonoids gave 11.98 ± 0.07 GAE mg/g (mg of Gallic Acid Equivalents (GAE)/g of extract) of dry extract and 3.99 ± 0.57 mg QE/g (mg of Quercetin Equivalents (QE)/g of plant extract) of dry extract respectively. The physicochemical analysis carried out has revealed the following : moisture content 8.97 ± 0.01%, total ash 8.45 ± 0.02 %, Sulfuric ash 10.27 ± 0.01%, ash insoluble in hydrochloric acid of 0.96 ± 0.01%. The determination of mineral salt composition has revealed the presence of Cu, Zn, K, Ca and Mg.These elements play important role in glucose metabolism. The acute toxicity study showed that the toxic dose is above 3000mg/kg. It is also a very good food for human consumption. The study of leave’s extract of Ocimum gratissimum Linn.has revealed that it contains minerals salt, bioactive primary and secondary metabolites which explain why they are been used in the treatment of diabetes mellitus.