Sujan Banik
Noakhali Science and Technology University, Bangladesh
Title: In vivo antinociceptive, antihyperglycemic and in vitro membrane stabilization activity with phytochemical screening of methanolic extract of Garcinia lancifolia Roxb
Biography
Biography: Sujan Banik
Abstract
Garcinia lancifolia Roxb. is an important unexplored medicinal plant belonging to the family Cluseaceae, which has been used traditionally to treat many diseases. No scientific validation has been made up to now on its pharmacological activity for which the present study was to investigate the antinociceptive, antihyperglycemic activity and in-vitro membrane stabilizing activity with phytochemical screening of methanolic extract of G. lancifolia whole plant. The phytochemical screening confirmed the presence of flavonoids, saponins, alkaloid, cardiac glycoside, terpenoids extensively. In peripheral antinociceptive activity assessment by acetic acid writhing inhibition method, the extract of G. lancifolia (400 and 200 mg/kg) showed significant inhibition of writhing (P<0.001) with 59.15% and 49.30% respectively compared to standard Diclofenac (54.92% inhibition). The central antinociceptive activity by the tail-immersion method, the methanolic extract at dose of 400 and 200 mg/kg showed significant analgesic activity having 78.31% (P<.0.05) and 89.95% (P<.0.01) elongation of reaction time respectively in 90 min after administration of sample compared to the standard Morphine (708.99% elongation). In the hot plate tail-flick method, the extract (300 mg/kg) showed significant (P<.0.05) analgesic activity compared to the standard Aceclofenac. In hypoglycemic activity evaluate by the most acceptable method of glucose tolerance test, the methanolic extract exhibit statistically significant (P<0.001) antihyperglycemic activity at doses of 200 and 400 mg/kg of body weight compared to standard drug Glibenclamide (10 mg/kg) at different time interval. In membrane stabilizing activity assay, clearly evident that the methanolic extracts of G. lancifolia were highly effective to prevent the lyses of erythrocytes induced by heat. During heat induced condition this extract demonstrated 71.35% inhibition of haemolysis of RBC respectively by isotonic solution. The experimental outcomes of the present study revealed that this plant possess noteworthy pharmacological activities that may be basis for further research to find possible mode of action of the plant part.