Day 1 :
Keynote Forum
Chandrakant Kokate
K L E University, India
Keynote: Quality considerations for global recognition of Indian herbal medicines: A critical appraisal
Time : 10:05-10:30
Biography:
Chandrakant Kokate is a Professor, Vice-Chancellor, National Advisor. Society of Pharmacognosy
Abstract:
India is one of the major producers of herbal raw materials systematically used across the globe for the production of wide spectrum of phyto-medicines. The Indian herbal industry has the potential of capturing the world market provided strategic approach is adopted for developing convincing quality control parameters for the domestic production of herbal medicines. Blessed with rich traditional heritage of Ayurveda, it is possible for India to acquire the status of global leader with the application of science of modern drug technology to the production of herbal medicines. Quality control of raw material in terms of its genuinity, purity and efficacy has to be coupled with the quality assurance profile for the polyherbal formulation manufactured in accordance with statutory guidelines. The process of weeding out of spurious, adulterated and misbranded herbal formulations must be initiated and scientific validation of the indigenous herbal medicines should be prioritized to capture the global market.
Keynote Forum
Dieter Brömme
The University of British Columbia, Canada
Keynote: Natural product-derived ectosteric inhibitors of cathepsins
Time : 10:30-10:55
Biography:
Dieter Brömme received his Doctoral degree from the University of Halle-Wittenberg (GDR) in 1983. Prior to his appointment as Professor at the Faculty of Dentistry at the University of British Columbia, Vancouver and Canada Research Chair in Proteases and Diseases in 2004, he was a Research Officer at the Biotechnology Research Institute in Montreal, Canada, a Project Leader at Khepri Pharmaceuticals in South San Francisco and a Professor at the Mount Sinai School of Medicine in New York. His research interest is focused on mammalian lysosomal cysteine proteases where he published almost 180 research papers and book chapters.
Abstract:
Cysteine cathepsins are potent extracellular matrix-degrading proteases that have been implicated in various skeletal, vascular, and respiratory pathologies. Highly potent active site-directed inhibitors have been developed and tested in preclinical and clinical studies. Whereas they are highly effective in slowing down the destructive matrix degradation process, they also have serious side effects, which can be attributed to the inhibition of the regulatory functions of the target protease. We have developed a strategy where only the pathologically relevant degradation of collagen and elastin is inhibited without interfering with the other proteolytic functions of the protease. These inhibitors are named ectosteric inhibitors, which bind outside of the active site and either block the binding of therapeutically relevant substrates, ligand binding and/or protease oligomerization needed for the degradation of extracellular matrix proteins. Natural product libraries appear to be a rich source of such inhibitors. Using various plant extracts and purified compound libraries, we identified potent ectosteric inhibitors, which specifically inhibit the collagenase and elastase activities of cathepsins. The talk will discuss the strategy of the identification of ectosteric inhibitors, their mechanism, and efficacy in in vivo models. Emphasis will be given to compounds identified in traditional Chinese Herbal Medicine used in skeletal ands cardiovascular diseases.
Keynote Forum
Xulin Chen
Wuhan Institute of Virology, China
Keynote: Germacrone, a major component of the essential oils extracted from Rhizoma Curcuma, inhibits early stages of influenza virus replication
Time : 11:10-11:35
Biography:
Xulin Chen is a professor at Wuhan Institute of Virology, Chinese Academy of Sciences. He obtained his Ph.D. in Microbiology from Institute of Microbiology, CAS, in 1999. He received postdoctoral training at University of Pennsylvania (1999-2003) and Fox Chase Cancer Center (2003-2005) in Philadelphia, USA. His lab focuses on antiviral research, which includes the development of novel antiviral and anti-inflammatory therapeutics from natural products against virus infections. He has published more than 34 papers in reputed journals and has been serving as an editorial board member of Virologica Sinica.
Abstract:
Highly pathogenic influenza viruses pose a serious public health threat to humans. Antivirals are needed to efficiently control disease progression and virus transmission. In this study, germacrone, which is a major component of the essential oils extracted from Rhizoma Curcuma, was found to inhibit influenza virus replication. Germacrone showed antiviral activity against the H1N1 and H3N2 influenza A viruses and the influenza B virus in a dose-dependent manner. The viral protein expression, RNA synthesis, and the production of infectious progeny viruses were decreased both in MDCK and A549 cells treated with germacrone. In a time-of-addition study, germacrone was found to exhibit an inhibitory effect on the early stages of the viral replication cycle. By a defined indirect immunoinfluscent assay of the localization and quantification of HA, NP and M1 proteins of influenza virus. Germacrone was found to inhibit the fusion of virus membrane and the endosomal membrane, thereby blocked the dissociation of the vRNPs from M1 matrix proteins. Germacrone also exhibited an effective protection of mice from lethal infection and reduced the virus titres in the lung. Furthermore, the combination of germacrone and oseltamivir exhibited an additive effect on the inhibition of influenza virus infection, both in vitro and in vivo. Our results suggest that germacrone may have the potential to be developed as a therapeutic agent alone or in combination with other agents for the treatment of influenza virus infection.rn
Keynote Forum
Umesh K Patil
Dr H S Gour Central University, India
Keynote: Metabolomics and ethno-pharmacology: A multidisciplinary approach in novel drug discovery from natural sources
Time : 11:35-12:00
Biography:
Umesh Patil has completed his PhD from Dr. H.S. Gour University, Sagar and postdoctoral studies from Institute of Biology, Leiden University. Presently he is serving as General Secretary to Indian Society of Pharmacognosy. His area of specialization is Herbal Drug Technology and Natural Products. He has more than 15 years of experience in teaching and research. He is recipient of 7 prestigious awards given in the field of HMPs and remained BOYSCAST fellow of DST. He is a member of many professional bodies including International Pharmaceutical Federation (FIP), International Society of Ethnopharmacology(ISE), International Society for Medicinal Plant Research (GA), Germany, International Pharmaceutical Students Federation, International Society of Natural Product Development, APTI, IPA, IPGA, ISTE and IAHP. He has published more than 100 papers in reputed journals and has been serving as editorial board member of many reputed journals.
Abstract:
Phytochemical screening techniques are developing fast and we now have tools available which allow us to analyse complex mixtures in ultra modern ways. The modern research era allows a systematic investigation of such complex mixtures and specifically to link phytochemical analysis with other strategies (such as in vitro or in vivo screening for biological activity or toxicity, morphological plant diversity and ecological parameters). Specifically as it relates to the study of medicinal and food plants, the main challenge is to understand the complex effects of such extracts. Since most of these research activities are linked to plants used in traditional and local cultures, this requires an ethnopharmacological approach coupled with modern metabolomic investigations. The potential of such multidisciplinary approach and its potential benefits for phytochemically oriented research will be discussed. The traditional medicines offer a rich and largely unexplored source of therapeutic leads for the pharmaceutical industry. Academic research and the pharmaceutical sciences may profit in many ways by including ethnopharmacological and metabolomic investigations. One highly illuminating example is the acetylcholine-esterase inhibitor galanthamine. The early research into the botanical species was derived from Galanthus species. It was based on local, but poorly documented knowledge, which was then turned into a medication to treat poliomyelitis and ultimately resulted in a novel treatment of Alzheimer’s disease. rnAlthough herbal medicinal products (HMPs) have been perceived by the public as relatively low risk, there has been more recognition of the potential risks associated with this type of product as the use of HMPs increases. Potential harm can occur via inherent toxicity of herbs, as well as from contamination, adulteration, plant misidentification, and interactions with other herbal products or pharmaceutical drugs. Regulatory safety assessment for HMPs relies on both the assessment of cases of adverse reactions and the review of published toxicity information. However, the conduct of such an integrated investigation has many challenges in terms of the quantity and quality of information. Adverse reactions are under-reported, product quality may be less than ideal, herbs have a complex composition and there is lack of information on the toxicity of medicinal herbs or their constituents. Nevertheless, opportunities exist to capitalise on newer information to increase the current body of scientific evidence. The integration of ethnopharmacology and metabolomics can reduce the uncertainty in decision making with respect to herbal medicinal products.
- Track 2: Analytical Techniques in Phytochemistry
Track 3&11: Herbal Drugs & Formulations, Herbal System of Medicines
Track 4&9: Drugs from Natural Sources, Natural Products of Medicinal Interest
Track 10&12: Crude Drugs & Plant Products, Plant Biotechnology & Tissue Culture
Location: Hyderabad International Convention Centre, India
Session Introduction
Wilfred T Mabusela
University of the Western Cape, South Africa
Title: Phytochemical studies of selected south african medicinal plants
Time : 12:00-12:20
Biography:
Wilfred Mabusela completed his PhD at the University of Cape Town, South Africa, and this was followed by two years of postdoctoral studies at the same institution, which extended his doctoral work on structural studies of plant polysaccharides. He subsequently took up a lecturing position at the University of the Western Cape where he is currently an associate professor. He has published more than 30 papers in reputable journals, and has been a visiting scientist at institutions in Europe and North America.
Abstract:
Traditional medicine is a cultural practice with a long history in South Africa and involves the use of approximately 3000 plants, out of a national biodiversity represented by about 30 000 higher plant species. A number of these medicinal plants have been investigated in our lab for their phytochemical composition, and this paper will describe some of the contributions which we have made in this regard during the last few years. Selected plant species, which included Sutherlandia (Lessertia) frutescens, Galenia africana, Cissampelos capensis, and Gethyllis multifolia among others, were subjected to extraction using a variety of organic solvents followed by chromatographic fractionation of the extracts obtained. Isolated compounds were examined for their chemical structural features with the aid of nuclear magnetic resonance and mass spectroscopy, leading to the identification of compounds belonging to the following classes: alkaloids, flavonoids and, triterpenoid- and flavonoid- glycosides, some of which were of novel character. Some of the crude extracts and purified compounds were also studied for cytotoxicity using the brine shrimp lethality bioassay, and for antimicrobial activity against gram-negative and gram positive bacteria as well as fungal species.
Sneha J Anarthe
Osmania University, India
Title: Sphaeranthus indicus- As natural immunomodulator
Time : 12:20-12:40
Biography:
Sneha has completed her PhD from JNTU, hyderabad in 2012 and having 10 yrs of teaching experience. She is working as Associate Professor in Gokaraju Rangaraju College of Pharmacy, Hyderabad. She has pulished more than 25 research article in reputed journals
Abstract:
The present investigation is to evaluate the immunomodulatory activity for methanolic extract of Sphaeranthus indicus Linn. at different doses as 100, 200 and 400 mg/kg bd.wt. in healthy wistar albino rats. The assessment of immunomodulatory activity was evaluated for humoral immunity (antibody titre, plaque forming cell assay and quantitative haemolysis of SRBC) and cellular immunity (delayed type hypersensitivity, T- cell population and drug induced myelosuppression) with antigen challenge by sheep RBCs. Methanolic extract of Sphaeranthus indicus along with the antigen (sheep red blood cells) showed significant increase in the circulating antibody titer and the number of plaque forming cells (PFC) in the spleen at the dose of 100 mg/kg bd.wt. as compare to 200 and 400 mg/kg bd.wt. Methanolic extract of Sphaeranthus indicus also showed significant (p < 0.01) increase in the DTH response, restoration of histological parameters, increase in lymphocytes and rosettes formation in T-cell population at dose of 400 mg/kg bd.wt. as compare to 100 and 200 mg/kg bd.wt. The present investigation reveals that Sphaeranthus indicus can be used as immunomodulator for activation of specific and non- specific immune responses.
Hassan Abdalla Almahy Dafalla
Taif University, Kingdom of Saudi Arabia
Title: Chemical constituents of the leaves of Ficus nitida and their antimicrobial activity
Time : 12:40-13:00
Biography:
Hassan Abdalla Almahy Dafalla has completed his PhD from University Putra Malaysia. He is the director of a premier Bio-Soft service organization. He has published more than 28 papers in reputed journals
Abstract:
The dichloromethane extract of the air-dried leaves of Ficus nitida belongs to the family Moraceae afforded lutein (1), β-sitosterol (2), stigmasterol (3), chlorophyll a (4), phytol (5), (E)-3-alkenoic acid (6), triglycerides, fatty alcohols, and fatty acids, while the twigs yielded saturated fatty acid ester. The structures of 1-5 on the basis of spectroscopic data such as IR, UV, 1H-NMR, 13C-NMR, COSY, HMQC, HMBC, DEPT and MS and confirmed by comparison data with those reported in the literature. The structure of 6 was elucidated by 1D and 2D NMR spectroscopy. However, bioactivity screening showed that the pure isolated compounds possessed activity on two species of bacteria Bacillus cereus NRRLUI-1447 and Pseudomonas aeruginosa UI-60690 and no activity against four species of fungi (Aspergillus ochraceus NRRL 398, Candida lipolytica ATCC 2075, Sacchromyces cereviseae NRRL 2034 and Sacchromyces lipolytica).
Sathyanarayana B
Muniyal Institute of Ayurveda Medical Sciences, India
Title: Ayurvedic bioenhancers: A classical and contemporary review
Time : 13:30-13:50
Biography:
Sathyanarayana B is an MD in Ayurveda in the subject Bhaishajya Kalpana (Ayurvedic pharmaceutics) from IPGT and RA, Jamnagar. He is currently working as Principal in Muniyal Institute of Ayurveda Medical Sciences, Manipal. He is also working as Research Executive and MR for ISO in Muniyal Ayurveda Research Centre. He has teaching, research, pharmaceutical and clinical experience of 14 years and administrative experience of five years. He has presented more than 40 papers as resource person in various national and international conferences, besides chairing the scientific sessions. He has over 20 publications across various national and international journals besides being a Reviewer for three indexed international journals.
Abstract:
Herbal bioenhancer is an agent of herbal origin or any phytomolecule, which is capable of enhancing bioavailability and bio-efficacy of a particular drug or nutrient with which it is combined, without any typical pharmacological activity of its own at the dose used. Maximizing bioavailability is therapeutically important because the extent of bioavailability directly influences plasma concentrations and consequently therapeutic efficacy. Bioavailability enhancement can make the expensive drugs affordable and reduce the toxic effects by reducing the required dose of drugs. A bioenhancer may act by different mechanisms like, promoting the absorption of the drugs from gastrointestinal tract, inhibiting or reducing the rate of biotransformation of drugs in the liver or intestines, modifying the immune system in such a way that the overall requirement of the drug is reduced substantially, etc. Ayurveda the science of life is based on highly scientific principles. The one of the oldest system of medicine, Ayurveda is momentous in audience of worldwide on virtue of its holistic approach of life. Ayurvedic pharmaceutics is highly advanced explaining over ten thousand formulations under different categories like Kashaya, Vati, Asava-arishta, Avaleha, Paka, Khanda, Bhasma, Pishti, etc. Formulations of Ayurveda consist of substances of herbal, mineral/metal and animal origin which are processed pharmaceutical to have therapeutic effects. Ayurveda explain different processes to enhance the bioavailability of the drugs, e.g., Bhavana, Marana (to produce incinerated products containing nano particles), Snehapaka (to form oleaginous products akin to liposomes), Ksheerapaka, etc. It also explains the concepts like Yogavahi, drugs like Pippali (Piper longum), Shilajatu, Kajjali, Honey, Ghee, etc., that are scientifically established as bioenhancers. Current presentation throws a light on various processes and drugs in Ayurveda improving bioavailability. Their role in the design and development of formulation also will be discussed.
Ranjitsinh Devkar
The M S University of Baroda, India
Title: Anthocyanin rich extract from red cabbage (Brassica oleracea) reduces oxidative stress and prevents apoptosis in stressed rat cardiomyocytes
Time : 13:50-14:10
Biography:
Ranjitsinh Devkar is currently an Assistant Professor at the M S University of Baroda, India. His primary focus is on atherosclerosis and related lifestyle disorders. His work focuses on bioactive compounds of ethnomedicinal origin and their mechanism in alleviating the said diseases in relevant experimental models. He has about 70 publication and conference presentation history and is expanding research into three main areas viz. anthocyanins to alleviate atherosclerotic inflammation and myocardial necrosis, use of functional foods in mitigating atherosclerosis, and finding novel therapeutants in an endemic ethnomedicinal Indian herb.
Abstract:
The colourful bioflavonoids are highly publicized for their free-radical scavenging and antioxidant capacities and form the basis of preventive medicine. Anthocyanins have been reported to be protective against a myriad of human diseases including myocardial infarction, atherosclerosis and cancer. This study assesses therapeutic potential of anthocyanin rich extract of Brassica oleracea (Red cabbage; ARCE) against experimentally induced oxidative damage in H9C2 (rat cardiomyocytes) cells through a carefully scripted protocol. ARCE was prepared and validated using standard procedures and H9C2 cells were pre-treated with the same (250 µg/ml) for 24 h. Later, cells were subjected to H2O2 (100 µM) induced oxidative stress (12 h). Cytotoxicity assay showed significantly less (5.86%) mortality in ARCE+H2O2 treated group as compared as compared to the H2O2 group (48.44%). Also, H2O2 treatment accounted for intracellular high oxidative stress and lower mitochondrial membrane potential. The same was comparable to control values in ARCE+H2O2 treated groups. Also, the expression of antioxidant (SOD and CAT), apoptosis (BAX and Bcl2) and cardiac marker (Caveolin 3) genes were assessed that further corroborated protective role of ARCE against H2O2 induced cardiotoxicity. ARCE pre-treatment also accounted for decreased indices of apoptosis in ARCE+H2O2 (19.1%) group as compared to H2O2 treated group (57.3%). These observations are first report on cardioprotective potential of anthocyanins from ARCE that add further value to Brassica oleracea as a functional food.
Dipal Gandhi
Nirma University, India
Title: Anticancer potential of Dillenia indica and Dillenia pentagyna plants and its correlation with presence of active phytoconstituent
Time : 14:10-14:30
Biography:
Dipal Gandhi has completed her BPharm and MPharm from L M College of Pharmacy, and PhD from Nirma University, Ahmedabad Gujarat. She is working as an Assistant Professor, Department of Pharmacognosy at Institute of Pharmacy, Nirma University since last 8 years. Her area of research work is mainly in phytochemical investigation, standardization and quality control of herbal drugs, analytical method development of phytoconstituents and evaluation of their pharmacological activity.
Abstract:
The present investigation was aimed to develop simple, sensitive, fast, and accurate RP-HPLC method for estimation of betulinic acid from bark and leaf of D. indica and D. pentagyna, widely distributed in forest regions of India, is used as a traditional medicine for the treatment of cancer and cardio vascular diseases. HPLC separation was achieved on a C18 Purosphere Star Hyber (250x4.6 mm, 5 μm, Merck). The mobile phase consisting of acetonitrile and water (93:7 v/v) with flow rate 1.2 ml/min at 207 nm. For estimation of betulinic acid, different fractions such as benzene and ethyl acetate using bark and leaves of D. indica were prepared. Chromatogram showed peak of betulinic acid at Rt 6.308 min. Analysis of data showed that the method is reproducible and selective for the estimation of betulinic acid. Based on results obtained by RP-HPLC method, prepared fractions were evaluated for its anticancer potential. MTT assay has been performed on three different cell lines HCT-15, DU145 and A-375 which explains role of betulinic acid and the anticancer potential of plants.
Preety Panwar
University of British Columbia, Canada
Title: Anti-osteoporotic cathepsin K inhibitors from Salvia miltiorrhiza
Time : 14:30-14:50
Biography:
Preety Panwar completed her PhD and is a Postdoctoral Fellow in Dieter Bromme’s group at University of British Columbia. She has completed her PhD entitled “Targeted and sustained drug delivery using liposomal formulations” from G B P U A T University, India in February 2011. Currently, she is investigating the mechanism of cathepsin K mediated extracellular matrix degradation, and identification of specific anti-collagenase inhibitors of cathepsin K and their in vitro and in vivo evaluation. She has published many papers in reputed journals.
Abstract:
Inhibition of osteoclastic Cathepsin K (CatK) is of major interest for the development of antiresorptive drugs and the treatment of osteoporosis. In this study, we propose ectosteric inhibitors that specifically block the collagenase activity of CatK without interfering with its catalytic site. We discovered three compounds (T01, T04, and T06) which differentially inhibit the in vitro CatK-mediated degradation of soluble and fiber collagen. Here, we demonstrate that T06 is the most potent ectosteric inhibitor of CatK for both soluble and fiber collagen degradation whereas T01 and T04 are comparatively weaker inhibitors but appear to discriminate between the inhibition of soluble and fibrous collagens. The bone antiresorptive efficacy of these compounds was analyzed in human osteoclasts assays and compared with the highly potent active site-directed inhibitor, odanacatib. None of the compounds affected the metabolic activity and viability of osteoclasts. T01 and T06 as well as odanacatib strongly inhibited the overall bone resorption, the generation of CatK-released collagen fragments (CTx-assay), and abolished the formation of trenches by about 55% for the resorption surface, 50% for resorption depth, and 63% for CTx release. On the other hand, T04 exhibited no effect on the formation of trenches and release of CTx, which may indicate a metabolic inactivation of the compound in the osteoclast assay. Our observations show that ectosteric inhibitors extracted from Salvia miltiorrhiza have a similar potency as active site-directed inhibitors of the protease and may constitute a novel approach for anti-resorptive therapy.
Sandip Mali
Pitambari Products Pvt.ltd, India
Title: Preparation of stable & potentized gomutra formulation “Gomutra plus capsule†w.r.t conventional forms of gomutra
Time : 14:50-15:10
Biography:
Will be uploaded soon
Abstract:
The science of natural alternative medicine, Ayurveda (meaning: the ‘knowledge of life’) originated in India. Use of naturally-occurring materials for therapeutic as well as prophylactic purposes is characteristic to the practice of Ayurveda. Gomutra / Cow urine has been elaborately explained in Ayurveda and described in "Sushruta Samhita", "Ashtanga Sangraha" and other Ayurvedic texts as an effective medicinal substance/secretion of animal origin with innumerable therapeutic properties. Bhav- Prakash Nighantu describes gomutra as the best of all types of animal urine (including human) and enumerates its various therapeutic uses. Gomutra is called "Sanjivani" and "Amrita" in Ayurveda. In spite of having so many medicinal properties Gomutra is not used efficiently because of limited access and availability of cow’s urine for regular mass consumption, unpleasant the taste and odor ,stability of gomutra, loss of bioactive component during processing. To Overcome these limitation Gomutra is converted to powder form under extremely low pressure & high temperature of short duration which allows to restore bioactive as present in Gomutra. Comparitive physic-chemical analysis also reveals Bioactive wise superiority of Gomutra powder w.r.t Ark & Ghanavati. By further enrichment with herbs Amala(Embelica officinalis), Harada(Terminalia chebula), Behada (Terminalia balerica); Gomutra Plus capsules has advantages of Synergistic combination of Gomutra powder and Triphala extract; Stabilized, odor-free, concentrated, and potentized formulation of Gomutra; Higher efficacy in small pharmaceutical doses.Though it improves the stability but also preserve bioactive composition of Gomutra at greater extent, unlike to Ark & Ghanavati.
Pramod Hurkadale
K L E University, India
Title: In vitro studies on Achyranthes coynei Santapau. for micropropagation
Time : 15:10-15:30
Biography:
Pramod H J is the Professor and Head, Department of Pharmaceutical Biotechnology, K L E University’s College of Pharmacy, Belagavi, India and has completed his BPharm degree from Kuvempu University, Shivamogga obtained his MPharm degree with distinction from Rajiv Gandhi University of Health Sciences, Bangalore. He was awarded PhD in Pharmacy from Rajiv Gandhi University of Health Sciences, Bengaluru under AICTE sponsored quality improvement program. He has also been invited as Speaker for international conferences at South Africa, Malaysia and Egypt. He is has been awarded Batch of the Best Award from Zydus-Indon Cadila Health Care Ltd, Ahmedabad. His areas of research interest are herbal formulations/excipients, bio-surfactants, traditional medicines and its screening for various pharmacological activities and jointly collaborated with Regional Medical Research Centre, ICMR, Belagavi on tissue culture studies of Medicinal Plants from Western Ghats region.
Abstract:
Achyranthes coynei Santapau., a rare, endemic medicinal plant was successfully propagated in vitro using apical shoot tips as explants. A combination of 3.0 mg.L-1 of 6- Benzylaminopurine (BAP), 0.5 mg.L-1 Thidiazuron (TDZ) and 0.5 mg.L-1 Kinetin (KN) in Murashige and Skoog (MS) agar-solidified medium proved the best combination for multiple shoot induction, yielding 408 shoots per explants after 60 days of culture. The shoots were rooted with a frequency of 80% after 30 days of culture on MS agar medium fortified with IBA/NAA (0.5 mg.L-1) before being acclimatized in the green house.
Radhakrishna G Pillai
University of Calicut, India
Title: Curcumin prolongs graft survival in mouse corneal allografts.
Time : 15:30-15:50
Biography:
Radhakrishna G Pillai has completed his PhD from the Department of Biochemistry, University of Kerala and postdoctoral studies from School of Medicine, University of California, San Dieg, USA (200-2001) and Imperial College London (2001-2007). Now he is working as Assistant Professor at the Department of Life Sciences, University of Kerala. He has published more than 30 papers in reputed journals and has been serving as an editorial board member of various reputed journals.
Abstract:
Curcumin is a dietary pigment from the plant Curcuma longa with known antioxidant, antineoplastic and anti-inflammatory effects. It is quite safe and has been ingested by people for centuries without significant reports of intoxication. Acute and subchronic toxicology studies showed limited adverse effects. The immunosuppressive and graft protective effects of curcumin were studied in mouse corneal transplant models. Curcumin significantly increased the mean survival time (MST) to 32 days, as compared to 14 days among non treated controls. After transplantation there was significant increase in the level of chemokines and cytokines in corneal tissue, which was reduced by an ip treatment with 50mg/kg curcumin for 7 days. On treatment with curcumin a decrease in the expression level of inducible Nitric Oxide Synthase (iNOS) mRNA and production of nitric oxide by the corneal tissue were also observed. This study for the first time demonstrates the effectiveness of curcumin as a novel immunosuppressant after corneal transplantation. This also reveals the protective effect of curcumin on graft survival.
Giuliana Vila Verde
Universidade Estadual de Goiás, Brazil
Title: Optimization of the extraction process for quality control of essential oils obtained from the fruit of Pterodon emarginatus (Fabaceae) occurrent on Brazilian Savana
Time : 15:50-16:10
Biography:
Giuliana has completed PhD in 2011 and her postdoctoral studies in Molecular Biology from the Federal University of Goiás in 2014, directed by Prof. Dr. José Realino de Paula. She is currently Professor of Pharmacognosy holder at the State University of Goiás and collegiate member of two Sensu strict Masters Courses as Molecular Sciences and Territories and Cerrado Cultural Expressions. Her researches have emphasis on Ethnobotany, phytochemistry, quality control, extraction of compounds by irradiation of microwave and biological assays of Savanas´medicinal plants specially Erythroxylaceae and Fabaceae. Member of the Multidisciplinary Network for Sustainable Production Animal Pro-Midwest, Brazil, foccusing on optimization and extraction of organic compounds for the development of bioproducts.
Abstract:
The Pterodon emarginatus specie (Fabaceae) is popularly known as "white sucupira" or "faveiro" that grows on Brazilian Savana. Under Brazilian folk medicine its fruits are used mainly for their antinociceptive properties, anti-inflammatory, antimicrobial and antioxidant. Fruit´s oil and extracts of bark and stems have antimicrobial, anti-inflammatory, analgesic, antirreumática and help control diabetes. Thus, it is used on the treatment of muscle aches, sprains, arthritis and arthrosis. In the Midwest region, the population take stem bark´s tea for gynecological infections.The reference method for the extraction of essential oil is by Clevenger apparatus, and the hydrodistillation enhanced by microwave method to be tested and potentially replaceable. The goal was to optimize the essential oil extraction technique of the fruits of sucupira by irradiation of microwave and characterize the volatile compounds with a view to quality control and species identification. The fruits of sucupira were subjected to oil extraction second literature´s protocols. The process conditions were established in Delineation Central Composite Rotational (CCRD) and the results were evaluated by applying Response Surface Methodology (RSM). Waste analysis was performed at 95% confidence limit (p <0.05), which consisted of the significance test of model fit, based on the analysis of variance (ANOVA). Reaction time and moisture conditions were standardized for these analyses. Under these conditions, the yield is 6.6% which sets the optimum adaptation of procedures to the model. So our objective of optimizing the extraction method was hit. The results are important to contribute to stablishment of quality control parameters of this drug and also, getting less time, saving energy and solvents, which puts us in line with green chemistry
Umesh K Patil
Dr H S Gour Central University, India
Title: Metabolomics and ethno-pharmacology: A multidisciplinary approach in novel drug discovery from natural sources
Biography:
U K Patil has completed his PhD from Dr H S Gour University, Sagar and Postdoctoral studies from Institute of Biology, Leiden University. Presently, he is serving as General Secretary to Indian Society of Pharmacognosy. His area of specialization is Herbal Drug Technology and Natural Products. He has more than 15 years of experience in teaching and research. He is recipient of 7 prestigious awards given in the field of HMPs and remained BOYSCAST Fellow of DST. He is a Member of many professional bodies including International Pharmaceutical Federation (FIP), International Society of Ethno-pharmacology (ISE), International Society for Medicinal Plant Research (GA), Germany, International Pharmaceutical Students Federation, International Society of Natural Product Development, APTI, IPA, IPGA, ISTE and IAHP. He has published more than 100 papers in reputed journals and has been serving as Editorial Board Member of many reputed journals.
Abstract:
Phytochemical screening techniques are developing fast and we now have tools available which allow us to analyze complex mixtures in ultra modern ways. The modern research era allows a systematic investigation of such complex mixtures and specifically to link phytochemical analysis with other strategies (such as in vitro or in vivo screening for biological activity or toxicity, morphological plant diversity and ecological parameters). Specifically, as it relates to the study of medicinal and food plants, the main challenge is to understand the complex effects of such extracts. Since most of these research activities are linked to plants used in traditional and local cultures, this requires an ethno-pharmacological approach coupled with modern metabolomic investigations. The potential of such multidisciplinary approach and its potential benefits for phytochemically oriented research will be discussed. The traditional medicines offer a rich and largely unexplored source of therapeutic leads for the pharmaceutical industry. Academic research and the pharmaceutical sciences may profit in many ways by including ethno-pharmacological and metabolomic investigations. One highly illuminating example is the acetylcholine-esterase inhibitor galanthamine. The early research into the botanical species was derived from Galanthus species. It was based on local, but poorly documented knowledge, which was then turned into a medication to treat poliomyelitis and ultimately resulted in a novel treatment of Alzheimer’s disease. Although Herbal Medicinal Products (HMPs) have been perceived by the public as relatively low risk, there has been more recognition of the potential risks associated with this type of product as the use of HMPs increases. Potential harm can occur via inherent toxicity of herbs, as well as from contamination, adulteration, plant misidentification, and interactions with other herbal products or pharmaceutical drugs. Regulatory safety assessment for HMPs relies on both the assessment of cases of adverse reactions and the review of published toxicity information. However, the conduct of such an integrated investigation has many challenges in terms of the quantity and quality of information. Adverse reactions are under-reported, product quality may be less than ideal, herbs have a complex composition and there is lack of information on the toxicity of medicinal herbs or their constituents. Nevertheless, opportunities exist to capitalize on newer information to increase the current body of scientific evidence. The integration of ethno-pharmacology and metabolomics can reduce the uncertainty in decision making with respect to herbal medicinal products.
Rajiv Dahiya
President, Association of Pharmacy Professionals (APP), India
Title: Complex cyclic oligopeptides: Synthesis and biological properties
Time : 16:25-16:45
Biography:
Rajiv Dahiya is Doctor of Science in Clinical Pharmacology from International University for Complementary Medicine, Colombo, Sri Lanka and also holds PhD in Pharmacy from Uttar Pradesh Technical University, Lucknow, Uttar Pradesh, India. He is presently President of Association of Pharmacy Professionals (APP), Editor-in-Chief of international level journal - Bulletin of Pharmaceutical Research (BPR) and Principal at Globus College of Pharmacy, Bhopal, Madhya Pradesh, India. He has 13 years of teaching and 10 years of research experience. His research area is synthetic peptide chemistry and until now, he has published 51 research papers and 9 review articles in various international and national journals covering a total impact factor of 36.6. He is the recipient of ‘Innovative Researcher Award’ in Jun 2012; ‘Excellence Award’ in Pharmacy in Feb 2014; ‘Young Pharmacist Award’ & ‘Young Scientist Award’ in Mar 2014; ‘Distinguished Pharmacy Professional Award’ in Oct 2014 and ‘Young Investigator Award’ in Mar 2015.
Abstract:
Natural products are the active components not only of most traditional medicines but also many modern medicines. They have pharmacological or biological activity that can be of therapeutic benefit in treating disorders. Some current medicines are obtained directly from natural sources but other medicines are developed from the natural product lead originally obtained from the natural source. Among wide range of natural products, complex cyclic oligopeptides peptides are of special interest for their enhanced stability and pharmacologic properties. Due to their limited conformational flexibility, cyclic peptides with C-to-N-terminal peptide bond and a disulfide bridge can confer high target binding affinity and resistance to proteolytic enzymes. Peptides and proteins are attractive initial leads for the rational design of bioactive molecules. Several natural cyclic peptides have recently emerged as templates for drug design due to their resistance to chemical or enzymatic hydrolysis and high selectivity to receptors. Further, bicyclic peptides can bind with high affinity and selectivity to protein targets, making this format attractive for biotechnological and medicinal applications. The good binding properties are based to a large extent on the limited conformational flexibility of the two connected peptide rings. On the other hand, Host Defense Peptides (HDPs), small cationic peptides, play a vital role in innate immunity response and immuno-modulatory stimulation. Antimicrobial Peptides (AMPs) like β-defensins and cathelicidin, are involved in the defence against pathogenic organisms, e.g., the antimicrobial activity of saliva largely depends on histidine-rich AMPs (histatins). These complex cyclic congeners being isolated from higher plants and marine resources, possess modified amino acid residues like DHHA, ADHA, AHOA, AHMP and exhibit their pharmacological properties through binding to corresponding enzymes which allow cyclic oligopeptides to act as therapeutic agents in resistant world.
Suresh V Chennupati
Mother Teresa Pharmacy College, India
Title: Traditional medicine (Ayurveda): Inspired approaches to drug discovery
Time : 16:45-17:05
Biography:
Suresh V Chennupati has completed his PhD from Acharya Nagarjuna University. He is the Principal of Mother Teresa Pharmacy College, one of the premier colleges in Khammam District. He has published more than 25 papers in reputed journals and has been serving as an Editorial Board Member of repute. He received Bharat Siksha Ratan, International Award for Educational Excellence, APP Young Performer Awards. He is the Co-Author of Pharmaceutical Inorganic Chemistry Text Book Published by Pharma MedPress. He is Executive Editor for an online journal titled Journal of Applicable Chemistry and is also the Andhra Pradesh State Branch President for Association of Pharmacy Professionals.
Abstract:
Ayurveda is an Indian traditional system of medicine. It was originated in ancient India many thousand years ago. The origin, development and practice of Ayurveda have many dimensions and complex theories based on religion, faith and ancient Vedic science. Ayurveda is one of the official systems of medicine in India and it is also widely practiced in many other countries. Considerable research on Pharmacognosy, Photochemistry, Pharmacology and Clinical therapeutics has been carried out on Ayurvedic medicinal plants. Many pharmaceutical companies changed their strategies towards natural product drug discovery and it is important to follow systems biological applications to facilitate the process. Number of drugs are entered the International Pharmacopoeia through the study of ethno pharmacology and traditional medicine. For Ayurveda and other traditional medicines, newer guidelines of standardization, manufacture and quality control are required. Drug discovery strategies based on natural products and traditional medicines are the attractive options. Drug discovery and development need not always be restricted to new molecular entities. Traditional knowledge give an idea for drug development can fallow reverse pharmacology approach and reduce the time and cost of process are expected to takes place mainly from innovation in drug target elucidation and lead structure discovery. Powerful new technologies such as automated separation techniques, high- throughput screening and combinational chemistry are revolutionizing drug discovery. Traditional knowledge can offer smart strategy for new drug candidates to facilitate discovery process and also for the development of rational synergistic botanical formulations.
Muralidhar Rao Akkaladevi
Maheshwara Institute of Pharmacy, India
Title: Nano formulations of bioactive natural products for the treatment of cancer
Time : 17:05-17:25
Biography:
Muralidhar Rao is eminent teacher in Pharmaceutical Sciences. Currently, he is serving as Professor & Principal at Maheshwara Institute of Pharmacy, Patancheru, Hyderabad. He did his B.Pharm, M.Pharm & PhD (Pharmaceutics) from Kakatiya University, Warangal. He received Senior Research Fellowship (SRF) from AICTE, New Delhi; Best Presentation (Oral) Award in National convention of Association of Pharmacy Professionals, Bhopal, India ; “APP Principal of the year Award” at DD university Gujarat; “Bharat Excellence Award at IIC, New Delhi. He is President, Association of Pharmacy professionals, Telangana State branch. He is the fellow member of Association of Pharmacy Professionals (FAPP), International Science congress Association (FISCA), International Congress of Pharmacy and Health Sciences (FICPHS), International Congress of Chemistry and Pharmacy (FICCP). He is life member of Indian Pharmacy Graduate Association (IPGA), Association of Pharmaceutical Teachers of India (APTI), Indian Pharmacological Society (IPS).
Abstract:
According to the NNI, Nanotechnology is the understanding and control of matter at dimensions between approximately 1 and 100 nanometers (nm), where unique phenomena enable novel applications not feasible when working with bulk materials or even with single atoms or molecules. Nanotechnology is ideally suited for drugs with solubility problems .One of the key factors affecting bioavailability of drugs is solubility. Many bioactive natural products have poor water solubility .Nanotechnology has the potential to give new breath of life to those bioactive natural products which were rejected due to their poor solubility. Poorly soluble drugs can also be solubilized by other conventional methods. But those methods have various limitations. The nanocrystal technology enables formulations to be developed without the need of toxic surfactants (e.g. Cremophor EL) which may cause enhanced side effects or adverse reactions. Taxol formulation was very difficult because of hydrophobicity. It is made into liquid formula by dissolving it in a solvent called Cremophor. Many people react badly to this solvent and when administered intravenously experienced neurotoxicity. Abraxis, developed a form of Paclitaxel using nanoparticles of protein called Albumin. This protein can bind bind to receptors in blood vessels. Tumor response rate was twice as good for the nano formulated drug. Only 30 minutes are needed for a complete IV dose of the drug instead of three hours. Thus reduced side effects improved quality of life.
Fatimah Mohammad Budair
University of Dammam School of Medicine, Saudi Arabia
Title: Dermatosis due to Saudi cultural and religious practices that use complimentary and locally prepared remedies
Biography:
Fatimah Budair has completed MBBS from King Faisal University in Dammam ,Saudi Arabia in 2006 and finished the Saudi Board of Dermatology and Dermatological Surgery in 2011. She is a consultant of Dermatology in King Fahad Teaching Hospital of the University, University of Dammam and an assistant professor in Dermatology. She has published different papers in reputed journals and has been serving as a reviewer in the Saudi Journal of Dermatology and Dermatological Surgery.
Abstract:
Saudi Arabia, the largest Arab country of the Middle East, is defined by its Islamic heritage, and its Bedouin traditions. These rich family traditions and practices are handed down from generation to generation. Many of these socio-religious and cultural practices are done using complimentary and locally prepared remedies, but unfortunately these used remedies may not always be as safe as they seem, and cutaneous adverse reactions may occur. The lecture will discuss the habits practiced by people in Saudi Arabia using complimentary and popular natural remedies (henna, kohl, myrrh, honey and applying local remedies for treatment purposes ) and explain the potential secondary dermatomes that may develop, which can be unfamiliar to other doctors from different countries.
- Young Research Forum
Location: Location: Hyderabad International Convention Centre, India - See more at: http://pharmacognosy-phyto
Session Introduction
Srinu Meesala
IISER, India
Title: Isolation, purification, characterization of natural products from marine pseudomonas sps
Time : 17:25-17:40
Biography:
Srinu Meesala has completed his PhD from Andhra University and doing postdoctoral studies at IISER PUNE.
Abstract:
There is a perpetual need for new chemotherapeutants, especially novel antibiotics, to combat new diseases and drug-resistant pathogens that are becoming a significant threat to public health [1]. In spite of the development of chemi-informatics, molecular modeling and other rational drug discovery tools, natural products (NPs) still continue to be a major contributor to new drugs. Over 28% of the new chemical entities and 42% of the anticancer drugs introduced into the market in the last three decades can be traced back to NPs [2]. More than 50,000 microbial natural products (MNPs) have been obtained and have played an important role in drug discovery. However the rate at which new compounds are being discovered from microbial sources is declining. On the other hand, the emergence of severe resistance to antibiotics in microbial pathogens, such as Gram-positive methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant S. aureus (VRSA) increases, there is a need to discovery of MNPs with unique scaffolds to meet the urgent demand for new drugs. Phenazines are redox-active nitrogen-containing aromatic compounds produced by a diverse range of bacterial genera. we isolated Pseudomonas aeruginosa , Genbank accession no. KJ558373, producing phenazines, showing broad spectrum activity and exhibit strong cytotoxic activity against mouse macrophage (RAW 264), human neuroblastoma (SHSY5Y), human lung cancerous (A549), human liver cancer (HEPG2) cell lines with IC 50 value less than 50ng/ml. Chemical characterization studies like UV, IR, HRESIMS, 1D and 2D NMR revealed (1-2) are known phenazines and (3) is a novel phenazine derivative.
Anitha Vadekeetil
Panjab University, India
Title: Role of analytical, in silico and imaging tools in characterisation and activity studies of ajoene from garlic bulbs against biofilms of P. aeruginosa
Time : 17:40-17:55
Biography:
Anitha V completed M.Sc Biotechnology from Bharathidasan University followed by DBT sponsored Advanced PG Diploma in plant genetic engineering from Madurai Kamaraj University with distinction. Presently she is pursuing PhD from Panjab University, Chandigarh. She has published 3 research papers in international journals.
Abstract:
Biofilm of P. aeruginosa impose major health issues due to its resistance towards antibiotics and host immune responses. The postantibiotic era proposes urgent need for alternate strategies to combat biofilms. The relevance of dietary natural products is emerging in this context as they are safe to consume and have no side effects. However, the major concern in this area is the constraints in characterisation and analysis of herbal compounds because of lack of availability of standards and highly complex nature of these compounds. Hence, the present study focuses on applications of analytical, imaging and in silico tools for analysis and activity of ajoene, a novel compound from garlic (Allium sativum L.) bulb extract. Ajoene was extracted by TLC and showed 97.7 % purity by HPLC. Further characterisation was performed by H1 NMR and MS. The amount of ajoene in garlic extract was found to be 221.08 nmol/mg of extract by HPTLC. Results of TLC overlay, HPTLC and docking analysis elucidated the mechanism of action of ajoene against P. aeruginosa. Ajoene was found to exhibit antibiofilm effects which were confirmed by imaging techniques such as confocal laser scanning microscopy and scanning electron microscopy. Thus, the present study throws light on the possible techniques which could be helpful in evaluating the properties of herbal products.
Hina Deepak Mehta
Dadasaheb Balpande College of Pharmacy, India
Title: Milk thistle’s : A boon for multiple therapy
Time : 17:55-18:10
Biography:
Hina Deepak Mehta has completed her M.Pharm at the age of 25 years from rashtrasant tukdoji maharaj nagpur univesity. she is the assistant professor of Dadasaheb Balpande college of pharmacy, Besa Nagpur.
Abstract:
This article reviews on published reports pertaining to milk thistle’s (Sylibum marianum) benefits in the treatment of various diseases. This plant used from ancient times as a hepatoprotective drug. Along the hepatoprotective action of Silymarin, a flavonolignan from‘milk thistle’ was also effective in gastrointestinal disorders and its antitumoral activities, antioxidant, antiinflamatory agent, nephroprotector, radiation and skin protector. silymarin owns also other actions as an, anti-lipid peroxidative, antifibrotic,, immunomodulatory, liver regenerating, etc. It was also studied its neuro-psihiatric and cardiac action. A systematic review of this evidence to clarify what is known and identify gaps in knowledge would be important to guide design of future studies of the mechanisms of milk thistle and clinical trials.
Swastika Maity
Manipal college of Pharmaceutical Sciences, India
Title: Physico-chemical properties of ethno-medicinal plant Hygrophila auriculata and Paederia foetida leaves grown in herbal kitchen garden
Time : 18:10-18:25
Biography:
Swastika Maity is currently studying 4th year B.pharm from Manipal College of Pharmaceutical Sciences, Manipal University, Karnataka. She has presented poster and got the best poster award in Range Management Conference in Kalyani, West Bengal and Pharmaquora ,2015.
Abstract:
Medicinal plants have been used in pharmaceutical practices for thousands of years. India has a wide range of biodiversity and due to its tropical climate which can support large number of ethnomedicinal plant. Hygrophila auriculata and Paederia foetida are such ethno-medicinal plant. H.auriculata is an aquatic perennial herb with spine and P. foetida is a climber which has ovate to lanceolate leaves. As both the plant has wide medicinal values, people and tribes of local area of these plant use their leaves in their diet, the plants with root were collected from the marshy land of West Bengal and grown in herbal kitchen garden at Jhansi , which has semi arid tropical climate. Physical properties of leaves were measured, processed and analyzed for proximate principles as well as fiber fractions . Leaf length, width and petiole length was 6.60 ±0.17, 2.98± 0.18 and 1.86±0.19 cm for P. foetida and 5.49±0.17, 1.21±0.11 and 0.49±0.11 cm for H. auriculata. Dry matter of leaves was 23.04 and 16.98%, in the leaves of P. foetida and H. auriculata. On dry matter basis, nitrogen content found to be 2.12 and 4.11% with calculated crude protein 13.24 and 25.67%, in the leaves of P. foetida and H. auriculata. ADF , NDF , lignin, were 29.30, 39.23 and 9.81%, in P. foetida leaves and 16.89, 28.95 and 4.68%, in H.auriculata leaves. Ethar extracted oils was 5.95 and 12.28%, in the leaves of P. foetida and H. auriculata. Ash content which mainly constitute the minerals were 9.93 and 20.55% in P. foetida and H. auriculata., Nutritional values of H. auriculata leaves found better than P. foetida leaves with more protein, minerals, essential oils with less lignin for better digestability in the system. However, total dry matter and organic matter found more in P. foetida leaves. Thus, both P. foetida and H. auriculata , ethnomedicinal plant can be grown in small kitchen garden maintaining their well established nutritional quality and daily use in diet as medicinal plant.
Biography:
Will be uploaded soon.
Abstract:
Mung bean is rich source of protein, vitamins and certain minerals. Mung bean grains also exhibits a stronge antioxidant activity. Vitexin and isovitexin are the major compounds responsible for the antioxidant activity of mung bean. Due to their clinical importance, there is a need to develop a simple, sensitive and accurate methods for the determination of these compounds. Thus an attempt has been made to devolep a reversed-phase high-performance liquid chromatography method for the simultaneous determination of Vitexin and Isovitexin present in mung bean. The chromatographic system was optimized using an analytical Hypersil ODS column (150 x 4.6mm) with mobile phase 0.1% ortho-phosphoric acid (mobile phase A) and acetonitrile (mobile phase B) at flow rate of 1.0ml/min. Vitexin and Isovitexin were eluted at 9.52 and 10.25min and the chromatograms were extracted at 360.0nm. The method was validated for linearity ranges, precision, recovery, limit of detection (LOD) and limit of quantification (LOQ). The linearity for both compounds were observed from 5ppm to 100ppm with correlation coefficient coffeicient of 0.999, recovery for vitexin and isovitexin was 98.27 and 97.60%, respectively. The %RSD for interday precision (vitexin 0.98% and isovitexin 1.20%) and intraday precision (vitexin 1.42% and isovitexin 1.73%) was found to be satisfactory. The LOD values were 0.094 and 0.069mg/100g, while the LOQ were 0.312 and 0.230mg/100g, for vitexin and isovitexin, respectively. The developed and validated method was successfully applied for the detection and quantification of vitexin and isovitexin content present in two mung bean samples procured from different regions.
K Sai sruthi
Sri Padmavathi Mahila Visvavidyalayam, India
Title: Pharmacognostic and physicochemical studies of the roots of stereospermum suaveolens
Time : 18:40-18:55
Biography:
Sai sruthi completed her B.Pharmacy from C.L. Baid Metha College of Pharmacy, Chennai and M.Pharmacy from Vishwabharathi College of Pharmaceutical sciences, affiliated to JNTUK. I am pursuing my Ph.D from Sri Padmavati Mahila Visva Vidyalayam as a full time research scholar. I had attended one international and five national conferences.
Abstract:
The aim of the present study is to evaluate pharmacognostical parameters of roots of Stereospermum suaveolens which includes macromorphology, microscopical characters, physico-chemical constants and phytochemical screening. Roots of Stereospermum suaveolens were collected from Tirumala hills, Chittoor district, A.P and authenticated by the botanist Dr. Madhava chetty, Herbarium keeper, Department of Botany, Sri Venkateswara University, Tirupati, India and a specimen has been deposited in Department of Botany, Sri Venkateswara University, Tirupati, India. Roots were washed thoroughly, made into small pieces, shade dried and powdered in a wiley mill. The fresh root was used for the study of macromorphological and microscopical characters; whereas the dried root powder was used for determination of powder microscopy, physicochemical characterization and phytochemical screening. The dried plant material was subjected for determination of physicochemical parameters such as total ash value, acid insoluble ash, water soluble ash, alcohol soluble extractive, water soluble extractive value, pH, moisture content, foreign matter present, foaming index, etc. Fluorescent analysis and preliminary phytochemical studies had been carried out. Physicochemical parameters had shown characteristic values such as total ash value of 4.5%, acid insoluble ash of 1%, moisture content 16.5% and alcohol soluble extractive 8.8%. Preliminary phytochemical studies showed the presence of flavonoids and saponins. This information will assist in standardization for quality, purity and sample identification and further it will be used for pharmacological and therapeutical evaluation of the species.