Rashida Bashir
Swinburne University of Technology, Australia
Title: Untargeted Metabolic Profiling Of Antioxidants within Australian Native Quandong and Desert lime with RF-PCD- FRAP and LC-ESI-MS
Biography
Biography: Rashida Bashir
Abstract
Conventional online Post Column Derivitisation FRAP assay is laborious and time consuming task. Here, we present a rapid Post Column derivitisation FRAP assay of Quandong (Santalum acuminatum) and desert lime (Citrus glauca) with reaction flow chromatography that has reduced the analysis time without compromising the separation performance. This rapid post column derivitisation is achieved using Active Flow Technology-Parallel Segmented Flow (AFT-PSF) columns. The direct feed of FRAP reagent into the outlet fitting of the reaction flow chromatography column eliminates the need for mixing T-pieces, and reduces the post-column extra-dead volume to no more than required in chromatography using standard modes of detection. Antioxidant profiling of Quandong and desert lime samples with an extraction and sonication method has shown an interesting analysis. Extracts were analysed via multiplexed detection using an AFT-PSF column with underivatised UV-VIS, and the Ferric reducing antioxidant potential (FRAP‚) derivatisation for antioxidants as detection methods. Multiplex detection has allowed obtaining substantial data within single analysis. Non-targeted metabolomics with RF-PCD-FRAP and LC-ESI-MS analysis implies that Australian native Quandong and Desert lime are a rich source of antioxidants. Analytes were identified by MS and MS2 with the ESI mass spectra under the same conditions in both positive and negative ionization modes. Samples have exhibited superior antioxidant capacity and comprise predominantly of flavonols, anthocyanin, phenolic acids and their hydrolysable tannins and contains antioxidants with known therapeutical potential in cardiovascular, neurodegenerative and other chronic diseases that play a major role in the prevention/delay of oxidative stress mediated diseases.