Cikra Ikhda Nur Hamidah Safitri
Academy of Pharmacy Mitra Sehat Mandiri, Indonesia
Title: Antibacterial, Antimycobacterium And Citotoxicity Activities Of Tin Fruit (Ficus Carica) Compounds In Java
Biography
Biography: Cikra Ikhda Nur Hamidah Safitri
Abstract
Tin Fruit (Ficus carica) is one of famous fruit in Indonesia. The antibacterial and citotoxicity test of pure compounds of Ficus carica, which was collected from Sidoarjo, East Java, Indonesia have been done. Isolation and purification of the crude extracts and the pure compounds were carried out using several chromatographic techniques. The structures of the isolated compounds were elucidated by spectroscopic methods such as UV, IR, 1D (1H, 13C, DEPT) and 2D (COSY, HMQC, HMBC) NMR and MS. Four compounds, identified as β-sitosterol (1), 6-(2- methoxy-Z-vinyl)-7-methyl-pyranocoumarin (2) and 9,19- cycloarlane triterpenoid (3) were isolated from this plant. These compounds were screened for their antibacterial and cytotoxicity activities. The significant antibacterial activity were shown by compounds and against Staphylococcus aureus and Bacillus subtilis bacteria also Mycobacterium tuberculosis and Mycobacterium murinum in high concentration. Meanwhile, the screening for cytotoxicity using SRB assay on MCF-7, SKOV3, HT-29 and MDA-MB-231 cell lines for these compounds revealed the percentage of cells survival at doses 15 μg/ml were higher than 50 %.