John Thomas
VIT University, India
Title: Nutraceutical based treatment of Vibrio anguillarium infection in Macrobrachium rosenbergii
Biography
Biography: John Thomas
Abstract
Vibrio species are the most prevalent pathogens in the aquatic ecosystem. It causes huge mortality. V. anguillarum and V. vulnificus exhibit extensive virulence in marisne vertebrates or invertebrates. Vibrios remain prominently opportunistic but none the less they are largely primary and secondary pathogenic invaders of shrimps present in farms across the globe. This study aims at identifying the pathogenicity of V. angullarium species in n Macrobrachium rosenbergii and developing an effective treatment against the infection. The pathogenecity of M. rosenbergii to V. anguillarum was confirmed by intramuscular injection. The clinical symptoms such as lethargy, redish and green colouration on the body surface were observed. The highest concentration of V.angularium caused 100% mortality within 98 hrs of post inoculation when injected intramuscularly. However the lower concentrations of V. angularium caused 45% and 40% mortality, after 120 and 96 h of post injection respectively. The LD50 value of V. angularium for intramuscular route was found to be 2.4 × 10-4 at 120 hrs of post injection. The present study revealed that 25µl of ethanolic extract of Ocimum tenuiflorum was effective in treating the infection. This can be incorporated in to the feed and used for for treating the infection as an alternative to antibiotics. Resistance to the antimicrobial agents used in aquaculture has increased in many countries in recent years. Medicinal plants are potential and promising sources of pharmaceutical agents against pathogens in aquaculture.
