Mona H Hetta
Fayoum University, Egypt
Title: The amoebicidal potential of Arachis hypogaea L. pericarp on cysts of Acanthamoeba astronyxis T7 genotype
Biography
Biography: Mona H Hetta
Abstract
Acanthamoeba is an opportunistic pathogen causing keratitis and fatal encephalitis. Early diagnosis, followed by aggressive treatment using a combination of drugs is a prerequisite for successful treatment. Many natural compounds have demonstrated lethal effects, yet the search for novel natural amebicidal agents is still of current interest. The study investigated the in vitro amoebicidal effect of A. hypogaea L. (peanuts) pericarp (as waste product); resveratrol, total methanol extract and its fractions (n-hexane, dichloromethane, ethyl acetate and ethanol) on cysts of A. astronyxis T7 genotype from patients suffering keratitis. Acanthamoebae were isolated, cultivated on 1.5% non-nutrient agar and incubated with different concentrations of the plant extractives. The total methanol extract showed the highest mean of non-viable (cysts 99%), followed by the ethanol, ethyl acetate, dichloromethane, n-hexane extractives and finally resveratrol regarding accumulative effect on second day. The study highlighted a guide for the best concentration of each extractive to be used and the duration and effect it will give along accumulation. All the used samples proved in vitro amoebicidal activity and could be considered new promising natural agents with special regards to the synergetic effect of different constituents in total methanol extract which added to its potency.