Malleswara Rao Peram
Central Research Laboratory, MM Group of Institutions, India
Title: Enhanced cellular uptake and cytotoxic potential of curcumin loaded nanovesicular formulations on A375 human melanoma cells
Biography
Biography: Malleswara Rao Peram
Abstract
Statement of the Problem: Melanoma is the most fatal and life threatening type of skin cancer with increasingly higher rates of occurrence worldwide. Although it accounts for 1% of all skin cancers, most of the skin cancer related deaths (75%) occur due to melanoma. Despite extensive clinical research, only few drugs have been approved by United States Food and Drug Administration (USFDA) for melanoma therapy. Most of these drugs kill normal healthy cells besides cancer cells. At present, the overall success rate of current modalities for this chemoresistant and metastatic melanoma is rather restricted. Hence there is an intense need for natural compounds, which can precisely treat melanoma with little or no toxicity on normal cells and prevent its progression to metastatic stage. The objective of the present study is to develop, optimize and evaluate curcumin loaded nanovesicular formulations for the treatment of melanoma. Methodology: The curcumin loaded nanovesicles (ethosomes and transfersomes) were prepared by cold and thin film hydration method respectively. The prepared nanovesicles were evaluated for size, zeta potential, entrapment efficiency, in vitro skin permeation and deposition studies. The optimized formulations were evaluated for in vitro cytotoxicity and cellular uptake studies using A375 human melanoma cells. Findings: The optimized nanovesicular formulations were found to be spherical and unilamellar structures having nanometric size range and high entrapment efficiency. The curcumin loaded nanovesicles showed significant skin permeation and deposition in the skin layers. The fluorescence microscopy study confirmed the enhanced penetration of nanovesicles into the deeper skin layers. The in vitro cytotoxicity and cellular uptake studies revealed that curcumin ethosomes had significantly improved cytotoxicity and cellular uptake in A375 human melanoma cell lines. Conclusion & Significance: The present study provides a strong rationale for further investigation of newly developed curcumin nanovesicular formulations as a potential therapeutic strategy for melanoma treatment
Curcumin loaded nanovesicles: Formulation, characterization, in vitro cytotoxicity and cellular uptake studies against A375 human melanoma cell lines
Recent publications (minimum 5)
- Mirzaei H, Naseri G, Rezaee R, Mohammadi M, Banikazemi Z, Mirzaei HR, Salehi H, Peyvandi M, Pawelek JM, Sahebkar A (2016). Curcumin: A new candidate for melanoma therapy?. International journal of cancer. 139(8):1683-1695.
- Zhang YP, Li YQ, Lv YT, Wang JM (2015). Effect of curcumin on the proliferation, apoptosis, migration, and invasion of human melanoma A375 cells. Genetics and Molecular Research 14(1):1056-1067.
- Yu X, Du L, Li Y, Fu G, Jin Y. (2015). Improved anti-melanoma effect of a transdermal mitoxantrone ethosome gel. Biomedicine & Pharmacotherapy, 73, 6-11.
- Sun Y, Du L, Liu Y, Li X, Li M, Jin Y, Qian X (2014). Transdermal delivery of the in situ hydrogels of curcumin and its inclusion complexes of hydroxypropyl-β-cyclodextrin for melanoma treatment. International Journal of Pharmaceutics. 469(1):31-39.
- Faisal W, Soliman GM, Hamdan AM (2018). Enhanced skin deposition and delivery of voriconazole using ethosomal preparations. Journal of Liposome Research. 28(1):14-21