Selfie P.J. Ulaen
Health Polytechnic Manado, Indonesia
Title: Effect of ethanol concentration seed extract papaya (carica papaya l.) In inhibits growth of Escherichia coli
Biography
Biography: Selfie P.J. Ulaen
Abstract
Papaya is known as a multipurpose plant for almost all the plants useful to humans, one of which is the papaya seeds are used as medicine roundworms, indigestion, diarrhea, skin diseases, male contraception, and raw materials into the wind. This study aims to evaluate the efficacy and the effect of different concentrations of 1%, 3% and 5% ethanol extract of papaya seeds in inhibiting the growth of bacteria Escherichia coli.
The study was a laboratory experiment with post-test design with control group. The research sample using black papaya seeds from the village Tompaso II District Tompaso Minahasa North Sulawesi Province. The ethanol extract of papaya seeds obtained from the maceration of 50 grams of dried papaya seeds powder using ethanol 70%, then made a test solution of each concentration of 1%, 3% and 5%. Test of inhibition is done by using a paper disc soaked at each concentration, and then placed on a nutrient agar which had been inoculated with the bacterium Escherichia coli, and then incubated in an incubator at a temperature of 37â°C for 1 x 24 hours, 2 x 24 hours and 3 x 24 h and the diameter of clear zone formed was measured using a scale ruler mm. The data were analyzed descriptively continued with One Way Anova.
The results showed difference in the effectiveness and influence of the concentration of 1%, 3% and 5% ethanol extract of papaya seeds in inhibiting the growth of bacteria Escherichia coli.