Ankita Misra
CSIR- National Botanical Research Institute, India
Title: Geographical variation impact on metabolite(s) content and antioxidant activity of Gloriosa superba l. germplasms for identification of elite chemotypes fron Central India and Gangetic plains (India).
Biography
Biography: Ankita Misra
Abstract
G. superba L., (Colchicaceae) is a highly valued medicinal plant due to its high colchicine content, used in both traditional and modern system of medicine. Colchicine, a major chemical marker in this species and the content varies from 0.7 to 0.9 %. The present study aims for HPTLC quantification (% dry wt.) of alkaloid (colchicine and gloriosine) and poly phenolic markers (kaempferol and quercetin) in G. superba through validated method. The antioxidant potential of test extract(s) was evaluated and further correlated with its metabolites content. 17 germplasm was collected from different sites, morphotypic variation was not observed within them although the content of bioactive metabolites varies significantly. Maximum content of colchicine and gloriosine was reported in NBG-10 (Kanth, U.P) and NBG- 11 (Mohanlalganj, U.P) having 0.513 and 0.165% which was not reported earlier in Indian population. Quercetin and kaempferol content varies from 0.0007 to 0.122 % and 0.005 to 0.075 %, maximum is reported in NBG-13 (Bheragha, M.P) germplasm. Polyphenolics in test extract(s) reflect the promising antioxidant activity with significant correlation to TPC and TFC. Hence, seven germplasm were found as elite chemotypes of G. superba (NBG-1, NBG-10, NBG-11, NBG-12, NBG-13, NBG-14 and NBG-17) in the targeted phyto-geography based on bioactive metabolites and in vitro bioassay. This aids in identification of chemical variation existing within the species inhabiting in the same phytogeographic conditions, to locate the high yielding germplasm source and for promotion of commercial cultivation to meet out industrial demand.