SE Mazibuko-Mbeje
Medical Research Council of South Africa, South Africa
Title: Beneficial effect of Aspalathus linearis on hepatic insulin resistance
Biography
Biography: SE Mazibuko-Mbeje
Abstract
Recent studies have reported that plant extracts such as Rooibos (Aspalathus linearis), well-known for its use as herbal tea, could play a potential role in the prevention and treatment of metabolic disease. This study aimed to establish whether aqueous and organic solvent-based polyphenol-enriched extracts prepared from “fermented” (oxidised) (FRE) or “unfermented” (unoxidised, green) (GRE) rooibos (10 µg/mL), respectively, can ameliorate palmitate-induced insulin-resistance in C3A liver cells and the liver of obese insulin-resistant (OB/IR) rats. The major polyhenol in GRE was the flavonoid, aspalathin, a dihydrochalcone unique to Rooibos. Palmitate (0.75 mM) was used to induce insulin resitance in C3A cells. Thereafter, cells (with or without palmitate) were treated with FRE or GRE for 3 h and insulin (1 µM for 15 min). Glucose uptake, palmitate uptake and ATP content were determined. OB/IR rats were subsequently treated at various doses (32, 97 and 195 mg/kg BW) of GRE for 12 weeks to confirm in vitro findings. Body weights and blood glucose concentrations were monitored weekly and fasting insulin concentrations were assessed after 12 weeks treatment. Protein and gene expression relevant to insulin-signalling, AMPK and lipid metabolism were investigated by Western blot in C3A cells and RT-PCR in liver tissue. Insulin resistance in C3A cells was confirmed by a reduction in insulin-stimulated glucose uptake. FRE and GRE reversed the inhibitory effects of palmitate on insulin-stimulated glucose uptake and ATP concentrations. In the OB/IR rat, GRE lowered elevated insulin concentrations and improved insulin sensitivity. Mechanistically, GRE improved expression of genes and proteins that affected glucose and lipid metabolism in vivo and in vitro. This study provides evidence that both FRE and GRE could play role in the amelioration of insulin resistance, in spite of qualitataive and quantitative differences in phenolic compoistion.