Nedhal Aldouri
Al-Ahliyyah Amman University, Jordan
Title: Biogenetic conversion of antimicrobial phytoalexin wyerone into wyerone epoxide and their antibacterial activity from Vicia faba plant cotyledons
Biography
Biography: Nedhal Aldouri
Abstract
The biogenetic conversion between the antimicrobial phytoalexins in the broad bean plant cotyledons, wyerone (I), 11-12- dihydrowyerone ( II) ,and wyerone epoxide (III) with their antimicrobial activity has been investigated
Labeled wyerone and dihydrowyerone were obtained by feeding sodium (2- 14C) acetate to abiotically CuCl2- induced Vicia faba cotyledons, and separation by HPLC.
Results
The results in this study indicated that wyerone epoxide was derived from wyerone, and dihydrowyerone transformed into wyerone epoxide quite efficiently, but the epoxidation of wyerone to wyerone epoxide appeared to be more important. It is possible that a metabolic grid existed for these compounds in V. faba.
⇒Wyerone ⇒Wyerone epoxide⇒Dihydrowyerone⇒Wyerone epoxide
An Antibacterial activity
Antibacterial activity of the separated phytoalexins from cotyledons of Vicia faba was screened against human pathogens ( Bacillus cereus, Klebsiella pneumonia), plant pathogens ( Curtobacterium flaccumfaciens, Pseudomonas syringae, Rhodococcus fascians) and a saprophyte ( Bacillus megaterium) covering Gram positive and gram negative bacteria using disc diffusion method. The three phytoalexins showed good antimicrobial activity against Gram positive bacteria while no antimicrobial activity against Gram negative bacteria was exhibited.
Conclusion
The origin of wyerone biosynthesis in Vicia faba was studied previously (Al-Douri,et al., 1986) and the conversion of wyerone to wyeron acid has been demonstrated earlier ( Al-Douri, 2014).In the present study the conversion of wyerone into wyerone epoxide using labeled compounds and the potential rule of these compounds in the biogenetic relationship as well their potential antibacterial activity has been established.