Domko D F
Université des Montagnes, France
Title: Development of a suppositories pilot batch of leaves and stems of Artemisia annua grown in Cameroon
Biography
Biography: Domko D F
Abstract
Malaria is the most deadly disease that concerns mostly African children under the age of five. Its difficult treatment because of drug resistance to conventional molecules leads to the use of Artemisinin-based Combination Therapy (ACT) recommended by WHO. Several studies, for instance those of Chougouo and al. showed that the infusion of A. annua is more efficient than ACT after 7 days of treatment, but hardly accepted by children mostly those under 2 years old because of the quantity to administer. The present study is to put in place a more acceptable dosage form for children suppositories made from A. annua grown in Cameroon. To evaluate its quality, the powder of leaves and stems of A. annua has been submitted to physicochemical analysis. The particle size conducted by the sieve method and laser diffraction. Artemisinin, determined by TLC - densitometry, then read through MESURIM software. Entire flavonoids titrated by Aluminum chloride. The formula of medicines established and suppositories submitted to pharmacotechnical tests. The powder obtained, of bitter taste, greyish-green, with characteristic odor (camphor), is homogeneous with 56,37 % of particles in the sieve of diameter over or equal to 63 μm. The artemisinin and entire flavonoids contents are respectively of 5 mg/g and 0,43 mg equivalent to quercetin per Gramm of dry matter. 250 mg suppositories of active principle have been made knowing that, 1g of A. annua powder moves 0,72 g of Suppocire C. They are dark-green, shiny, smooth, barrel-shaped. Their average weight is 2,15 g, disintegration time 8 min 16 s, the fusion point 35,7 ˚C. The made A. annua suppositories, are in conformity with european pharmacopoeia. The suppositories will contributed to a better treatment of malaria among children.