Mayuresh Joshi
Ramnarain Ruia College, India
Title: Hepatoprotective and Pharmacokinetic profile of a mangrove: Avicennia marina
Biography
Biography: Mayuresh Joshi
Abstract
Avicennia marina, a pioneering mangrove, reported to be traditionally used against hepatitis, still remains therapeutically unexploited. Investigators have reported several constituents from the plant with prospective medicinal value as chemotherapeutic agents like betulinic acid, taraxerol, etc, but scientific data on its pharmacological usage remains unavailable. Many phytoconstituents found present in this plant have been reported to possess hepatoprotective activity.
In this study, hepatoprotective activity of standardized ethyl acetate extract of leaves has been evaluated. Chromatographic standardization was carried out using validated HPTLC method for simultaneous estimation of five biomarkers namely betulin, betulinic acid, ursolic acid, betasitosterol and lupeol. Safety was established in albino Wistar rats following the OECD guidelines no. 420. Hepatoprotective activity (50, 150 and 300 mg/kg) was investigated in CCl4 intoxicated albino Wistar rats. The results proved concentration dependent percent protection at par with the positive control, Silybon 70. CCl4 produced a marked elevation in the level of plasma biomarkers which were reversed to normalcy by the oral administration of the extract. The results of histopathology were also found to be in compliance with the findings of blood biochemistry. The extract also showed potent antioxidant activity in DPPH and galvinoxyl radical scavenging models.
Further, an HPLC method was developed and validated (USFDA) to study the pharmacokinetics of betulinic acid and ursolic acid (Cmax at 0.20 h and 2.0 h, respectively) from rat plasma. These observations endorse an overall promising hepatoprotective potential against liver disorders using standardized ethyl acetate extract of the leaves of Avicennia marina.