Yalda Shokoohinia
Kermanshah University of Medical sciences, Iran.
Title: Isolation of novel polyacetylenes from Echinophora cinerea and investigation of their cytotoxicity on PC3, SKNMC and MCF-7 cell lines
Biography
Biography: Yalda Shokoohinia
Abstract
Introduction: Echinophora cinerea belongs to Apiaceae family. Its aerial parts are used as vegetables and seasoned yogurt and cheese, and for the treatment of digestive disorders in Chahar Mahal and Bakhtiari. Despite the traditional use and dietary and pharmacological studies on Echinophora spp, no attempt has been made to isolate secondary metabolites of its non-polar (acetone) extract. So phytochemical investigation seems to be necessary to use this plant in a better manner. Methods: Powdered aerial parts of the plant were macerated with acetone and concentrated extract was fractionated on RP-18 sorbent using mixture of methanol and water with decreasing polarity. The resulting fractions were analyzed by NMR and promising fractions were refractionated and purified using normal phase column chromatography and reversed and normal phase preparative HPLC analyses and structures of pure compounds were determined by HNMR, COSY, HSQC, HMBC and NOESY spectra and Mass analysis. Results: After extraction, column chromatography and HPLC purification of acetone extract, three novel skeleton polyacetylene compounds (echinophorin A-C) were resulted. Cell cytotoxicity of pure compounds was evaluated by MTT assay on MCF-7, SKNMC and PC3 cell line. Echinophorin A and B show significant cytotoxicity effect on PC3 and SKNMC cell line and IC50 of these compounds are 23 and 25 µg/ml on PC3 repectively. Conclusion: Regarding novel skeleton constituents of E.cinerea, this plant could be a good source of potential medicinal natural products. Considering the fact that polyacetylenes are natural protective compounds, this plant could utilized as an antioxidant agent.