Ikpefan Emmanuel Oise
Delta State University,Nigeria
Title: C-AEDE- Cytotoxic and Antiproliferative Evaluations of a novel diterpenoid from Euphorbia graminea
Biography
Biography: Ikpefan Emmanuel Oise
Abstract
Bioassay guided fractionation of the methanol extract of Euphorbia graminea against breast cancer (MCF-7), lung cancer (NCI-H460) and NIH 3T3 (mouse embryonic fibroblast normal cell line) at 1-250 µg/mL was carried out. Extracts of E. graminea was partitioned into aqueous and chloroform fractions and both fractions were tested for their effects on MCF-7 and NCI-H460. Further chromatographic and biological studies of the active chloroform fraction yielded a compound whose identity was revealed as Abietane-11, 23 diene-16-oic-14-ones through NMR and MS studies. This compound was observed to give –3.3 ± 1.4 and –5.30 ± 3.75 % cytotoxicity against MCF-7 and NCI-H460 at 100 µM with GI50 and TGI of +38 ± 0.74, 96.94±6.95 µM and 53.70±9.30 and 93.88± 11.70 µM respectively. The results has established the rationale for the use in ethnomedicinal practice.