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DR. AMBERKAR MOHANBABU VITTALRAO

DR. AMBERKAR MOHANBABU VITTALRAO

MANIPAL UNIVERSITY (FORMERLY KNOWN AS MAHE

Title: Hepatoprotective activity of aqueous extract of Curcuma longa against Anti - tubercular drugs (ATT) induced hepatotoxicity in Wistar albino rats

Biography

Biography: DR. AMBERKAR MOHANBABU VITTALRAO

Abstract

Objective:  To  evaluate  the  hepatoprotective  activity  of  Curcuma  longa  (Cl)  against  ATT

induced hepatic damage in rats.

 

Materials and methods: The rhizomes of the plant were shade dried, powdered and extracted with water. Total 5 groups were used for the study wherein, Gr. I rats were treated with normal saline  as  a control  (vehicle),  Gr.  II and  III  were  given  with  hepatotoxic  ATT  drugs,  isoniazid (INH) 31.5 mg/kg + rifampicin (RIF) 54 mg/kg + pyrazinamide (PZA) 189 mg/kg and silymarin

50mg/kg as standard hepatoprotective drug respectively.   Gr. IV and V rats dosed with 100 and

200  mg/kg  of  Cl  extract  respectively.  The  plant  Cl  extract,  ATT  drugs  and  silymarin  were prepared  as  suspensions  with  2%  gum  acacia.  Calculated  daily  dosage  of  (Cl  extract  +ATT drugs) were split and administered twice for 90 days. The Aqueous extracts of Cl and silymarin were  administered  orally  every  morning  along  with  ATT  drugs  for  90  days.    On  90th   day, biochemical analysis was done by measuring serum liver enzymes, albumin and total proteins levels.   The   hepato-protective   activities   of   Cl   extract   were   further   determined   by   the histopathological examination.

 

Results:  A.  biochemical  analysis:  ATT  treated  rats  showed  significant  increase  in  AST (aspartate    aminotransferases),    ALT    (alkaline    liver    transferase),    ALP    (alkaline    liver phosphatases), and GGT (γ-glutamyl transferase) in the serum Vs. control (P<0.05).  Treatment of silymarin and Cl (100 and 200 mg/kg)  extract showed hepatoprotective activity against the hepatic  damage  induced  by  ATT.  This  was  evident  from  significant  reduction  in  serum  liver enzymes levels ALT, AST, ALP, GGT and also there was significant increase in serum albumin and  total  proteins  as  compared  to  the  ATT  treated  groups  (P<0.05).  B.  Histopathology:  ATT treated  rats  histopathological  slides  revealed  mild-moderate  fibrosis,  low  grade  inflammation, hepatocellular  necrosis  with  sinusoidal  dilatations  suggestive  of  congestion  and  significant hepato-protective changes were found when Cl extract was treated with ATT drugs.

 

Conclusions: The hepato-protective action combined with antioxidant activity has a synergistic effect  to  prevent  the  process  of  initiation  and  progress  of  hepatocellular  damage.  Thence,  we conclude  that  aqueous  extract  of  Cl is  a  promising hepato-protective  agent  could  be  clinically useful when added with ATT drugs.