Rambir Singh
Bundelkhand University, India
Title: Antidiabetic effect of Carissa carandas in Rats and the Mechanism of its Insulin Secretagogues activity in Isolated Pancreatic Islets
Biography
Biography: Rambir Singh
Abstract
Carissa carandas (CC) has been documented as a traditional treatment for diabetes. In the present study, the CC fruit aqueous, methanol, chloroform and ethyl acetate extracts were examined for hypoglycemic activity in healthy Wistar rats. Aqueous Extract of CC (AECC) showed highest fall of 67.08% in fasting blood glucose from 0 to 1h in glucose tolerance test (GTT). The ED50 of AECC was 300mg/kgbw in streptozotocin induced diabetic rats. Treatment of diabetic rats with ED50 of AECC for 28 days significantly reduced post prandial glucose (PPG) by 33.65% (p<0.01), glycosylated hemoglobin (HbA1c) by 45.79% (p<0.01) and increased insulin level by 69.7% (p<0.05). The increase in insulin secretion may be partly responsible for antidiabetic effect of AECC. To assess the mechanism of secretagogues activity, AECC was incubated with isolated pancreatic islets of Wistar rats at basal (3.3mM) and high (16.7mM) level of glucose in presence or absence of Diazoxide (K-ATP channel opener), Nimodipine (Ca2+ Channel blocker) and Calphostin-C (PKC inhibitor). AECC induced insulin secretion at 16.7mM of glucose was significantly (p<0.01) reduced by Diazoxide and Nimodipine but non significantly (p>0.05) by Calphostin-C. The study indicated that the phytochemicals present in AECC may be inducing insulin secretion by closing K-ATP channels in β-cells of pancreatic islets.